Tender For Job Work Contract For Outsourcing Of Farm, Field And Laboratory Operations Services Division Of Genetics And Seed Science & Technology.- 1.01 Wheat 1 2 1.02 Sowing And Covering Of Seeds In 4 M Row 2 1 Per Row 3 1.03 Dibbling / Seeding Manually In Plot ( 2 Rows X 5 M ) 3 1 Per Plot 4 1.04 Emasculation And Pollination 4 1 Per 25 Spikes 5 1.05 Recording Data ( No. Of Tillers, Plant Height, Spike Length, Peduncle Length, Spikelet No. / Spike, Filled Grains / Spike. ) 5 1 Per Plant 6 1.06 Recording Biological Yield Of 1M Row 6 1 Per Meter 7 1.07 Number Of Tillers Per Meter 7 1 Per Meter 8 1.08 Single Spike Threshing 8 1 Per Spike 9 1.09 Single Plant Harvesting & Threshing 9 1 Per Plant 10 1.1 Harvesting And Threshing One Meter Row Length 10 1 Per Row 11 1.11 Harvesting And Threshing Per Plot ( 1.38M X 5M ) 11 1 Per Plot 12 1.12 Harvesting And Threshing Per Plot ( 6M X 1.2M ) 12 1 Per Plot 13 1.13 Harvesting And Threshing Of Plot ( 0.45X 5 M ) 13 1 Per Plot 14 1.14 Wheat Crop Residue Spread 14 1 Per Acre 15 1.15 Biomass Assessment In Wheat Plot 6 X 1.5M 15 1 Per Plot 16 1.16 Mannual Weeding In Wheat 16 1 Per Acre 17 1.17 Preparing Wheat Seed For Off Season Nursery 50- To 100Gm Sample 17 1 Per Sample 18 1.18 Cleaning Of Seed After Harvest ( Per 6X1.5M Plot ) 18 1 Per Plot 19 1.19 Weeding With Kasola - Kharif Season ( Wheat ) Crop ) 19 1 Per Acre 20 1.2 Weeding With Khurpi - Ravi Season ( Wheat ) 20 1 Per Acre 21 1.21 Weeding With Kasola - Kharif Season ( Wheat Crop ) 21 1 Per Acre 22 1.22 Weeding With Khurpi - Ravi Season ( Wheat Crop ) 22 1 Per Acre 23 2 Maize 23 24 2.01 Sowing And Covering Of Seed In 3 M Row 24 1 Per Row 25 2.02 Silking Per Plant 25 1 Per Plant 26 2.03 Tasseling Per Plant 26 1 Per Plant 27 2.04 Separation, Weighing And De-Husking Of Green Cobs Per 5M Row 27 1 Per Row 28 2.05 Harvesting, Collection And Packing Of Cobs Of Single Row Of 3 M 28 1 Per Row 29 2.06 Separation Of Cobs From Plants 29 1 Per Acre 30 2.07 De-Husking Of Cob In 3 M Row 30 1 Per Row 31 2.08 Separation Of Kernel From Cob 31 1 Per Cob 32 2.09 Drying Of Cobs And Seed On Daily Basis 32 1 Per 100 Samples 33 2.1 Harvesting Of Baby Cob Per Picking Per 3 M Row 33 1 Per Row 34 2.11 Data Recording I.E. Plant Stand, Plant Height / Cob Height / No Of Cob, Kernel Row , No Of Kernel, Cob Length , Cob Girth, 100 Kernel Weight ( Per 3M Row ) : 2 Man-Days For 100 Rows Is Needed 34 1 Per Row 35 2.12 Biomass Weight Per Row Of 3 M 35 1 Per Row 36 2.13 De-Tasseling During Seed Production 36 1 Per Acre 37 2.14 Harvesting Male Line / Acre In Seed Production Plots 37 1 Per Acre 38 2.15 Harvesting Female Line In Seed Production Plots 38 1 Per Acre 39 2.16 Maize Crop Residue Spread 39 1 Per Acre 40 2.17 Maize Residue Chopping 40 1 Per Acre 41 2.18 Manual Weeding Maize 41 1 Per Acre 42 2.19 Recording Of Baby Corn Parameters Viz. Length, Width, 5 Cob Weight 42 1 Per 3M Row 43 2.2 Weeding With Kasola - Kharif Season ( Maizecrop ) 43 1 Per Acre 44 2.21 Weeding With Khurpi - Ravi Season ( Maizecrop ) 44 1 Per Acre 45 2.22 Weeding With Khurpi - Khrif Season ( Maizecrop ) 45 1 Per Acre 46 2.23 Weeding With Kasola - Ravi Season ( Maizecrop ) 46 1 Per Acre 47 3 Rice 47 48 3.01 Preparation Of Nursery Beds For Sowing ( Bed