e-tender for procurement of real time-pcr, automated tissue extraction machine and other accessories under animal resources development deptt, government of tripura , automated extraction system-- the instrument should have an open system having maximum of 16 magnetic rods that can purify up to 1-16 samples of dna, rna and protein from various samples such as bacteria, plant, plasmid, insect, food, saliva, water, blood, serum, plasma, stool, tissue, buccal swabs, buffy coat, cells, urine, formalin- fixed paraffin- embedded tissue (ffpe) and other body fluids and the purification should be cross­ contamination-free. - the system should have a cartridge holder adapter to perform a single sample without wastageof reagent & plastic ware. - the system should allow movement of super paramagnetic particles or magnetic beads instead of transferring liquids to enable efficient removal of contaminants. - it should allow purification and processing steps in 96-well deep well plates. - the system should accommodate processing volumes of 50 to 1000?l - the system should have heating for both lysis and elution. -the temperature range for both 96 well plates should be ambient to 100°c. - the system should be able to process up to 16 samples at a time. the processing time should be less than 45 min. - the system should have built-in uv disinfection along with a timer setting. - the system should eliminate hands-on processes such as ethanol precipitation, phenol/chloroform extraction, centrifugation or column purification, etc. - system should prevent exposure to hazardous chemicals. - the protocols should be selectable from a gui touch screen display. - the system should be portable and easy to move from one place to another place. system should havece-ivd approval , real time pcr system with pcr tubes-- system should have 96 well blocks with 0.2ml tubes, strips and 96 well pcr plate - the system should have led excitation with at least 5 led filters for excitation and 5 for detection filter, with 5 color multiplexing. the system should have - ramping rate of the machine should be 4°c/sec with temp. - accuracy: ± 0.1°c; temp. - uniformity: ±0.3°c (tested at 55°c); temperature range: 4-105°c with 0.1°c min increment - ramping rate : 4°c/sec (max) - gradient temp. range 1-36°c. - automatic 3d hot lid technology to reduce evaporation. hot lid temp. - range 30-110°c (adjustable default 105°c), automatic hot lid. - sample volume should be 5-100 ?l. - the detection system should be bottom-based photo multiplier tube technology (pmt). where individual target can be scanned. - scanning mode: designated line scan or entire plate. - not require any normalization method. it should be factory-calibrated. no passive reference should be needed. - customization of dye should be possible in the system. i.e., the addition of dye to the existing library should be possible - dyes used in system should be : f1: fam, sybr green i;f2: vic, hex, tet, joe,tamra;f3: rox, texas- red;f4: cy5, quasar-670; f5: cy5.5,quasar-705. - absolute quantification relative quantification, snp analysis, data automatic analysis, multiple file gene expression analysis, melting curve genotyping, hrm, customized parameters. - softwareto calculate ?ct, ?dct and rq fold values after completion of relative quantification assay for automatic post-data analysis and bar chart - compatible with reagents and plastic ware of any make/company. - plus/minus assay. snp (should be capable of automatic and manual call with simplified scatter plot). - capability to import standard curves in multiple experiments - capability to import and export experimental data in text or excel format for data analysis - provision with software with no download restriction. - software capable of being downloaded on multiple computers/desktops/laptops. - presence of local technical application support within 24 hours - the provision of providing the iqoqpq documents at the time of installation at free of cost along with instrument operation training. , single channel micropipette (0.1-2.5 ?l, 0.5-10 ?l, 10-100 ?l, 20 - 200 ?l 100-1000 ?l)-- variable volume microlitre pipettes wherein volumes can be continuously adjusted by rotating the plunger or thumb button. it has a click-stop digital system, reverse pipetting technique and separate, streamlined tip ejector. each pipette has to be tested & calibrated and should come with its own calibration certificate