rate contract of chemical reagents disposable and kits for hla lab-2 hla typing kits a, b.dr by ssp molecular techniques-demo required 3 positive controls for cross matching ( bag / inotrain ) 4 negative controls for cross matching ( bag / inotrain ) 5 celiac disease hla typing kits dq2& dq8 by ssp molecular techniques -demo required 6 hla b 27 pcr ssp technique-demo required 7 dna mini kit extraction from blood. 8 name of chemicals 9 proteinase k ( molecular grade ) 10 rabbit complement ( molecular grade ) 11 5% aquas eosin ( molecular grade ) 12 rpmi media -1640 with out l-glutamine ( molecular grade ) 13 propan -2-ol ( iso – propyl alcohol ) ( molecular grade ) 14 10 x tbe buffer ( molecular grade ) 15 taq polymerase ( molecular grade ) 16 ethidium bromide ( molecular grade ) 17 electrophoresis matrix low eeo agarose / powder ( molecular grade ) gelling temp. ( 1.5% ) 34.50c – 37.50c , moisture : ≤ 0.10%, sulfate : ≤ 0.15% eeo ( mr ) : 0.09-013, gel strength ( 1% ) :≥1, 200g / cm2, dnase / rnase: non detected , store dry ( 180c - 260c ) 18 lymphoprep density gradient separation media→ ready made , sterile & endotoxin tested solution. sod. diatrizoate – 9.1 % , polysaccharide – 5.7% density- 1.077±0.001g / ml, osmolality – 290 ±15m osm endotoxins- <1.0eu / ml 19 napathol as phosphate ( molecular grade ) 20 fast blue bb salt ( molecular grade ) 21 neutral red ar c15h17cln4 ( molecular grade ) 22 3, 3-diaminobenzidine ( dab powder ) c12h14n4 ( molecular grade ) 23 formalin ( ar ) 24 chloroform ( ar ) 25 distilled water 26 methanol-ar 27 lishmen stain power ( ar ) 28 nuclear free water ( molecular grade ) need smaller packing 29 immersion oil ( microscopy grade ) 150 ml 30 savlon liquid soap 31 hand sanitizer antiseptic solution. 32 metabisulphate ( molecular grade ) 33 phenol red ( molecular grade ) 34 barium sulphate 35 urea 36 dna ladder 100bps ( molecular grade ) 37 ammonium choloride ( ar ) 38 pot. hydrogen carbonate ( ar ) 39 edta powder ( ar ) 40 sodium hydroxide pallets ( ar ) 41 sodium chloride nacl ( ar ) 42 sodium dodeyle sulfate ( ar ) 43 hydrogen peroixde ( ar ) 44 potasium dihydro phosphate ( ar ) 45 disodium hydrogen phosphate ( ar ) 46 disodium hydrogen phosphate dihydrous ( ar ) 47 ethanol molecular grade ( ar ) 48 isoamyl alcohol ( molecular grade ) 49 sodium hydroxide pellets 50 surface cleaning solution 51 name of disposable items 52 disposable dropper up to 2 ml mark 53 15 ml conical graduated tube with screw cap & label -sample required 54 rabbit complement cups 55 disposable tips yellow upto 1-10 ul ( setrile tips in boxes dnas, rnas free ) 96x10 -sample required 56 disposable tips white upto 10-100 ul ( setrile tips in boxes dnas, rnas free ) 96x10-sample required 57 disposable tips blue upto 100-1000 ul ( setrile tips in boxes dnas, rnas free ) 96x10-sample required 58 terasaki trays ( 60 wells ) 59 ependrof tube autoclavable with conical bottom 1.5 ml with snap lock frosted cap-sample required 60 cryolabels for ependrof tubes 61 filter paper watman no. 0 size 12.5 cm 62 nitrile powder free gloves 6.5 no ( sterilized ) 63 nitrile powder free gloves 7 no ( sterilized ) 64 shoe cover ( plastic disposable ) 65 pcr tubes. 0.2-0.5 ml with lid. -sample required 66 tissue paper roll 67 micro pore roll. 68 kim wipe papper 69 parafilm ( 41n x 125 ft ) roll 70 temperature recording paper of -80 degree deep freezer 71 almunium foil 72 50 ml conical graduated tube with screw cap & label