Procurement Of Digital Pcr (Droplet) Under Hadp Project- 21, At Division Of Fruit Science, Main Campus Shalimar, Skuast-K. , Procurement Of Digital Pcr (Droplet) Under Hadp Project- 21, At Division Offruit Science, Main Campus Shalimar, Skuast-K. , Technical Specifications , The Digital Pcr System Is An All-In-One Fully Integrated System Equipped With A Partitioning Module, Thermal Cycler, And Images In A Single System , Digital Pcr System Should Be Equipped With A Control Software Using A Lcd Touch Monitor , System Should Able To Use One Plate With A Multiplexing Ability Up To Five Channels, Multiplexing Up To 5 Different Dyes For Single Well Without The Need Of Customization Of Dyes Or Wavelength , System Should Offer Below Excitation And Emission Wavelength For Light Source And Detection System , Digital Pcr Should Perform Excitation And Detection Of Up To 2 Fluorescent Dyes Per Partition (Green, Yellow). , System Should Be Equipped With An Independent Reference Channel For Counting The Number Of Filled Partitions And Determining The Analyzable Volume. , System Should Use High Power White Led For Illumination And Image Acquisition Is Performed Using Cmos Camera With 6.3 Mp. , Digital Pcr System Shouldanalyze A Full Plate Under 2 – 3 Hours , System Should Be Compatible With Probe-Based Detection Chemistry And Eva Green Chemistry, Vendor Should Provide Validated Master Mixes To Be Used On Digital Pcr System , Digital Pcr System Should Work With Nano Plate Technology, Should Be Compatible With 8.5K And 26K Nanoplates, Which Has A Total Of 8,500 And/Or 26,000 Partitions. , Nanoplate Should Be In Standard Sbs Format. The 8.5K Nanoplate Holds A Total Of 96 Reactions (96-Well) And The 26K Nanoplate Up To 24 Reactions (24-Well Format) With Minimum 8 Wells. , Pcr Well Plate Should Have An Input Reaction Volume: - 12 Microliter And 40 Microliter. , Precision Of ±10% Should Be Achieved On The System , Software Should Be Accessed Through A Server Network And Also Installed In Individual Computers For Analysis Purposes , System Software Should Allow Users To Access All Images Acquired By The Digital Pcr System For Each Channel , Software Of The System Should Offer Data Visualization As A Table, Histogram, Scatterplot, Heatmap And Bar Chart, Software Should Display Fluorescence Amplitude Per Partition In A 1D Or 2D Scatterplots (For Multiplex Data). , System Software Should Computes Concentration In Copies/Ul For Each Sample. , Software Shouldautomatically Set Thresholds Across The Entire Plate Or For Individual Wells. Alternatively, The User Can Manually Set The Thresholds. , Software Should Offer Data Analysis For The Calculation Of Mutation Frequency, Gene Expression Fold Change, Copy Number Variation As Well As Genome Editing , All Analysis Data And Images In The Software Should Exported As A .Csv File (For Use In Microsoft Excel Or Similar Program) And .Png Or .Svg (For Images) And Pdf Format , Software Of The System Should Be With Guidelines Of 21 Cfr, Vendor Should Provide Document For The Same As And When Required , Number Of Installations:- Vendor Should Have More Than 20 Installations In India. , Warranty:- One Year From Date Of The Installation Of The System , Application Training Should Be Provided To Two To Three Staff Members By Trained Application Team Of The Vendor / Manufacturer , Note:-The Rates Quoted Are Inclusive Of All Carriages Of Materials (Mechanical/Manual) From Source To Site Work And All Extra Chargescomplete In All Respects.
