thermal cycler (pcr machine) supply and installation of thermal cycler (pcr machine) , thermal cycler (pcr machine) , technical specifications • the thermal cycler can be 96 or 48 x 2 block or 32 x 3 block format with total 96 wells. • sample capacity: the thermal cycler should support use of pcr plates, pcr strip tubes as well as 0.2ml tubes. • the thermal cycler should support sample volumes ranging from 10?l to 100?l / tube. • the thermal cycler should have block ramp rate of 5 degree c/sec or better • dynamic ramping and peltier based heating and cooling • the thermal cycler should be able to set user defined annealing temperatures and it should have gradient feature to precisely set at least 6 different incubation target temperatures for pcr optimization. • the thermal cycler should have the auto restart facility for the events of power failure. • the thermal cycler should have block temperature range starting from 0°c – 100°c. • the thermal cycler should have programmable ramp rate with dynamic ramping so that all wells reach the designated temperature at the same time. • the thermal cycler to have a temperature accuracy ± 0.25 °c at 35°c – 99.9°c and temperature uniformity < 0.5°c. • the thermal cycler should have a programmable heat lid: heated lid should be programmable between 50 to 105°c. • memory: the thermal cycler to have provision to save up to 800-1000 protocols in the instrument. • the thermal cycler should have usb, ethernet interfaces and cloud network connectivity using wifi. • machine display: the thermal cycler should have a lcd display with touch screen • preferred optional technical specifications: ? the thermal cycler should have provision for generating automatic protocol based on user defined polymerase, primers and product length ? the instrument should have a security system to create username and password for each user as well as the password protection could be turned off under the administrative user validity and safety requirements • warranty: the thermal cycler and all its parts should have comprehensive warranty for one year. • the equipment should be ce or equivalent certified. • the equipment should be a well-tested and validated standard equipment and not custom made. • the equipment should come with a certificate of analysis and conformity. • documentation should include user/technical/maintenance manuals, list of important spares and accessories with their part number and price and list of recent end-users in centrally or state funded research/academic institutes in kerala or other parts of india. • readily available service support based in kerala from manufacturer (support document to be submitted from manufacturer side). • the vendor needs to provide the address of certified and authorized service center for the quoted equipment in/ nearest to thrissur, kerala. • the equipment should be installed, tested and demonstrated on-site at the requested location by authorized service agents of the manufacturer/ vendor. • the equipment should be delivered at kfri sub centre, kuzhur, kakkulissery, kuzhur p. o. thrissur, kerala – 680734.