nit for annual rate contract for supply of media for ivf required for art centre for department of obstetrics and gynaecology at aiims nagpur , item name with specification , 80% gradient ? density gradient media used to separate cells, organelles, membrane vesicles, viruses, proteins, nucleic acids, and lipoproteins. ? density gradient centrifugation (dgc) is a popular procedure used in assisted reproductive technology (art) to select sperm based on their density. capacity 12 ml , 40% gradient ? used to separate cells, organelles, membrane vesicles, viruses, proteins, nucleic acids, and lipoproteins. ? density gradient centrifugation (dgc) is a popular procedure used in assisted reproductive technology (art) to select sperm based on their density. capacity 12 ml , sperm wash ? used to wash sperm and isolate motile sperm using the swim-up method. ? the ideal sperm preparation medium helps maintain a stable ph and osmolality while providing the proper physiological environment for sperm survival. 50 ml capacity , sperm freezing media ? hepes-buffered media which also contains physiologic salts, glycine, glucose, lactate, and the cryoprotectants glycerol (15% and 27%). ? sucrose and human serum albumin (4.0g/l, medicinal substance derived from human blood plasma) to protect the sperm from damage during the freezing procedure. 10 ml , sperm mobil ? spermmobil is a hepes-buffered reagent containing low bicarbonate. ? it is used for in-vitro examination of sperms of necrozoospermic ejaculates as well as of immotile sperms isolated from testicular tissue 0.05 ml , sperm morphology kit ? pre stained slides for sperm morphology assessment ? spermatozoon is an intricate motile cell with a well-defined head, neck and tail. ? the shape of the normal sperm is a reflection of proper spermatogenesis in the testicles , dna fragmentation kit (sperm chroma kit) ? halosperm: an in vitro diagnostic kit that can measure dna fragmentation without complex laboratory equipment , sperm vitality kit ? the fluovit kit enables the evaluation of sperm vitality in fresh or thawed semen samples. , semen fructose kit ? a sperm fructose kit, also known as a semen fructose test, measures the amount of fructose in human semen or seminal plasma. ? fructose is an energy source for sperm motility and metabolism, and its absence can indicate issues with the seminal vesicles or ejaculatory duct. , sageone step ? one-step media is a type of culture media used for human embryo culture. ? the composition of one-step media includes:magnesium sulfate, potassium chloride, potassium phosphate, sodium chloride, amino acids, l-alanyl-l-glutamine, calcium-l-lactate, glucose, sodium pyruvate, and sodium bicarbonate 20 ml , embryo thawing kit ? for thawing of human zygotes and cleavage stage embryos ? 1 m sucrose thawing medium with 12 mg/ml human serum albumin ? 0.5 m sucrose thawing medium with 12 mg/ml human serum albumin ? mops , embryo freezing kit ? vitrification media kit includes:equilibration solution vitrification solution , hylase1ml ? hyalse contains 80 iu/ml of hyaluronidase in hepes buffered htf with hsa.for the removal of cumulus and corona cells fromm the oocyte prior to icsi procedure. , pvp 7% 0.5ml ? -pvp reduces the sperm motility simplifying the immobilization and easing the handling during intracytoplasmic sperm injection (icsi). ? it also prevents sperm from sticking to the injection pipette (v-icsipet) and gives better control of media and sperms. , oil100ml pet ? oil is a ready to use light paraffin oil for art-procedures and applicable for short and long term cultures up to 6 days. , oocyte freezingkit ?x 1.5 ml basic solution vial (bs) ? 1 x 1.5 ml equilibration solution vial (es) 2 x 1.5 ml vitrification solution vial (vs) 10 ml , oocyte thawing kit ? media for oocytes, free from protein, compose of synthetic and plant derivatives, hydroxy propyl cellulose and to be used as a non-protein supplement. sterility tested. ? mouse embryo assay tested. contains sucrose, sodium lactate, sodium bicarbonate. antibiotic. medium is ready to use. storage at 2-8°c. 10 ml , oocytes activation media. ? media use for activation of oocytes. maintain a stable environment during. washing oocytes. antibiotics. medium is ready to use. store at 2-8 ?c. protect from light , in vitro maturation media. ? in vitro maturation media is for the in-vitro maturation of immature oocytes, subsequent fertilization, and embryo development , laboratory equipment disinfectant ? disinfectant for medical equipment in in-vitro fertilization (ivf) laboratories, including co2 incubators and laminar flow hoods. ? its a transparent, odorless, ready-to-use, alcohol-free, non-voc releasing, and non-fragranced mixture. ?its effective against bacteria, yeasts, viruses, and mycobactericidal. ? it doesnt corrode metals or affect organic soil. , laboratory surface disinfectant ? non toxic, non odour, non alcohol, no acetone, no aldehyde. compatible with metal rubber and plastic. ? effective against hepatitis b, hiv, rotavirus with in 1-2 minutes, microbectrium within 4-6 minutes bacteria fungi between 12-17 minutes, ready to use or dilution. : , laboratory hand disinfectant. ? non toxic,safe disinfection for hands. non alcohol , no odour. effective against hepatitis b, hiv, rotavirus with in 1-2 minutes, microbectrium within 4-6 minutes bacteria, fungi. ? neutralizes bacteria which causes bad smell does not dry the skin. ready to use. storage : room temperature. , embryo glue ? embryoglue has the basc composition of a rich blastocyst culture medium and contains recombinant albumin and a high concentration of hyaluronan ? it can be used for transfer of all embryo developmental stages, including cleavage embryos, blastocysts after assisted hatching, biopsied embryos and cryopreservation. , double distilled sterile water ? purified by steam distillation for use as a research laboratory reagent. , gamete buffer ? retrieval, holding and washing of oocytes is ready-to-use and especially designed for in vivo use during oocyte retrieval. ? flush is fully synthetic and does not contain human serum albumin (hsa) or antibiotics. ? when supplemented with heparin, the medium is used to prevent clotting if blood is present after flushing the follicles. , fertilization ? supports both gametes during fertilization and promotes sperm function , cleavage ? formulated to support early embryo development , blastocyst ? designed to meet metabolic needs after embryo genome activation , deionised water ? water is passed through a reverse osmosis membrane and a deionizing membrane. ? does not conduct electricity. ? ultrapurified. , 70%iso propyl alcohol ? isopropyl alcohol (ipa) is effective as a disinfectant ? issolve more slowly, penetrate cells, and kill bacteria