Size 4X3 M2 ) 48 1 Per Bed 49 3.02 Preparation Of Bunds In Main Field ( Bund Width Of 75 Cm Each ) 49 1 Per 100 Meters 50 3.03 Making Furrow ( 1 M Each ) For Nursery Sowing Of Single Plant 50 1 Per 100 Furrows 51 3.04 Nursery Sowing And Covering With Soil ( 1 M Each ) 51 1 Per 100 Rows 52 3.05 Nursery Weeding ( Bed Of 4X3 M2 ) 52 1 Per Bed 53 3.06 Nursery Uprooting ( 1 M Each ) 53 1 Per 100 Rows 54 3.07 Shifting Of Nursery To Main Field 54 1 Per 10 Trays 55 3.08 Manual Leveling Of Field Before Transplanting 55 1 Per 100 M2 56 3.09 Field Layout Before Transplanting 56 1 Per 1000 M2 57 3.1 Transplanting Of Trials ( 12 M2plot ) 57 1 Per 1000 M2 58 3.11 Handling Of Rope During Transplanting 58 1 Per 1000 M2 59 3.12 Transplanting Of Bulk Material 59 1 Per Acre 60 3.13 Fixing Of Isolation Sheet In Hybrid Seed Production Plot ( Digging 1 Feet Deep Hole, Fixing Bamboo In It And Stitching Isolation Sheet On It ) 60 1 Per 10 Meter / 10 Bamboo 61 3.14 Transplanting Of Cms Multiplication / Hybrid Seed Production Plot 61 1 Per 100 M2 62 3.15 Transplanting Of Breeding Material ( Sp Progenies ) / Germplasm / Ril’S 62 1 Per Row ( 4.5M Each ) 63 3.16 Cleaning And Maintenance Of Bunds In Main Field 63 1 Per 100 Meters 64 3.17 Rouging In Cms Multiplication / Hybrid Seed Production Plot 64 1 Per 100 M2 65 3.18 Rope Pulling In Cms Multiplication / Hybrids Seed Production Plot 65 1 Per 100 M2 66 3.19 Spraying Of Ga3 Using Knapsack Sprayer 66 1 Per 100 M2 67 3.2 Blb Inoculation 67 1 Per 100 Plants 68 3.21 Recording Of Blb Lesion Length ( 5 Leaves Per Plant ) 68 1 Per 100 Plants 69 3.22 Hand Emasculation And Pollination In Normal Varieties 69 1 Per 10 Panicles 70 3.23 Hand Emasculation And Pollination In Cms Lines 70 1 Per 10 Panicles 71 3.24 Spraying Of Insecticides / Herbicides / Fungicides 71 1 Per Acre 72 3.25 Harvesting And Threshing Of Single Plant 72 1 Per 100 Plants 73 3.26 Collection Of Panicles From Plots / Hills 73 1 Per 100 Panicles 74 3.27 Recording Single Plant Data On Yield And Yield Components ( Tiller Number, Plant Height, Panicle Length ) 74 1 Per 100 Plants 75 3.28 Counting Of Filled Grains / Panicle 75 1 Per 50 Panicles 76 3.29 Cleaning And Drying Of Seeds Of Trial 76 1 Per 100 Kg 77 3.3 Harvesting And Threshing 77 1 Per Plot Of 12 M2 78 3.31 Bulk Harvesting And Threshing 78 1 Per 1000 M2 79 3.32 Removal Of Isolation Sheet And Bamboo 79 1 Per10m X10bambo 80 3.33 Mixing Of B / R Lines Seedlings ( Different Dates ) Before Transplanting 80 1 Per 100 M2 81 3.34 Irrigations In Nursery Bed 4X3m2 81 1 Per Bed 82 3.35 Application Of Micronutrient Solution In Nursery Bed Using Knapsack Sprayer ( Bed Size 4X3m2 ) 82 1 Per Bed 83 3.36 Removing Of Flouting Crop Residues / Debris In Puddled Main Field Before Transplanting 83 1 Per 100 M2 84 3.37 Weeding In ( Rice Crop ) 84 1 Per Acre 85 4 Pearl Millet 85 86 4.01 Opening The Furrow With Plough, Sowing And Covering The Seed Per 3 M Row 86 1 Per 100 Row 87 4.02 Selfing 87 1 Per Spike 88 4.03 Emasculation And Pollination 88 1 Per Spike 89 4.04 Screening Of Male Fertile / Sterile Plants Of 3M Row ( @20 Plants Per 3M Row ) 89 1 Per Row 90 4.05 Data Recording ( Includes Plant Height, Spike Length, Spike Thickness, No. Of Tillers ) 90 1 Per Plant 91 