Laboratory Equipment and Supplies, Laboratory Equipments, PCR and RT PCR Products, Scientific Instrument, Universal Testing Machines, Scientific Equipment, Chennai-Tamil Nadu

Tender For 1. An Integrated System For Both Real-Time Pcr & Post-Pcr (End-Point) Analysis UsingIn-Built Peltier Based Pcr Machine.2. It Should Be Possible To Use Computer For System Control, Operation, Analysis, Net-WorkingOf Multiple System.3. Thermal Cycling System :§ Peltier-Based§ Instrument Should Be Quoted With 96 Well Block.§ Instrument Must Also Have Option To Upgrade To 384-Well And No Service Visits OrRe-Calibration Are Necessary After A Block Change.4. Optical System :§ Detection By Ccd/Pi Camera And Excitation By Halogen/Led Lamp.§ The Light Source Should Be User-Interchangeable That Is Readily Accessible And CanBe Replaced From The Front Of The Instrument.§ Should Have More Than 5 Excitation (450–670 Nm) And 5 Emission (500–720 Nm)Filter Sets To Enable Collection Of Up To 10 Unique Combinations Of WavelengthsDuring A Single Run For Multiplexing.§ System Should Able To Collect Data For All Filters For All Wells Regardless Of PlateSetup.5. Sensitivity : The System Should Be Able To Detect 1 Copy Of Template For A Single Reaction6. Chemistry Support: The Instrument Should Be Open System Capable Of Running VariousChemistries So That Different Chemistries Using Taqman, Sybr Green Etc. The InstrumentSoftware Will Allow Both Taqman® And Sybr® Chemistries In The Same Run By CollectingDifferent Filter Colors For Pcr Versus Melt Curve Stages.7. Data: The Instrument Software Provides Raw Fluorescent Data And Multicomponent(Segregated By Dye Type) Data For Troubleshooting Of Experiment.8. Dyes Calibrated: System Should Provide An Option To Use More Than 10 Different DyesCombinations. The System Should Provide Addition Of New Dyes Without Any HardwareChange Within The Wavelength Range.Passive Reference Dyes: Any Calibrated Dye Preferably Rox Should Be Used As OptionalUse Of Passive Reference Dye For Normalization.10. Run Time: The Instrument Should Be Designed To Complete A 40 Cycle Real-Time PcrReaction And Fast Chemistries In A 384-Well Plate On Fast Block In Under 35 Minutes.Instrument Can Also Run In Standard Ramping Mode With Standard Chemistry.11. Reaction Volumes : 5-20 Μl For 96 Well Μl Reactions)12. Ramp Rate Should Be Adjustable13. Temperature Range : 4°C - 100°C14. Uniformity : +/- 0.50°C15. Supported Plastic Ware :0.1Ml 96 Well Plate/ Single Tubes With Caps/8Tubs Strips16. Consumable Support: Vendor Is Able To Supply All The Necessary Consumables ToPerform Real-Time Quantitative Pcr And Snp Genotyping, Including Pcr ReagentsDesigned For Use With The Fluorogenic 5’ Nuclease Assay, Pcr Reagents Designed For UseWith Sybr® Green I Dye Assay Chemistry, Fluorogenic Probes, Reaction Plates AndAdhesive Plate Sealing Covers. Pcr Reagents Are Also Available With An Optional PassiveInternal Reference Rox Dye To Minimize Well-To-Well Variability. The Supplier Should Also BeAble To Design And Provide The Taqman Assays For The Dna/Small Rna (Custom Assays)Templates Of Our Interest17. Installation Specification: The Instrument Has Real-Time Quantitative Pcr InstallationSpecifications Which Demonstrate The Ability To Distinguish Between 6,667 And 10,000Template Copies 1.5 Fold Sensitivity) With A 99.7% Confidence Level.18. Softwares Application :Absolute QuantitationRelative QuantitationHigh Resolution Melt Curve.Allelic Discrimination/Snp (Single Nucleotide Polymorphism) DetectionPlus/Minus Assays That Utilize Internal Positive Controls19. Primer & Probe Designing Software That Designs Probes And Primers In A Manner Such ThatPcr Is Carried Out Under Universal Thermal Cycling Parameters Irrespective Of TemplateDna To Eliminate The Optimization Of Pcr Condition For Running 96 Different Samples On ASingle 96 Well Plate In A Single Run Should Be Provided.20. Training, Service And Application Support: The Vendor Should Have A Good Service AndApplication Support Back Up Along With Instruments To Provide An Effective ApplicationRelated Troubleshooting And Support. The Vendor Should Provide Comprehensive TrainingAt Training Centre On The Operation Of The Instrument, Chemistry Options And Software

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