bids are invited for rt pcr ( q3 ) • the system should be controlled by touch screen and external computer also. the software should come with a broad spectrum of optimized analysis algorithms including absolute and relative quantification, ducts method, pcr efficiency, allelic discrimination, endpoint detection as well as melt point and protein analysis. • system should have thermal silver block with gold plating 96 x 0.2 ml well format with gradient function, performs both real time and end point analysis with sample size volume capacity 5 –100 μl. • the system should have an array of 8 high performance optical fibres in a shuttle system to direct led light bundled by lenses to samples for lossfree excitation scanning for best uniformity and reproducibility. • depending on the application system filter module system should be freely configured and upgradable to both intercalating and proteinbinding dyes, with 12 different available colors as well as to hybridization ( fret ) probes. the system should be future-proof, being easily retrofitted on-site with additional color, fret or protein modules, and can thus be adapted to other fields of application at any time • the system should have an automatic software-controlled temperature ramp rate 8.0°c / second for heating and 6°c / second for cooling with a temperature range: 3-99°c & hot top heated lid. • the excitation source should be long live 4robust leds no preheating time with longer life& detection should be from high sensitive pmt ( photo multiplier tube ) • the temperature control accuracy should be ± 0.1 °c or better for best performance & temperature uniformity of ± 0.15 °c @ 55 °c after 15 sec • the system should be an open platform supporting all fluorescent chemistries and numerous applications & should be open for reagents of any make. • the system should come with at least 6 color-filters & must be flexible for multiplexing capability and support up to true 6 colors multiplexing without any rox dye needed. • to avoid condensation and the resulting loss of samples, the system should be equipped with a motorized heated lid with a temperature range up to 110 °c and guarantees, regardless of the consumables used an optimal contact pressure on the sample vessels during the entire realtime pcr run. • the system should have allelic discrimination by threshold cycle or by endpoint fluorescence values with auto-call feature. • the system should come with 2 warranty on device system&10 years long-term warranty of high-performance optics. • system should be supplied with computer system to analyze data by software. total quantity : 1