|
| 1 | Purchase of chemicals and reagents |
| 2 | RAW 264.7 Cell |
| 3 | HEK-293 |
| 4 | INS-1 832/3 Rat Insulinoma Cell Line |
| 5 | Dulbecco\"s Modified Eagle\"s Medium (DMEM) |
| 6 | DMEM, high glucose, GlutaMAX™ Supplement |
| 7 | RPMI Medium 1640 (1X), liquid; with L-glutamine |
| 8 | RPMI 1640 Medium, no glucose |
| 9 | Fetal Bovine Serum, qualified, USDA-approved regions |
| 10 | Penicillin-Streptomycin(10,000 U/mL) |
| 11 | HEPES sodium salt |
| 12 | PYRUVIC ACID, Sodium Salt, Reagent Grade |
| 13 | 2-Mercaptoethanol |
| 14 | Trypsin-EDTA (0.05%), phenol red |
| 15 | Normal Goat Serum (10%) |
| 16 | Normal Goat Serum (10%) |
| 17 | Syntaxin 2 shRNA Plasmid (m) |
| 18 | Mouse STX2 shRNA silencing Adenovirus/Ad-m-STX2-shRNA-mCherry/RFP/CFP |
| 19 | Lipofectamine™ 2000 Transfection Reagent |
| 20 | Opti-MEM™ I Reduced Serum Medium, no phenol red |
| 21 | Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit |
| 22 | Premo™ Autophagy Sensor LC3B-GFP (BacMam 2.0) |
| 23 | Zymosan A BioParticles, Alexa Fluor 594-conjugated |
| 24 | Zymosan A BioParticles opsonizing reagent |
| 25 | pHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis |
| 26 | Fc OxyBURST™ Green Assay Reagent |
| 27 | Diabetes Associated Peptide Amide human |
| 28 | ProLong™ Diamond AntifadeMountant with DAPI |
| 29 | LC3A/B (D3U4C) XP® Rabbit mAb #12741 |
| 30 | Atg16L1 (D6D5) Rabbit mAb #8089 |
| 31 | PI3 Kinase Class III (D9A5) Rabbit mAb #4263 (Rabbit) |
| 32 | Beclin-1 Complex Antibody Sampler Kit #59187 |
| 33 | Anti-mouse IgG, HRP-linked Antibody #7076 |
| 34 | Anti-rabbit IgG, HRP-linked Antibody #7074 |
| 35 | Normal Rabbit IgG #2729 |
| 36 | Super Signal™ West Pico PLUS Chemiluminescent Substrate |
| 37 | Protein A - Sepharose™ 4B |
| 38 | Syntaxin 2 Polyclonal Antibody (Rabbit Polyclonal) |
| 39 | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
| 40 | Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) |
| 41 | Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt) |
| 42 | Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) |
| 43 | Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt) |
| 44 | Alexa Fluor® 647 AffiniPure Goat Anti-Rabbit IgG (H+L) |
| 45 | Alexa Fluor® 647 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat SrProt) |
| 46 | Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) |
| 47 | Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, SwSrProt) |
| 48 | Alexa Fluor® 594 AffiniPure Goat Anti-Mouse IgG (H+L) |
| 49 | Alexa Fluor® 594 AffiniPure Goat Anti-Mouse IgG (H+L)(min X Hu, Bov, Hrs, Rb, SwSrProt) |
| 50 | Alexa Fluor® 647 AffiniPure Goat Anti-Mouse IgG (H+L) |
| 51 | Alexa Fluor® 647 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, SwSrProt) |
| 52 | D-GLUCOSE, Anhydrous, Reagent Grade |
| 53 | Dextrose extrapure AR, ACS |
| 54 | Sodium Chloride for molecular biology, 99.9% |
| 55 | Potassium Chloride (KCl) |
| 56 | Disodium phosphate (Na2HPO4) |
| 57 | Monopotassium phosphate (KH2PO4) |
| 58 | Potassium hydroxide (KOH) |
| 59 | Sodium Hydroxide 1N Solution |
| 60 | TRITON X-100, Biotechnology Grade |
| 61 | Protease Inhibitor Cocktail |
| 62 | Halt™ Protease Inhibitor Cocktail, EDTA-free (100X) |
