|
| 1 | Metabolomics Studies |
| 2 | Method Development for Metabolomics Studies - (One Time Charges Per Sample/Project) |
| 3 | Metabolite Extraction from tissue, leaf, root and other sample types |
| 4 | Targeted Metabolomics Studies using LCMS - MS with Data Analysis |
| 5 | Untargeted Metabolomics Studies using LCMS - MS with Data Analysis |
| 6 | Targeted lipidomics using LCMS - MS |
| 7 | Metabolic Flux Analysis using LCMS - MS |
| 8 | Gas Chromatography Mass Spectrometry GC-MS |
| 9 | Nuclear magnetic resonance spectroscopy NMR |
| 10 | MS Mass Spectrometry |
| 11 | High Liquid Performance Chromatography HPLC Analysis |
| 12 | Proteomics Studies |
| 13 | Protein Extraction from tissue, leaf, root, and other sample types |
| 14 | Protein Identification & Analysis (wet-lab and in-silico bioinformatics work) |
| 15 | Protein-Protein Interaction Network Analysis & Identification (in-silico bioinformatics work) |
| 16 | Protein Post-translational Modification Analysis (in-silico bioinformatics work) |
| 17 | MALDI-MS |
| 18 | LC-MS (Q-TOF) |
| 19 | Reverse-Phase Liquid Chromatography RP-HPLC |
| 20 | Protein Gel electrophoresis & Imaging |
| 21 | Amino Acids Profiling |
| 22 | Surface Plasmon Resonance (SPR) Technology |
| 23 | Electrospray Ionisation with Tandem Mass Spectrometry (ES-MS/MS) |
| 24 | SDS-PAGE electrophoresis (along with sample preparation) |
| 25 | Nano Liquid Chromatography with Tandem Mass Spectrometry Detection (LC-MS/MS) |
| 26 | Flow cytometry Studies |
| 27 | Genome size estimation / ploidy level detection |
| 28 | Fluorescence in situ hybridization (FISH) |
| 29 | Immuno phenotyping |
| 30 | Green fluorescent protein (GFP) tagging |
| 31 | Chromosomal integration of GFP |
| 32 | Microscopy/imagery |
| 33 | Scanning electron microscope |
| 34 | Transmission electron microscope |
| 35 | Anatomical assays/ detection |
| 36 | Confocal microscopy |
| 37 | Nanoparticle/nanomaterial electron microscopy |
| 38 | Energy dispersive X Ray Spectrophotometry |
| 39 | Immuno electron microscopy |
| 40 | Ionomics Studies |
| 41 | X-Ray Fluorescence (XRF) |
| 42 | Inductively Coupled Plasma Mass Spectrometry (ICP-MS) |
| 43 | MIDI Sherlock ® MICROBIAL IDENTIFICATION SYSTEM |
| 44 | Isotopes labeling0 systems/ Isotope studies/ carbon and nitrogen etc. |
| 45 | Antibody /specific molecule synthesis |
| 46 | Antibody development (Monoclonal, conjugated IgG antibody for the given locus id, Information of the fluorescent label or enzyme with substrate information and availability with cost, able to use on PAGE or SDS-PAGE or both, Minimum quantity and concentration must be specified |
| 47 | Pesticide (herbicide, fungicide, insecticides etc.) residue analysis |
| 48 | Elemental analysis |
| 49 | Elemental analysis eg: nitrogen content / carbon/phosphorous etc. |
| 50 | Atomic Absorption Spectrophotometer (AAS) |
| 51 | X-ray Diffraction (XRD) analysis |
| 52 | Fourier Transform Infra-Red (FTIR) spectrophotometer |
| 53 | Histidine tagging of microbes |
| 54 | Green fluorescent protein tagging of microbes |
| 55 | Nutrient concentration in Plant sample- N, P, K, S, Fe, Zn, Cu, B and Mn |
| 56 | Nutrient content in soil sample - N, P, K, S, Fe, Zn, Cu, B, Ca, Mg and Mn |
| 57 | Mechanical analysis of soil- Percentage of Sand, Silt and Clay |
| 58 | Greenhouse gas estimation- Methane, Carbon di oxide and Nitrous oxide |
| 59 | Water quality analysis- pH, conductivity, dissolved oxygen and turbidity |
| 60 | Sanger Sequencing, Genotyping, Microarray & Next Generation Sequencing Services |
| 61 | Sanger sequencing of plasmid/ clones/ PCR product/ amplicon up to 800 bp |
| 62 | Sanger sequencing of plasmid/ clones/ PCR product/ amplicon > 800 bp – 1500 bp |
| 63 | Sanger sequencing of plasmid/ clones/ PCR product/ amplicon > 1500 bp – 3000 bp |
| 64 | Genotyping-by-sequencing GBS - 100,000 raw tags per sample |
| 65 | Genotyping-by-sequencing GBS - 200,000 raw tags per sample |
| 66 | Genotyping-by-sequencing GBS - 300,000 raw tags per sample |
| 67 | Microarray Affymetrix (Rice Genome Array/ custom arrays) |
| 68 | Microarray Agilent (gene expression/ exon microarrays/ Rice Gene Expression Microarrays)1 K RiCA |
| 69 | Microarray Agilent (gene expression/ exon microarrays/ Rice Gene Expression Microarrays)7 K SNP |
| 70 | Microarray Agilent (gene expression/ exon microarrays/ Rice Gene Expression Microarrays) 50 K SNP |
| 71 | Targeted enrichment and re-sequencing |
| 72 | 5 kb - 10 kb region (10 x coverage) |
| 73 | 5 kb - 10 kb region (25 x coverage) |
| 74 | 5 kb - 10 kb region (50 x coverage) |
| 75 | Amplicon sequencing |
| 76 | Amplicon sequencing (5 rice genes) |
| 77 | Amplicon sequencing (10 rice genes) |
| 78 | Amplicon sequencing (50 rice genes) |
| 79 | Complete genome analysis assembly (resequencing new or pathogen/ pest/ virus lacking a reference genome) (de novo/ref), annotation, comparative analysis, GO analysis, KEGG pathway mapping, target identification, secondary structure, prediction, MapMan analysis, QTL mapping, SNP variant calling, graphical representation of the data , visualization of data, any specific analysis need based as intended by indentor. |
