supply of one real time pcr, make thermofisher, biorad, abbott , real time pcr (make-thermo fisher, bio-rad, abbott): 1. thermal cycler with block of 96 well plate to run typical 0.2 ml reaction in flat, optical cap tubes, strips, and plates. 2. should be able to detect of all wells simultaneously through fixed device dedicated to each well by ccd/cmos. 3. should provide capability for multiplexing with 6 dyes: preferably syber green/ fam, vic/joe,tamra/cy3/ned, rox/jun/texared, cy5. 4.should provide quasar705 with option of passive reference dye as well suitability to new dye in market. 5. no internal reference dye should be required. true 5 colour multiplexing with use of 5 different flourophores without the need of addition of any internal reference dye 6. system should be open for chemicals and plastic wares from other manufacturers also so that different chemistries using taqman, molecular beacon, sybr green etc all can be performed. 7. should have multiple scan modes with a fast scan option for reading all wells in 3 seconds. 8. should have mode for melt curve analysis. 9. should have better sensitivity to detect less than 10 copy. 10. should have single white led as light source. 11. precision should be minimum 99.5% or better 12. advance user defined 6 zone temperatures for standardization of sample. 13. should provide temperature range 4- 99oc with accuracy ±0.2oc and uniformity of ±0.4oc or better within 10 sec of arrival at 90oc. 14. should have ramping rate 6oc/sec or better. 15. should offer working with reaction volume range of 10-50 ?l or better 16. system should have licensed software license. 17. should provide data application like gene expression analysis, snp genotyping, pcr quantification with standard curve, allelic discrimination etc. 18. should provide multiple experiment data analysis software, absolute and relative quantification 19. should be provided with external data access & analysis device : intel core i7 processor preferably latest version, latest operating system windows professional 64- bit, hdd1 20. should provide with reputed brand desktop dell/hp with 1tb memory, 8 gb ram and 2 kva online ups with 3 hrs back-up. 21. the system should be factory calibrated with no requirement of any further calibration system should iec & ce certified. 22. should provide sensitivity 1.5 fold & copy number 1 23. integrated touch screen panel on system along with usb support and with factory calibrated optics for minimal setup 24. should have a power supply of 100-240 v, 50-60 hz compatible with indian power supply 25. should have facility to connect through wi-fi and ethernet and backup on cloud. 26. system must have warranty of 5 years. 27. should provide service quote for annual/ comprehensive maintenance contracts for five years after completion of warranty period. 28. starter kit - which includes reagents like syber green dye and/ or taqman probes, plastic ware (tubes, tubes cap) and rt pcr kit, for atleast 2000 reactions. 29. accessories - latest laptop for offline data analysis and colour laser printer preferably hp.