Eastern Railways-ER has published Supply Of Dengue Ns-1 Ag Elisa Test Kit, Pack Of 48 Tests Eachspecification:1. The Elisa Kit Should Be Designed For Qualitative Detection Of Dengue Ns1 Antigen Of All 4 Dengue Serotypes In Human Serum.2. The Kit Should Be Provided With The Following Mate. Submission Date for this Tender is 18-03-2021. Food and Drug Additive Tenders in West Bengal. Bidders can get complete Tender details and download the document.
Sl. No. | Item Description |
1 | Dengue NS-1 Ag Elisa Test Kit, Pack of 48 Tests each Specification: 1. The ELISA kit should be designed for qualitative detection of dengue NS1 antigen of all 4 dengue serotypes in human serum. 2. The kit should be provided with the following materials and reagents: a. Anti-NS1 Antibody Coated Breakway Microwells. Desiccant should be provided for storing the unused microwells which are to be resealed immediately. b. Horseradish peroxidase conjugated Anti-NS1 monoclonal antibody with preservatives. c. Chromogenic substrate in buffer. d. Positive Control in the form of recombinant antigen with preservatives and antibiotics. e. Negative control in the form of confirmed negative human serum with preservatives and antibiotics. f. Calibrators in the form of recombinant antigen with preservatives and antibiotics. g. Sample diluents. h. Wash Buffer. 3. The time required for performing the test for detection of dengue NS1 antigen by ELISA should range between 2-4 hours. 4. The ELISA kit for detection of dengue NS1 antigen should have a sensitivity of >90% and a specificity of >95% taking RT-PCR as the gold standard. 5. The kit should have a shelf life of at least 18 months when stored at an ambient temperature of 2 degree C to 8 degree C. 6. Transportation should be under cold chain. 7. Demonstration should be done to the satisfaction of the user department. |
2 | Dengue NS-1 Ag Elisa Test Kit, Pack of 48 Tests each Specification: 1. The ELISA kit should be designed for qualitative detection of dengue NS1 antigen of all 4 dengue serotypes in human serum. 2. The kit should be provided with the following materials and reagents: a. Anti-NS1 Antibody Coated Breakway Microwells. Desiccant should be provided for storing the unused microwells which are to be resealed immediately. b. Horseradish peroxidase conjugated Anti-NS1 monoclonal antibody with preservatives. c. Chromogenic substrate in buffer. d. Positive Control in the form of recombinant antigen with preservatives and antibiotics. e. Negative control in the form of confirmed negative human serum with preservatives and antibiotics. f. Calibrators in the form of recombinant antigen with preservatives and antibiotics. g. Sample diluents. h. Wash Buffer. 3. The time required for performing the test for detection of dengue NS1 antigen by ELISA should range between 2-4 hours. 4. The ELISA kit for detection of dengue NS1 antigen should have a sensitivity of >90% and a specificity of >95% taking RT-PCR as the gold standard. 5. The kit should have a shelf life of at least 18 months when stored at an ambient temperature of 2 degree C to 8 degree C. 6. Transportation should be under cold chain. 7. Demonstration should be done to the satisfaction of the user department. |
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