procurement/execution of sequencing and analysis service ( genotyping by sequencing ) at icar-igfri , jhansi , sequencing and analysis services ( genotyping by sequencing ) service includes : 1 . collection of leaf samples- total number of 150 samples 2 . dna isolation , quality check . 3 . preparation of bar coded libraries using standard protocols ( double digestion/rad ) 3 . sequencing to be carried out on illumina hiseq or other platforms generating a minimum of 100 bp-150bp long reads for each sample . 4 . bioinformatic analysis ( statdard quality check , trimming of sequences , read alignment and snp calling and other relevant bioinformatics analysis as per requirement ) . 5 . all the generated data should be provided ( raw data/reads , assembled reads , data of snp analysis/snp calling , variant comparison ) . note-the quantity of sample may increase or decrease .