4.06 Harvesting Of Spike Per 3M Row 91 1 Per Row 92 4.07 Harvesting Of Plants From Base And Removal From Field Of 3M Row 92 1 Per Row 93 4.08 Harvesting Bundling And Taking The Spikes Out 93 1 Per 5 Meter Row 94 4.09 Weighing Of Spikes And Fodder Of 3M Row Basis 94 1 Per Row 95 4.1 Threshing Single Ear Head Basis 95 1 Per Single Ear 96 4.11 Threshing Row Basis ( 3M Row ) 96 1 Per Row 97 4.12 Removal Of Spikes And Their Threshing 97 1 Per Acre 98 4.13 General Harvesting Of Spikes 98 1 Per Acre 99 4.14 Weeding With Kasola - Kharif Season Pearl Millet ) 99 1 Per Acre 100 4.15 Weeding With Khurpi - Ravi Season ( Pearl Millet ) 100 1 Per Acre 101 4.16 Weeding With Khurpi - Khrif Season ( Pearl Millet ) 101 1 Per Acre 102 4.17 Weeding With Kasola - Ravi Season ( Pearl Millet ) ) 102 1 Per Acre 103 5 Chickpea, Moongbean, Lentil, Soybean 103 104 5.01 Emasculation And Pollination 104 1 Per 20 Buds 105 5.02 Harvesting & Threshing Single Row Of 2 M Length 105 1 Per Row 106 5.03 Harvesting & Threshing Single Row Of 5M Length 106 1 Per Row 107 5.04 Preparation Of Seed Packet For Sowing 107 1 Per 100 Pkt. 108 5.05 Harvesting And Threshing Single Plant 108 1 Per Plant 109 5.06 Harvesting & Threshing Of Plots ( 5M X1.5M ) 109 1 Per Plot 110 5.07 Harvesting And Threshing Of Plot ( 5M X 2.1 M Plot ) 110 1 Per Plot 111 5.08 Recording Data On Single Plants ( Plant Height, Counting Primary And Secondary Branches, Pods, Seeds Per Pod ) 111 1 Per Plant 112 5.09 Harvesting And Threshing - General 112 1 1Acre 113 5.1 Weeding With Kasola - Kharif Season ( Pearl Chickpea, Moongbean, Lentil, Soybean ) 113 1 Per Acre 114 5.11 Weeding With Khurpi - Ravi Season ( Pearl Chickpea, Moongbean, Lentil, Soybean ) 114 1 Per Acre 115 5.12 Weeding With Khurpi - Khrif Season ( Chickpea, Moongbean, Lentil, Soybean ) 115 1 Per Acre 116 5.13 Weeding With Kasola - Ravi Season ( Chickpea, Moongbean, Lentil, Soybean ) 116 1 Per Acre 117 6 Pigeon Pea 117 118 6.01 Emasculation And Pollination 118 1 Per 50 Buds 119 6.02 Selfing 119 1 Per 50 Buds 120 6.03 Selfing Of Individual Inflorescence By Selfing Bags 120 1 Per 50 Buds 121 6.04 Screening Of Male Fertile / Sterile Plants In 3 M Row 121 1 Per Row 122 6.05 Plot Harvesting And Threshing ( 4.0M X 1.8 M Plot ) 122 1 Per Plot 123 6.06 Plot Harvesting And Threshing ( 4.0M X 5.0 M Plot ) 123 1 Per Plot 124 6.07 Harvesting, Threshing And Cleaning Of 5 M Row 124 1 Per Row 125 6.08 Harvesting, Threshing And Cleaning Of 3 M Row 125 1 Per Row 126 6.09 Harvesting, Threshing And Cleaning Of Single Plants 126 1 Per Plant 127 6.1 Single Plant Field Observations ( Plant Height, Primary & Sec Branches, Main Shoot Length, Pod Length, Seeds Per Pod, 100 Seed Wt, Plant Type-Dt / Idt / Erect / Spreading, Flower Colour And Seed Colour Etc ) 127 1 10 Plants / Entry 128 6.11 Weeding With Kasola - Kharif Season ( Pigeon Pea ) 128 1 Per Acre 129 6.12 Weeding With Khurpi - Ravi Season ( Pigeon Pea ) 129 1 Per Acre 130 6.13 Weeding With Khurpi - Kharif Season ( Pigeon Pea ) 130 1 Per Acre 131 6.14 Weeding With Kasola -Ravi Season ( Pigeon Pea ) 131 1 Per Acre 132 7 Mustard 132 133 7.01 Emasculation And Pollination 133 1 Per 100 Buds 134 7.02 Selfing Single Plant With Three Ring Selfing Bags Supported With