Supply Of Genomic Dna Extraction Mini Kit, Faststart Sybr Green Master, Transcriptor High Fidelity Cdna Synthesis Kit , Genomic Dna Extraction Mini Kit.Format Microcentrifuge Spin Column Isolation Technology Silica Spin Column Sample Type Bacteria, Blood, Cells, Tissue For Use With ( Application ) Genotyping, Real-Time Quantitative Pcr ( Qpcr ) , Southern Blotting, Sequencing, Pcr No. Of Reactions 50 Preps Prep Scale 100 ?L - 1 Ml Blood Samples ( Medium-Scale ) Dna Yield Should Be Atleast 25 Ug Per Ml Of Blood. , Faststart Sybr Green Master, 5 Ml ( 4 × 1.25 Ml; 500 × 20 Ul Reactions ) . Kit Should Contain Faststart Taq Dna Polymerase For Hot-Start Pcr Ready-To-Use, 2× Concentrated Master Mix That Contains All The Reagents ( Except Primers And Template ) Needed For Running Quantitative, Real-Time Dna Detection Assays, Including Qpcr And Two-Step Qrt-Pcr, In The Sybr Green I Detection Format. Addition Of Mgcl2 Not Necessary. Rox Dye Should Be Excluded From Master Mix. The Mix Should Amplify Targets Up To 500Bp. The Mix Should Contain Dutp, To Be Used With Uracil-Dna Glycosylase To Prevent False Positives. Storage At -15 To -25°C For Long Term And At +2 To +8°C For Upto 3 Months. , Transcriptor High Fidelity Cdna Synthesis Kit ( 200Reactions ) Kit Should Include The Following: Transcriptor High Fidelity Reverse Transcriptase ( 1 Vial, 220Ul In Storage Buffer With 200Mm Potassium Phosphate, 2Mmdtt, 0.2%Triton-X100, 50%Glycerol, Ph 7.2 ) , 5X Reaction Buffer ( 250Mmtris / Hcl, 150Mm Kcl, 40Mm Mgcl2, Ph 8.5, 2Vials Each 1Ml ) , Protector Rnase Inhibitor ( 2 Vials Each 50Ul, 40U / Ul, In Storage Buffer: 20Mm Hepes-Koh, 50Mm Kcl, 8Mm Dtt, 50%Glycerol, Ph 7.6 ) , Dntp Mix ( 2 Vials, 200Ul, 10Mm Each ) , Pcr Grade, Anchored-Oligo ( Dt ) 18Primer ( 2 Vials, Each 200Ul, 50Um ) , Random Hexamer Primer ( 2Vials, Each 200Ul, 600Um ) , Dtt ( 1 Vial, 1Ml, 0.1M ) , Water Pcr Grade ( 3 Vials, 1Ml Each ) . Reverse Transcriptase Enzyme Should Transcribe Templates Up To 14 Kb At Temperatures Up To +55°C. Enzyme Blend Should Have 7-Fold Higher Fidelity Compared To Other Commonly Used Reverse Transcriptases. Fidelity Data Should Be Determined By The Sequencing Of Several Million Bases With The Genome Sequencer 20 System. Storage -20Oc
