Tender For Supply Of Nanopore., Bhopal-Madhya Pradesh

Tender For Supply Of Nanopore.. => Limited; 1 Specification for Nanopore 1. The provided NGS sequencer should be an electronic analysis system for use in scientific research. 2. Sequencing chemistry should be based on the advanced sensing technology and involves direct molecular analysis of native DNA/ RNA or amplified DNA/cDNA products. 3. The sequencer should be capable of delivering flexible, on demand, portable or in lab sequencing when plugged to a compatible laptop or computing system. 4. Setting up the sequencer should require minimal to no facility upgrades. 5. Simple plug and play device and needs a minimal IT infrastructure, small footprint, suitable for any lab. 6. It should allow users to experience Simple library preparation, Real-time analysis and Biological insights from long reads. 7. The sequencer should allow to read the length of nucleic acid presented to them from short to ultra-long (longest >4 Mb). 8. Sequencer should allow a flexible run-time of 1-72 hour or better. 9. It should be capable of delivering total data output of up to 20- 25GB or better. 10. Flow cell should be capable of providing on-demand, long-read sequencing to suit all experimental requirements and should have the flexibility of reusing. 11. The sequencer should be compatible with latest sequencing chemistry which provides accuracy of 99.5%. 12. Simple and Rapid workflows, PCR-free library preparation protocols and multiplexing of up to 96 samples in a single flow cell using barcodes. 13. Should be compatible for both DNA/ RNA based sequencing applications such as Whole Genome sequencing, Targeted sequencing, Metagenomics (16S and Whole genome metagenome), cDNA/RNA sequencing. 14. The sequencer should allow real-time sequencing and base calling for rapid access to results. 15. The sequencer system should generate standard data output formats which is compatible for all downstream analysis software. 16. The sequencer should come with all the required softwares preloaded for sequencing and basecalling. 17. The package should come with onsite installation and training support. 18. The sequencer should come with 5 Flow cells (atleast 800 pores per flow cell should come under warranty), library preparation kits for DNA which also allows for processing ds cDNA, Wash kit and control expansion kits and other reagents. 19. The kits should also come with accessory NEB reagents for making amplicon, WGS and ds cDNA library prep. NEB Blunt/TA Ligase Master Mix - 50 RXN, NEBNext FFPE DNA Repair Mix - 24 RXN, NEBNext Ultra™ II End Repair/dA-Tailing Module - 24 RXN, NEBNext Quick Ligation Module - 20 RXN 20. The channel partner should be able to provide the required technical support and should have the certificate from ONT. 21. The instrument should be supplied with PC of following configuration or better- I7 14700F, ASUS B760M AYW WIFI D5 , DDR5 32GB RAM XPG 7, 1TB SSD Gen4, ASUS Graphic Card RTX 4070 12GB, SMPS 750W Ant esports, , Cabinet Gaming, Azebronics Liquid Cooler 360, Viewsonic 27 VX2779, KEYBORD+MOUSE .

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