Tender For Supply Of Kit And Chemical For New Strain Of Corona Viurs , Department Of Microbiology Kgmu Up Lucknow, KGMU UP LUCKNOW-Uttar Pradesh

Tender For Supply Of Kit And Chemical For New Strain Of Corona Viurs , Department Of Microbiology Kgmu Up Lucknow-1 Following reagents are required for capillary sequencing on ABI capillary sequencer (Instrument 3500Dx and 3130 sequencer) 2 Master mix for COVID-19 sequencing 3 Gel and PCR purification kit 4 Terminator v3.1 Cycle Sequencing Kit with additional 5X sequencing Buffer (5 per kit): 5 Hi-Di Formamide 5ml 6 POP-7 polymer: 100-sample pouch of POP-7polymer for the 3500/3500xl Genetic Analyzer 7 Cathode Buffer: pack of 4 8 Anode Buffer: Pack of 4 9 SARS COV2 panel – manual 10 Conditioning reagents 11 Following reagents are required for NGS on Oxford Nanopore Technologies sequencer, Promethion 12 Flow Cells (total 16 flow cell) compatible with Promethion (Oxford Nanopore Technologies) sequencer which is required for Whole Genome Sequencing (WGS) of 300 samples (24-multiplexed). 13 Native Barcoding of 1-12 and 13-24 (nuclease free) required for multiplexing and sequencing library preparation, compatible with Promethion sequencer (Oxford Nanopore Technologies). 14 Ligation Sequencing Kit compatible with Promethion sequencer’s (Oxford Nanopore Technologies) WGS library preparation for sequencing. 15 Flow Cell Priming Kit for priming flow cells of Promethion (Oxford Nanopore Technologies). 16 Hot Start High-Fidelity Master mix for cDNA amplification compatible with Promethion (Oxford Nanopore Technologies) sequencer 17 Random Primer Mix required for cDNA synthesis and compatible for sequencing library preparation to be sequenced on Promethion sequencer (Oxford Nanopore Technologies). 18 End Repair/dA-Tailing Module is required for cDNA end repairing which is compatible with Promethion (Oxford Nanopore Technologies) library preparation 19 Ligation Module required for sequencing samples barcoding compatible for library preparation to be sequenced on Promethion (Oxford Nanopore Technologies). 20 Deoxynucleotide (dNTP) Solution Mix 10mM 21 Quick Ligation module is required for adapter ligation for sequencing and compatible with Promethion sequencer (Oxford Nanopore Technologies). 22 COVID-19 primers mix covering the whole genome of SARS CoV2 for whole genome amplification and sequencing. 23 Rnase inhibitior for molecular biology work, especially used in cDNA preparation 24 SuperScript™ IV VILO™ Master Mix required for cDNA synthesis compatible with Promethion (Oxford Nanopore Technologies)library preparation and sequencing. 25 Qubit dsDNA HS assay kit for quantitation of library. 26 Qubit Assay Tubes for library quantitation. 27 Qubit RNA ASSAY KIT required for RNA quantitation. 28 Gel Cassettes for assessing amplicon quality (0.8-1.0 %) 29 Magnetic beads mix (DNase and RNase free) for cDNA and library purification compatible with Promethion (Oxford Nanopore Technologies) library preparation and sequencing.

INR 19116 /-

INR 108500.0 /-

Refer document

Name of Work: < MEDICAL INSTRUMENTS>
	Sl. No.	Item Description	Item Code / Make	Quantity	Units
1	1	Following reagents are required for capillary sequencing on ABI capillary sequencer (Instrument 3500Dx and 3130 sequencer)
2	1.01	Master mix for COVID-19 sequencing	item1	4	Nos
3	1.02	Gel and PCR purification kit	item2	12	Nos
4	1.03	Terminator v3.1 Cycle Sequencing Kit with additional 5X sequencing Buffer (5 per kit):	item3	5	Nos
5	1.04	Hi-Di Formamide 5ml	item4	18	Nos
6	1.05	POP-7 polymer: 100-sample pouch of POP-7polymer for the 3500/3500xl Genetic Analyzer	item5	40	Nos
7	1.06	Cathode Buffer: pack of 4	item6	4	Nos
8	1.07	Anode Buffer: Pack of 4	item7	4	Nos
9	1.08	SARS COV2 panel – manual	item8	2	Nos
10	1.09	Conditioning reagents	item9	4	Nos
11	1.1	Following reagents are required for NGS on Oxford Nanopore Technologies sequencer, Promethion	item10	1	Nos
12	1.11	Flow Cells (total 16 flow cell) compatible with Promethion (Oxford Nanopore Technologies) sequencer which is required for Whole Genome Sequencing (WGS) of 300 samples (24-multiplexed).	item11	300	rxn
13	1.12	Native Barcoding of 1-12 and 13-24 (nuclease free) required for multiplexing and sequencing library preparation, compatible with Promethion sequencer (Oxford Nanopore Technologies).	item12	300	rxn
14	1.13	Ligation Sequencing Kit compatible with Promethion sequencer’s (Oxford Nanopore Technologies) WGS library preparation for sequencing.	item13	300	rxn
15	1.14	Flow Cell Priming Kit for priming flow cells of Promethion (Oxford Nanopore Technologies).	item14	300	rxn
16	1.15	Hot Start High-Fidelity Master mix for cDNA amplification compatible with Promethion (Oxford Nanopore Technologies) sequencer	item15	300	rxn
17	1.16	Random Primer Mix required for cDNA synthesis and compatible for sequencing library preparation to be sequenced on Promethion sequencer (Oxford Nanopore Technologies).	item16	300	rxn
18	1.17	End Repair/dA-Tailing Module is required for cDNA end repairing which is compatible with Promethion (Oxford Nanopore Technologies) library preparation	item17	384	rxn
19	1.18	Ligation Module required for sequencing samples barcoding compatible for library preparation to be sequenced on Promethion (Oxford Nanopore Technologies).	item18	384	rxn
20	1.19	Deoxynucleotide (dNTP) Solution Mix 10mM	item19	3	Vials
21	1.2	Quick Ligation module is required for adapter ligation for sequencing and compatible with Promethion sequencer (Oxford Nanopore Technologies).	item20	300	rxn
22	1.21	COVID-19 primers mix covering the whole genome of SARS CoV2 for whole genome amplification and sequencing.	item21	500	rxn
23	1.22	Rnase inhibitior for molecular biology work, especially used in cDNA preparation	item22	350	rxn
24	1.23	SuperScript™ IV VILO™ Master Mix required for cDNA synthesis compatible with Promethion (Oxford Nanopore Technologies)library preparation and sequencing.	item23	300	rxn
25	1.24	Qubit dsDNA HS assay kit for quantitation of library.	item24	500	rxn
26	1.25	Qubit Assay Tubes for library quantitation.	item25	500	rxn
27	1.26	Qubit RNA ASSAY KIT required for RNA quantitation.	item26	500	rxn
28	1.27	Gel Cassettes for assessing amplicon quality (0.8-1.0 %)	item27	300	rxn
29	1.28	Magnetic beads mix (DNase and RNase free) for cDNA and library purification compatible with Promethion (Oxford Nanopore Technologies) library preparation and sequencing.	item28	300	Nos