rate contract of supply of kits , chemical and reagent and disposable for hla labn-2 hla typing kits a, b.dr by ssp molecular techniques-demo required 3 positive controls for cross matching ( bag / inotrain ) 4 negative controls for cross matching ( bag / inotrain ) 5 celiac disease hla typing kits dq2& dq8 by ssp molecular techniques 6 hla b 27 pcr ssp technique 7 name of chemicals 8 proteinase k ( molecular grade ) 9 lymphoprep density gradient separation media? ready made , sterile & endotoxin tested solution. nsod. diatrizoate – 9.1 % , polysaccharide – 5.7% ndensity- 1.077±0.001g / ml, osmolality – 290 ±15m osm nendotoxins- <1.0eu / ml 10 nuclear free water ( molecular grade ) need smaller packing 11 dna ladder 100bps ( molecular grade ) 12 napathol as -b1 phosphate -c18h15brno6p ( molecular grade ) 13 fast blue bb salt -c17h18n3o3cl ( molecular grade ) 14 formalin ar 15 10x tbe buffer ( molecular grade ) 16 3, 3-diaminobenzidine ( dab powder ) c12h14n4 ( molecular grade ) 17 chloroform ar 18 distilled water 19 methanol ( molecular grade ) 20 lishmen stain power ( ar ) 21 immersion oil ( microscopy grade ) 150 ml 22 savlon liquid soap with dispenser 23 hand sanitizer antiseptic solution with dispenser 24 sodium meta bisulphate ( molecular grade ) 25 surface disinfectant liquid aldehyde free alkaline disinfectant for cleaning surfaces. it contains benzalkonium chloride ip, octyldecyldimethyl ammonium chloride, dioctyl dimethyl ammonium chloride with other ingredients with spray dispenser 26 disinfectant for instrument with spray dispenser 27 taq polymeraser 28 diluent for dna extraction ( molecular grade ) 29 rpmi media -1640 without l-glutamine and without mgcl2 ( molecular grade ) 30 rabbit complement ( molecular grade ) 31 electrophoresis matrix low eeo agarose / powder n ( molecular grade ) gelling temp. ( 1.5% ) 34.50c – 37.50c , moisture : = 0.10%, sulfate : = 0.15% neeo ( mr ) : 0.09-013, gel strength ( 1% ) :=1, 200g / cm2, dnase / rnase: non detected , store dry ( 180c - 260c ) 32 isoamyl alcohol ( molecular grade ) 33 name of disposable items 34 disposable dropper up to 3 ml mark 35 15 ml conical graduated tube with screw cap & label certified with dnase, rnase, pyrogen free ( sterilized ) 36 disposable tips without filter 1-10 ul ( setrile tips in boxes dnas, rnas free ) 96x10 37 disposable tips without filter 10-100 ul ( setrile tips in boxes dnas, rnas free ) 96x10 38 disposable tips without filter 100-1000 ul ( setrile tips in boxes dnas, rnas free ) 96x10 39 disposable filter tips of 20-200 ul sterile dnas, rnas free in boxes 40 terasaki trays ( 60 wells ) 41 ependrof tube autoclavable with conical bottom 1.5 ml with snap lock frosted cap-dnas, rnas & pyrogen free sterile 42 ependrof tube autoclavable with conical bottom 0.5 ml with snap lock frosted cap-dnas, rnas & pyrogen free sterile 43 almunium foil 72 meter 44 temperature recording paper of -80 degree deep freezer