| 63 | SODIUM DODECYL SULFATE Reagent Grade : Purity: >99% |
| 64 | Sodium Lauryl Sulphateextrapure AR, ACS, 99% |
| 65 | Trizma® base |
| 66 | Tris Buffer Superior extrapure AR, ACS, 99.9% |
| 67 | GLYCINE, Reagent Grade, min 99% Suitable for Electrophoresis |
| 68 | Glycine extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5% |
| 69 | EDTA Disodium Salt 0.5M Solution |
| 70 | Glycerol for molecular biology, 99.5% |
| 71 | Methanol |
| 72 | Hydrochloric Acid 1N Aq. Solution |
| 73 | Acrylamide/Bis-acrylamide, 30% solution |
| 74 | ACRYLAMIDE/BIS-ACRYLAMIDE, 30% Solution 19:1 |
| 75 | TEMED, Electrophoresis Grade |
| 76 | Sodium palmitate |
| 77 | Sodium oleate |
| 78 | ALBUMIN, BOVINE SERUM , Heat Shock Isolation, Fraction V. Min. 98% |
| 79 | Albumin Bovine (pH 5.2) fraction V (Bovine Serum Albumin, BSA), 98% |
| 80 | Sodium hypochlorite |
| 81 | Propionaldehyde |
| 82 | Sodium fluoride |
| 83 | Sodium thiocyanate |
| 84 | Ammonium hydroxide |
| 85 | 2-4-Dichlorophenoxyacetic acid |
| 86 | Diethylene Glycol Monoethyl Ether |
| 87 | Low melting point Agarose |
| 88 | Agarose |
| 89 | Sodium dodecyl sulfate (SDS) |
| 90 | Triton X-100 |
| 91 | Ethidium Bromide |
| 92 | Sodium Carbonate Anhydrous extrapure AR, 99.9% |
| 93 | L-Alanine extrapure CHR, 99% |
| 94 | Trichloroacetic Acid extrapure, 99% |
| 95 | Glutathione Reduced (GSH) ExiPlus, 99% |
| 96 | Tetrasodium Pyrophosphate Decahydrate (TSPP Decahyd) extrapure AR, 99% |
| 97 | PhenazoniumMethosulphate (PMS) extrapure, 99% |
| 98 | Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) extrapure AR, 99% |
| 99 | ß-Nicotinamide Adenine Dinucleotide Disodium Salt (Reduced) (ß-NADH.Na2, DPNH.Na2) extrapure, 98% |
| 100 | Acetic Acid Glacial extrapure AR, 99.9% |
| 101 | n-Butyl Alcohol pure, 99% |
| 102 | Potassium Phosphate Dibasic Anhydrous pure, 99% |
| 103 | Potassium Dihydrogen Orthophosphate extrapure AR, 99.5% |
| 104 | tert-Butyl hydroperoxide |
| 105 | Bisbenzimide (Hoechst 33258) |
| 106 | L-Glutathione reduced |
| 107 | 1-CHLORO-2,4-DINITROBENZENE |
| 108 | 2-Thiobarbituric acid, Hi-AR™ |
| 109 | 5,5’-Dithiobis(2-nitrobenzoic acid) |
| 110 | Sodium Bicarbonate |
| 111 | Sodium hypochlorite (4% w/v solution) |
| 112 | Brij-30 (Polyethylene Glycol Dodecyl Ether, Brij-L4) |
| 113 | Sodium Lauryl Ether Sulphate (SLES, AES), 28-30% |
| 114 | Zinc Chloride pure, 97% |
| 115 | Chromium (VI) Oxide pure (Chromium Trioxide), 99% |
| 116 | Nickel (II) Oxide pure, 77% Ni |
| 117 | Lead oxide, red |
| 118 | Mercuric Chloride extrapure AR, ACS, ExiPlus, 98% |
| 119 | Sodium Lauryl Sulphateextrapure AR, ACS, 99% |
| 120 | Methylparaben |
| 121 | Ethylparaben |
| 122 | PFOA-Perfluorooctanoic acid |
| 123 | Glutathione Reductase |
| 124 | Anthracene |
| 125 | Tris Buffer |
| 126 | Napthalene |
| 127 | Anthracene |
| 128 | Phenanthrene |
| 129 | Pyrene |
| 130 | Acenaphthene |
| 131 | Fluorene |
| 132 | Sodium dodecylbenzene sulfonate |
| 133 | Transformation aid kit |
| 134 | Plasmid Isolation Mini Kit |
| 135 | Agarose Gel Extraction Kit |
| 136 | Genomic DNA Isolation Kit |
| 137 | dNTP Mix for PCR |
| 138 | Pfu DNA Polymerase |
