Tender For Oligonucleotides, trombay-Maharashtra

Tender For Oligonucleotides Synthesis And Supply Of Custom Oligonucleotides With Detailed Specifications As Per Boq , 1. Synthesis And Supply Of Custom Oligonucleotides A. Total Number Of Nucleotide Bases To Be Custom Synthesized Is 3000 Bases. B. Sequences Of The Oligonucleotides To Be Synthesized Will Be Provided By The User. C. The Scale Of Synthesis Should Be At Least 0.05 ?Mol, Qc: Ms / Maldi-Tof Purification: Desalted D. Oligonucleotide Synthesis Report Should Be Provided Along With The Synthesized Samples E. The Bidder Shall Quote For The Fabrication Of These Components Without Material Supply. F. The Bidder Shall Deliver The Oligonucleotides To Barc In Lyophilized Form, Along With The Details Characteristics Of All The Oligonucleotides As A Hard Copy Report. , 2. Synthesis And Supply Of Custom Fluorescence Tagged Oligonucleotides- 50 Mer A. Total Number Of Nucleotide Bases To Be Custom Synthesized Under This Item Is 250 Bases. B. Sequences Of The Oligonucleotides To Be Synthesized Will Be Provided By The User. C. The Scale Of Synthesis Should Be At Least 100 Nmol, Qc: Ms / Maldi-Tof Purification: Desalted D. Hplc Purification And Validation. E. Oligonucleotide Synthesis Report Should Be Provided Along With The Synthesized Samples F. The Bidder Shall Deliver The Oligonucleotides To Barc In Lyophilized From Along With The Details And Characteristics Of All The Oligonucleotides As A Hard Copy Report. G. Fluorescent Dyes To Be Labelled Would Be One Of The Following- Rox, Tamra, Fam, Hex, Alexa Dyes H. Vendor Should Quote The Amount Taking Into Consideration All The Above Mentioned Dyes And Not One Single Dye. I. At Least 5 Labelling Reactions At One End ( 5` Or 3` ) ( I.E 20-80 Mer Tagged With Fluorescent Dye * 5 Nos ) Should Be Performed As Per The Sequences Provided And Supplied. ( 1 Set= 50 Mer * 1 Labelling Reaction ) , 3. Synthesis And Supply Of Custom Fluorescence Quencher Tagged Oligonucleotides- 50 Mer A. Total Number Of Nucleotide Bases To Be Custom Synthesized Under This Item Is 250 Bases. B. Sequences Of The Oligonucleotides To Be Synthesized Will Be Provided By The User. C. The Scale Of Synthesis Should Be At Least 100 Nmol, Qc: Ms / Maldi-Tof Purification: Desalted D. Hplc Purification And Validation. E. Oligonucleotide Synthesis Report Should Be Provided Along With The Synthesized Samples F. The Bidder Shall Deliver The Oligonucleotides To Barc In Lyophilized From Along With The Details And Characteristics Of All The Oligonucleotides As A Hard Copy Report. G. Fluorescence Quencher To Be Tagged Would Be One Of The Following- Bhq1, Bhq2, Bbq H. At Least 5 Labelling Reactions At One End ( 5` Or 3` ) ( I.E 20-80 Mer Tagged With Fluorescence Quencher * 5 Nos ) Should Be Performed As Per The Sequences Provided And Supplied. ( 1 Set= 50 Mer * 1 Labelling Reaction ) , 4. Synthesis And Supply Of Custom Biotin / Dig Tagged Oligonucleotides- 50 Mer A. Total Number Of Nucleotide Bases To Be Custom Synthesized Under This Item Is 250 Bases. B. Sequences Of The Oligonucleotides To Be Synthesized Will Be Provided By The User. C. The Scale Of Synthesis Should Be At Least 100 Nmol, Qc: Ms / Maldi-Tof Purification: Desalted D. Hplc Purification And Validation. E. Oligonucleotide Synthesis Report Should Be Provided Along With The Synthesized Samples F. The Bidder Shall Deliver The Oligonucleotides To Barc In Lyophilized From Along With The Details And Characteristics Of All The Oligonucleotides As A Hard Copy Report. G. Tags Include One Of The Following- Dig Or Biotin H. At Least 5 Labelling Reactions At One End ( 5` Or 3` ) ( I.E 20-80 Mer Tagged With Dig / Biotin Dye * 5 Nos ) Should Be Performed As Per The Sequences Provided And Supplied. ( 1 Set= 50 Mer * 1 Labelling Reaction ) , 5. Synthesis And Supply Of Custom Oligos With 2-Amino Purine Modifications ( Internal ) A. Total Number Of Nucleotide Bases To Be Custom Synthesized Under This Item Is 250 Bases. B. Sequences Of The Oligonucleotides To Be Synthesized Will Be Provided By The User. C. The Scale Of Synthesis Should Be At Least 100 Nmol, Qc: Ms / Maldi-Tof Purification: Desalted D. Hplc Purification And Validation. E. Oligonucleotide Synthesis Report Should Be Provided Along With The Synthesized Samples F. The Bidder Shall Deliver The Oligonucleotides To Barc In Lyophilized From Along With The Details And Characteristics Of All The Oligonucleotides As A Hard Copy Report. G. Internal Modifications Include - 2 Amino Purine H. At Least Labelling 5 Reactions ( I.E 20-80 Mer With Internal 2-Aminopurine Modifications Should Be Performed To The Adenine As Per The Sequences Provided And Supplied. ( 1 Set= 50 Mer * 1 Internal Modification Reaction )

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