Tender For Media And Consumables Of Embryology Lab And Andrology Lab For Department Of Reproductive Medicine - Follicle Flush Buffer Media: Washing media having both optimal and a stable pH environment for flushing ovarian follicles during oocytes collection, suitable for flushing needles. Specially having HEPES-buffer. Medium is ready to use and must be supplemented with protein. Store at 2-8 ֯ C. Protect from light Embryo Vitrification Pack: Sterility tested & DMSO based/DMSO Free media. Mouse Embryo Assay tested. Medium is ready to use.Store at 2-8 °C. Protect from light. Embryo Vitrification Thaw Pack: Sterility tested. Mouse Embryo Assay tested. Contains Sucrose, Sodium lactate, Sodium Bicarbonate. Antibiotics. Medium is ready to use. Store at 2-8 ֯ C. Protect from light. Sperm Preparation Media: Supplemented with: Human Serum Albumin. Sodium pyruvate. A Bicarbonate based buffer for sperm preparation and storage. HEPSES Buffer. Contain Antibiotics. Medium is ready to use. Store at 2-8 ֯ C. Protect from light. Media should be in alkaline Ph(8-8.5) for better results Density Gradient Media: Designed specifically for sperm preparation and isolation of viable spermatozoa using density gradient separation. Each packaged in a kit with one vial of each 40% and 80% densities. Antibiotics. Medium is ready to use. Store at 2-8°C. Protect from light. Media should be in alkaline Ph(8-8.5) for better results. Surin up media Sperm Freezing Medium Sterility tested: Mouse Embryo Assay tested. Sperm survival tested. pH 7.3-7.5. A HEPES buffered cryopreservation medium with glycerol as the cryoprotectant used for freezing washed sperm, MESA and TESA samples. Antibiotics. Medium is ready to use. Store at 2-8°C. Protect from light. Culture Oil: Ensures a high-quality culture system with extensive washing and testing during manufacture. Reduces osmotic stress caused by evaporation. Helps maintain pH stability and used as an oil overlay for culture media during IVF and ICSI procedures. Sterility tested. Medium is ready to use. Store at 2-8 °C. Protect from light. PVP Medium: This solution used to reduce the motility of sperm making it easier to catch them with an ICSI pipette with bicarbonate buffered medium containing 7% polyvinyl pyrrolidine. Sperm survival tested. Sperm immobilization tested. Antibiotics. Medium is ready to use. Store at 2-8°C. Protect from light. Natural Alternate to PVP Media: (Sperm Slow Media) Hyaluronidase: (O ocyte Denudation) Single Step Media: A single step media for uninterrupted embryo culture up-to blastocyst stage. Sterility Tested, Mouse Embryo Assay Tested, Contain Antibiotics, Medium Ready to use, Store at 2-8°C. Transfer Media: Based on buffered with HEPES, Transfer media having hyaluronate Endotoxin level <0.1 EU/ML, Antibiotics, Storage : 2-8°C DNA Fragmentation kit: Ready to use. Storage: Room temperature. Sterile water: Suited in IVF lab for the cell and tissue culture, co2 incubator , humidity pan, cleaning the surface of the laminar hood. Endotoxin tested limit less than 0.005 EU/ml. MEA up to 96 hours ≥80%. Storage : Room temperature Laboratory disinfectant: for co2 incubator and laminar hood. Non toxic, safe cleaning for co2 incubator and laminar hood. No alcohol, no odor. Effective against hepatitis B, HIV, rotavirus with in 1-2 minutes, microbectrium within 4-6 minutes bacteria fungi between 12-17 minutes. Laboratory Hand disinfectant: Non toxic, safe disinfection for hands. Non alcohol, no odor. Effective against hepatitis B, HIV, rotavirus within 1-2 minutes, microbectrium within 4-6 minutes bacteria, fungi. Neutralizes bacteria which causes bad smell does not dry the skin. Ready to use. Storage: Room temperature Lab disinfectant: for surface cleaning Non toxic, non alcohol, no acetone, no aldehyde. Compatible with metal rubber and plastic. Effective against hepatitis B, HIV, microbectrium bacteria fungi, Ready to use or dilution. : Room temperature DISPOSABLES REQUIRED FOR ART FACILITY IVF Test Tubes: Polystyrene Round bottom 14ml. Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non- pyrogenic at less than 0.25 end toxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Test Tubes: Polystyrene Round Bottom 6ml. Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non- pyrogenic at less than 0.25 endtoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Conical Test Tubes 5ml: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 end toxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Transfer Pipette 3ml: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 end toxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Semen Container: USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Petri dish 35mm: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Petri dish 60mm: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Petridish 60mm: Centre Well Dish. Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Petri dish 90 mm: Sterility assurance level to be 10⁶.USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique IVF Grade Four Well Dish: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique Echo tip Ovum Pick up Needle: Single/Double lumen & Catheter Ovum Picks up Set (with valve) Tube 60-100 cms Needle 30-35 cms. Gauge 17. B. Thin tip Embryo Transfer Catheter 4.5 cms: (a)- Soft (visible on USG). (b)- Rigid (visible on USG). (c) - Trial embryo catheters. Storage : Room temperature ICSI Dish: Sterility assurance level to be 10⁶. USP class VI tested polystyrene. Non-pyrogenic at less than 0.25 endotoxin units/ml. Qualified plastic ware for in vitro fertilization (IVF) and assisted reproductive technique. MEA tested Cryo Vials 1-2 ml: (semen freezing). Storage: Room temperature IVF Grade Syringes 1 ml. Storage: Room temperature. IVF Grade Syringes 5 ml. Storage: Room temperature ICSI DISPOSABLES: Holding Pipettes (Same make as injecting pipette) Inner diameter 30 μm and outer diameter 120 μm with distal tip angle 35º. Better support for the oocyte or embryo and reduces distortion during micromanipulation Injecting Pipettes: (Same make as holding pipette). To reduce sperm adhering to glass. Inner diameter 5.4 μm and outer diameter 7 μm with distal tip angle 35º. Precision injection pipette offer reduced tip flexibility and parallel walls for precise sperm control. Storage: Room temperature DENUDING PIPETTES: Denuding cumulus mass from the oocytes prior to ICSI to check fertilization or for micromanipulation of oocytes embryos, blastocyst and blastomeric. Flexible polycarbonate pipette are designed for use with reusable pipetting handles. Supplied sterile FLEXIPET/DENUDING PIPETTES Inner Diameter - 130/135 μm, Inner Diameter - 140/150 μm Inner Diameter - 170/175 μm, Inner Diameter - 225/250 μm, Inner Diameter - 275/300 μm Sterile Tips with white filter &: RNase Dnase pyrogen free & DNA free should be fit out micropipette. (1000μl). Storage: Room temperature (single pack). Sterile Tips with white filter &: Rnase Dnase pyrogen free & DNA free should be fit out micropipette ( 0.5-10μl) Storage : Room temperature (single pack). Sterile Tips with white filter &: Rnase Dnase pyrogen free & DNA free should be fit out micropipette ( 20-100μl). Storage: Room temperature (single pack). Lint Free Tissue Paper: Storage : Room temperature. Open Device for Vitrification: Storage of vitrified human oocytes and embryos. Mouse embryo assay tested. Storage: Room temperature Cryo Labels: Pre cut labels (white colour) Size: 1.28×0.50 inches. Precut, Peel off labels sized to fit micro centrifuge tubes and other container, Ideals for 1.5-2.0ml or lager tubes. Freezable in liquid nitrogen, Chemically resistant. Storage: Room temperature. Cryo Marker: For marking cryo vials and straws. Alcohols free, Storage: Room temperature Goblet: Goblet for frozen straws (oocytes and embryos), Storage: Room temperature Cryovial: storage canes Cryogenic Vial Storage Canes provide the necessary cryogenic storage needed, with a working length of 28.8cm (11.321ft). They allow the transporting and storing vials and cyrosafe tubes holding in cryoca. Storage: Room temperature Cryolocks: Embryosafe: semen x detergents IUI catheter 1ml: B. D. syringe polystyrene. Transfer pipette 3ml. polystyrene. Semen container polystyrene. Storage: Room temp Transfer pipette (3ml) storage: Room temp Centrifuge conical tube 14ml sterile Latex surgical gloves: (Powder free) EVA Biodegradable gloves In line filter: To check contaminants of volatile organic compound and chemical active contaminant. To use inline between incubator and gas sources such as CO2, O2, N2 etc. To be composed with HEPA and especially for active carbon filters. Activated carbon to be powdered and layered to present larger surface area and less air gap. Room temperature storage
