tender for supply of specialized laboratory chemicals, reagents, culture media and allied ancillary items for ivf & embryo transfer laboratory under paschim banga go-s ad bikash sanstha, lb-2, sector-iii, salt lake city, kolkata-700106 , oocyte and embryo wash media 25 ml, hepes or mops dual-buffering systems to maintain a stable external ph without needing a co₂ incubator during ovum pick-up (opu) or washing, each manufacturing lot must support a baseline cleavage rate of ≥75% and transition to a blastocyst rate exceeding 20% to 50% , buffered oocyte maturation medium 25 ml, hepes combined with sodium bicarbonate (nahco₃) ph 7.3-7.4, fortified with precise, low doses of follicle-stimulating hormone (fsh) and luteinizing hormone (lh) to prompt meiotic maturation , ivf fertilization medium 25 ml vial, sodium bicarbonate-buffered, strictly regulated between 7.20 and 7.40 at the standard animal core temperature , embryo culture medium 25 ml vial, sodium bicarbonate-buffered, ph range: regulated within an optimal range of 7.20 to 7.35 , embryo transfer and transportation medium 25 ml vial, heavy hepes or mops concentration combined with a baseline of sodium bicarbonate, strictly regulated between 7.30 and 7.45 , mineral oil for medium overlay 100ml bottle, purified, sterile, and non-toxic oil to cover microdrops of culture media during in vitro embryo production (ivp) , sperm separation medium (upper layer) 6 ml vial, ormulated with 40% to 45% v/v silane-coated, colloidal silica particles suspended in a balanced salt solution, heavy hepes or mops buffer. , sperm separation medium (lower layer) 6 ml vial, ormulated with 80% to 90% v/v silane-coated, colloidal silica particles suspended in a physiological salt solution, heavy hepes or mops buffer. , opu media (ovum pick-up medium) 500ml, heavily hepes-buffered, strictly regulated between 7.30 and 7.45 at ambient and handling temperatures. , semen preparation media 20 ml, heavily hepes- or mops-buffered, rictly regulated between 7.20 and 7.50 at ambient and processing temperatures , nunc ivf multidish non-treated, 4 well dish, 1.9 cm2/well, 120/cs, exactly 1.9 cm² per well, approximately 1.0 ml to 1.9 ml maximum volume per well, fabricated entirely from medical-grade, high-clarity usp class vi polystyrene. , add in ivf medium 110 ul for 10 ml ivf, 2 ml, strictly sodium bicarbonate-buffered (nahco₃), ightly regulated between 7.25 and 7.40, fortified with bovine serum albumin (bsa) , add in ivf medium 440 ul for 10 ml ivf, 2 ml, strictly sodium bicarbonate-buffered (nahco₃), ightly regulated between 7.25 and 7.40, fortified with bovine serum albumin (bsa) , dilution percoll media, 10 ml exactly 9 parts raw percoll (100%) blended with 1 part 10× concentrated salt solution (e.g., 1.5 m nacl or 10× concentrated sperm-talp / tyrodes medium). , gradient for conventional semen, 10 ml, heavily buffered with hepes or mops. tightly controlled between 7.20 and 7.60 at room/handling temperature. , gradient for sexed semen, 10 ml, , heavily buffered with hepes or mops. tightly controlled between 7.20 and 7.60 at room/handling temperature. , cup filter for et catheter pack of 12 can be set on a y-tube system with clamp flow control. mesh size: 75 μm , 2 waywoerlein catheter l 75 cm ch18 non sterile , tubing without filter for foley catheter ch18 or ch 20 , embryo flushing media, 1 ltr , embryo holding media, 20 ml , aliquot- for ivf medium 2 ml , petri radiated plates 100 mm x 100 mm x 15 mm for finding and evaluating embryos. pack of 10, molded from premium, heavy-walled virgin crystal polystyrene (ps). , petri radiated plates 35 mm x h 10 mm for finding an embryo after flushing.pack of 10 molded from premium, heavy-walled virgin crystal polystyrene (ps). , sterile et plugging rodshort yellow plugging rod (13 mm), fits 133 mm straws pack of 5 , non-sterile flexible mini et straw 133 mm non-sterile pack of 5 , roll of sanitary sheaths to protect the sheath during insemination and prevent contact between the ai sheath and the animal (bovine). , ted - opu line tube 2 mtr. (ptfe) 20g , wta follicular aspiration needle 18g , ted - follicular aspiration needle 18g (for opu) , wta - follicular aspiration needle 20g (for opu) , follicular aspiration needle 20g (for opu) , cervix dilator , embryo freezing media ethylene glycol and sucrose , direct et mini pastel yellow straws printed dt pack of 40 , micro aspiration device , et syringe for ø 3 mm sheath for cut straws (s) , et sheath ø 3 mm. pack of 40