Tender For Supply Of Lab Chemicals - Ni-Nta, Ampicillin, Iptg, 100Bp Ladder - 1 Ni-Nta Agarose Slurry ( 25 Ml ) Nickel Conjugated Nitrilo Triacetate ( Ni-Nta ) Agarose Affinity Chromatography Matrix For Purifying Recombinant Proteins Carrying A His Tag. Ni-Nta Should Be Coupled To Sepharose Cl-6B For High Affinity Alongwith High Binding Capacity And Low Non-Specific Binding And Support One Step Purification Of His-Tagged Proteins Under Native Conditions From Crude Lysates To ˃95% Purity. Additionally, The Matrix Should Support Protein Purification Under Denaturing Conditions Also. The Matrix Should Allow For Use Of Strong Denaturants And Detergents Including But Not Limited To 6 M Gu·Hcl, 2% Triton X-100, 20 Mm Β-Me, 50% Glycerol, 4 M Mgcl2, Up To 30% Ethanol, 8 M Urea, 2% Tween 20, 10 Mm Dtt, 20% Ethanol, 5 Mm Cacl2, 100 Mm Naoh, 1% Chaps, 20 Mm Tcep, 20 Mm Imidazole, 2 M Nacl, Etc. ) For Efficient Solubilisation And Purification Of Receptors, Membrane Proteins, And Proteins That Form Inclusion Bodies So Also Allow Use Of Reagents Involved In Efficient Removal Of Non-Specifically Binding Contaminants. Matrix Should Elute Proteins Under 100-250 Mm Imidazole Or Change In Ph. The Matrix Should Be Pre-Charged And Ready To Use For Any Scale Of Application. The Matrix Should Purify Proteins For Downscale Applications Such As Structural And Functional Investigations, Crystallization For Determination Of Three-Dimensional Structure, Protein–Protein And Protein–Dna Interaction Assays, Immunization To Produce Antibodies. The Matrix Should Have A Bead Size 45–165 Μm, Binding Capacity Up To 50 Mg / Ml And A Yield Of 100 Μg To 100 Mg 2 Ampicillin Sodium Salt ( 25G ) D- ( − ) -Α-Aminobenzylpenicillin Sodium Salt, Empirical Formula-C16h18n3nao4s, Molecular Weight-371.39, Of Quality Level 200, White To Off White In Colour, Having Melting Point -215°C, Water Solubility At 50 Mg / Ml And Storage At 2-8°C. Expiration Period 3 Years 3 Iptg Dioxane Free ( 5G ) Isopropylthiogalactoside, Isopropyl-Beta-D-Thiogalactopyranoside, Formula C9h18o5s, Molecular Weight 238.3, Purity ≥ 99.0% Confirmed By Hplc 4 100Bp Dna Ladder Ready To Use ( 50Ug ) The 100 Bp Dna Ladder Should Contain 10 Bands Of Double Stranded Linear Dna Fragments ( Viz., 100 Bp, 200 Bp, 300 Bp, 400 Bp, 500 Bp, 600 Bp, 700 Bp, 800 Bp, 900 Bp, 1000 Bp ) And Should Be Suitable For Sizing Of Dna Fragments Generated By Pcr Or Restriction Digestion, Genomic Dna Fragments, Etc., Separated On Agarose Gel. The 600 Bp And 1000 Bp Bands Should Be Spiked As Reference Point For Easy Detection. The Dna Ladder Must Be Supplied In 10 Mm Tris-Hcl ( Ph 8.0 ) And 10 Mm Edta And Should Have A Storage At -20°C
