Tender For Supply Of Consumables Human DNA Inclusive of Bisulfite Modification and Data analysis. Target regions- promoter region CpGs for OPRM1,DAT1,DRD2 and DRD4 genes Taqman Universal Master mix (10ml) Specifications: • AmpliTaq Gold DNA Polymerase, UP (Ultra Pure) • dNTPs (with dUTP) • ROX Passive Reference • Optimized buffer components • TaqMan gene expression assays • TaqMan microRNA assays • TaqMan drug metabolism genotyping assays • TaqMan SNP genotyping Assays. 50bp DNA ladder Specifications: Compatible for Agarose or polyacrylamide gels. Visualized after ethidium bromide or SYBR green staining. For determination of size and quantification of DNA fragments. 1Kb DNA ladder Specifications: Compatible for Agarose or polyacrylamide gels. Visualized after ethidium bromide or SYBR green staining. For determination of size and quantification of DNA fragments. 50mL Measuring Cylinder Specifications: Autoclavable, chemical resistance. polymethylpentene cylinder Hexagonally-shape bases. Vertical Freezer Rack for Cryocube Box(8 Places) Specifications: Stainless. Withstand upto -800C. Hand Protector Grip Specifications: Heat Resistance. Thumb and Fingers fit. Made of silicon rubber. Non slip gripping surface DNA Isolation Mini Kit ( 250 reaction) Specifications: Application: PCR Southern blotting Elution volume: 50-200μl Main sample type: Whole blood, tissue, cells. Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein: Genomic DNA, mitochondrial DNA, bacterial DNA, Parasite DNA, viral DNA. Sample amount: 200μl/25mg/5X 10e6 Yield: 4-30μg Compatible with Qiacube 9. Leupeptin(5mg)L2884-5MG Molecular Biology grade 10. Methylation Specific PCR(MSP) Kit ( 200 reaction) Specifications: Applications: Highly reliable and accurate methylation-specific PCR Enzyme activity: 5’->3’ exonuclease activity Reaction Type: PCR amplification Master Mix: Yes 11. 50mL Centrifuge Tube(sterile) (500/Pkt) Specifications: Temperature range from -800C to 1200C, 5 years shelf life Leak proof design, easy on/easy off cap, clear accurate graduations Certified RNase /DNase free, heavy metal free color concentrate, medical grade polypropylene, nonpyrogenic sterile 12. PCR Tubes, 0.2mL(1000) Specifications: Withstand +1210C to -800C . Certified RNase /DNase free, heavy metal free, medical grade polypropylene, nonpyrogenic. Flat Cap. 13. Rotor Adapters(10×24) Specifications: Sterile, Disposable. Compatible with QIAcube. 14. Filter Tips 1000 μL(1024) Specifications: Disposable Filter-Tips, racked: (8×128) For use with the QIAcube instrument. 15. Spinwin Tube Conical Bottom(15ml)(500 /pkt) Specifications: Temperature range from -800C to 1200C, 5 years shelf life Leak proof design, easy on/easy off cap, clear accurate graduations Certified RNase /DNase free, heavy metal free color concentrate, medical grade polypropylene, nonpyrogenic sterile 16. 0.1ml 4-Tubes & Cap Strips(250/pkt) Specifications: Compatible with Rotor Gene Q 17. 1.5 ml Centrifuge tube(500/pkt) Specifications: Free from natural rubber and heavy metal Capable to withstand upto 20000 RCF Autoclavable, DNase/RNase and pyrogen free Click seal graduated 18. GLOVES – SMALL(100/pkt) Specifications: Powder free disposable gloves to protect the hand from all types of hazard- chemicals particulates, biological and other contaminants. Puncture and chemicals resistance Without the presence of any natural rubber proteins. 19. GLOVES- MEDIUM(100/pkt) Without the presence of any natural rubber proteins. 20. Micro TIPS (200-1000 μL)(500/pkt) Specifications: USP class 6, FDA approved medical grade, bovine free, and heavy metal free, and free plastics resins. 21. Micro Tips (0.2-10 μL)(1000/pkt) Specifications: USP class 6, FDA approved medical grade, bovine free, and heavy metal free, and free plastics resins. 22. Micro Tips (2-200 μL)(1000/pkt) Specifications: USP class 6, FDA approved medical grade, bovine free, heavy metal free, and free plastics resins. 23. 0.2 ml PCR Rack with cover(6 /pkt) Specifications: Withstand upto -800C 24. Filter Tips 200 μL(1024) Specifications: Disposable Filter-Tips, racked: (8×128) For use with the QIAcube instrument 25. 1.5 ml Centrifuge tube (Amber)( 500/pkt) Specifications: Withstand +1210C to -800C and RCF 27,000g, click seal graduated. 26. 2ml Centrifuge Tube(Amber)(500/pkt) Specifications: Withstand +1210C to -800C and RCF 27,000g, click seal graduated. 27. 0.5ml Centrifuge Tube(Amber) (500 /pkt) Specifications: Withstand +1210C to -800C and RCF 27,000g, click seal graduated. 28. 0.5ml Centrifuge Tube (500/pkt) Specifications: Withstand +1210C to -800C and RCF 27,000g, click seal graduated. 29. Parafilm(2’’x 250’) Specifications: Semi-transparent, flexible and water proof. Resistant to acids and alchohol. 30. RNase Free Water(12×1.9ml) Specifications: RNase, DNase, Protease and diethylpyrocarbonate free. Suitable for all types of PCR. Molecular Biology Grade