| 80 | Miseq (2x150bp), at least 6 GB raw data with Q30 data |
| 81 | NovaSeq 6000 or Higher Illumina Platform (2x150bp), output at least 10 GB raw data |
| 82 | NovaSeq 6000 or Higher Illumina Platform (2x150bp), output at least 20 GB raw data |
| 83 | NovaSeq 6000 or Higher Illumina Platform (2x150bp), output at least 30 GB raw data |
| 84 | NovaSeq 6000 or Higher Illumina Platform (2x150bp), output at least 40 GB raw data |
| 85 | NovaSeq 6000 or Higher Illumina Platform (2x150bp), output at least 50 GB raw data |
| 86 | One SMRT of PacBio Sequel, 50 Gb raw data, >10 Kb read length Complete library preparation, whole genome / RNA-seq complete analysis |
| 87 | One SMRT of PacBio Sequel Systems, read lengths>10Kb and up to 80 GB raw data |
| 88 | One sample on Oxford nanopore, up to 200Mb raw data |
| 89 | One sample on Oxford PromethION, up to 30 GB raw data |
| 90 | Small RNA sequencing, 1x50bp, at least 25 million reads, Q30 values |
| 91 | Transcriptome sequencing, 2x150bp, at least 20 million raw reads - Library preparation Complete transcriptome data analysis - (including assembly (de novo or reference-based), annotation , Differential expression, comparative analysis, alternate splice variants, GO analysis, KEGG mapping, etc) |
| 92 | Transcriptome sequencing, 2x150bp, atleast 30 million raw reads - Library preparation Complete transcriptome data analysis - (including assembly (de novo or reference-based), annotation , Differential expression, comparative analysis, alternate splice variants, GO analysis, KEGG mapping, etc) |
| 93 | Transcriptome sequencing, 2x150bp, atleast 40 million raw reads - Library preparation Complete transcriptome data analysis - (including assembly (de novo or reference based), annotation , Differential expression, comparative analysis, alternate splice variants, GO analysis, KEGG mapping, etc) |
| 94 | Transcriptome sequencing, 2x150bp, atleast 50 million raw reads - Library preparation Complete transcriptome data analysis - (including assembly (de novo or reference based), annotation , Differential expression, comparative analysis, alternate splice variants, GO analysis, KEGG mapping, etc) |
| 95 | Transcriptome sequencing -Small RNA library preparation Complete small RNA analysis assembly (de novo/ref),annotation, differential expression, comparative analysis, GO analysis, KEGG pathway mapping, target identification, secondary structure, prediction, MapMan analysis, QTL mapping, SNP variant calling, any specific analysis need based as intended by indentor. |
| 96 | Iso Seq library prep and sequencing, atleast 10 GB raw data per library - Library preparation (including assembly (de novo or reference-based), annotation , Differential expression, comparative analysis, alternate splice variants, GO analysis, KEGG mapping, etc) |
| 97 | DNA isolation, purification, bisulfate conversion for methylome analysis, quality check Complete bisulfite data analysis including scanning/distribution, GO analysis, methylated region/gene/promoter identification, plotting, graphs, comparative mapping, visualization methods, any specific analysis need based as intended by intender |
| 98 | Metagenomics - Library preparation Data Analysis of Metagenomics sequencing, identification of organisms , Classification and diversity analysis |
| 99 | Degradome sequencing - Library preparation |
| 100 | RAD sequencing per sample, at least 2GB raw data- Library preparation |
| 101 | Genotyping cost of samples for 5000-60000 SNPs, 96 samples |
| 102 | Genotyping cost of sample for 5000-60000 SNPs,192 samples |
| 103 | Genotyping cost of sample for 5000-60000 SNPs,384 samples |
| 104 | qRT-PCR by SYBR green/sample |
| 105 | KASP assays for SNP genotyping |
| 106 | Gene Chip arrays for plants/sample |
| 107 | Vector construction for CRISPR |
| 108 | Data Analysis of Resequencing/ whole-genome data and Mut-map analysis, SNP analysis, SNPs identification, SNP annotation |
| 109 | Data Analysis of resequencing, whole-genome analysis and QTL-seq analysis, QTL detection, SNP analysis, SNPs identification, SNP annotation 1 |
| 110 | GBS Data Analysis , SNP calling, Map Creation |
| 111 | Exome sequencing data analysis including assembly, annotation, variant analysis, any specific analysis need based as intended by intender etc. |
| 112 | Vector designing/ construction and synthesis |
| 113 | Next-generation sequencing library preparation for DNA |
| 114 | For Illumina |
| 115 | For SMRT PacBio |
| 116 | For Oxford Nanopore |
| 117 | Next-generation sequencing library preparation for RNA |
| 118 | For Illumina |
| 119 | For SMRT PacBio |
| 120 | For Oxford Nanopore |
| 121 | Next-generation sequencing library preparation for small RNA |
| 122 | For Illumina |
| 123 | For SMRT PacBio |
| 124 | For Oxford Nanopore |