Bamboo Sticks 134 1 Per 25 Plants 135 7.03 Selfing Of Brassica Inflorescence With Selfing Bags 135 1 Per 25 Bags 136 7.04 Harvesting And Threshing Of Single Row Of 5M 136 1 Per Row 137 7.05 Single Plant Field Observations ( Plant Height, Primary & Sec Branches, Main Shoot Length, Siliquae On Main Shoot, Seeds / Siliqua, Siliqua Length, Point To First Branch ) 137 1 Per Plant 138 7.06 Erecting Pipes For Selfing ( In 50 Cm Deep Hole For 1.5” Pipe ) + Putting Pipes On In 20” X 20” Square 138 1 Per Pipe 139 7.07 Putting Nets On Pipes ( 10 M X 5 M X 3 M ) 139 1 Per Net 140 7.08 Inoculation For White Rust / Sclerotinia Stem Rot 140 1 Per 25 Plants 141 7.09 Single Plant Harvesting And Threshing 141 1 Per Plant 142 7.1 Plot Harvesting And Threshing ( 5 M X 1.5M ) Plot After Removing Border Row 142 1 Per Plot 143 7.11 Plot Harvesting And Threshing ( 5 M X 2.7M ) Plot After Removing Border Row 143 1 Per Plot 144 7.12 Weeding With Kasola - Kharif Season Mustard ) 144 1 Per Acre 145 7.13 Weeding With Khurpi -Ravi Season ( Mustard ) 145 1 Per Acre 146 7.14 Weeding With Khurpi - Khrif Season ( Mustard ) 146 1 Per Acre 147 7.15 Weeding With Kasola - Ravi Season ( Mustard ) 147 1 Per Acre 148 8 Common Field Work 148 149 8.01 Field Layout 149 1 Per Acre 150 8.02 Plot Sowing By Hand Plough 150 1 Per Acre 151 8.03 Plot Sowing With Tractor Mounted Machine 151 1 Per Acre 152 8.04 Marking Lines With Marker In The Field 152 1 Per Acre 153 8.05 Dusting ( Manually ) Of Crop With Pesticide 153 1 Per Acre 154 8.06 1000 Seed Counting ( Mustard And Pearl Millet ) 154 1 Per 10 Samples 155 8.07 100 Seed Counting In All Crops 155 1 Per 50 Samples 156 8.08 Seed Treatment Per Packet ( 50-200 G ) 156 1 Per 50 Packets 157 8.09 Preparation Of Seed Packets 157 1 Per 50 Packets 158 8.1 Hand Cleaning Of Seed 158 1 Per Kg 159 8.11 Hand Cleaning Of Seed Samples ( 50-200G ) 159 1 Per Sample 160 8.12 Fixing Bamboo / Plastic Sticks For Labeling 160 1 Per 100 Sticks 161 8.13 Tying Of Labels On Plant / Sticks 161 1 Per 100 Labels 162 8.14 Selfing Of Single Plants With Net Bag Supported With Bamboo 162 1 Per Plant 163 8.15 Selfing Of Plots- Size 3 M X 3.6 M With Net Bag Supported With Bamboo 163 1 Per Plot 164 8.16 Hand Weeding In Field 164 1 Per Acre 165 8.17 Weeding Of Net Houses 400 Sqm Area 165 1 Per Net House 166 8.18 Cleaning Around The Net Houses 166 1 Per Net House 167 8.19 Deep Digging Net-House 6 Inch Deep 167 1 Per M2 Square 168 8.2 Path And Bund Cleaning 168 1 Per Acre 169 8.21 Bird Scaring Per 5 Acre Plot ( Two Shift ) 169 1 Per 5 Acre 170 8.22 Bird Scaring Per 4 Acre Plot ( Two Shift ) 170 1 Per 4 Acre 171 8.23 Bird Scaring Per 3 Acre Plot ( Two Shift ) 171 1 Per 3 Acre 172 8.24 Bird Scaring Per 2.5 Acres Plot ( Two Shift ) 172 1 Per 2.5 Acres 173 8.25 Bird Scaring Per 2 Acre Plot ( Two Shift ) 173 1 Per 2 Acre 174 8.26 Bird Scaring Per One Acre Plot ( Two Shift ) 174 1 Per Acre 175 8.27 Bird Scaring In Pearlmillet And Maize At Maturity ( Two Shift ) 175 1 Per Acre 176 8.28 Bird Scaring In Pearlmillet And Maize At Maturity ( Two Shift ) 176 1 Per 2.5 Acres 177 8.29 Bird Scaring In Pearlmillet And Maize At Maturity ( Two Shift ) 177 1 Per 5.0 Acre 178 8.3 Irrigation 178 1 Per Acre 179 8.31 Harvesting And Threshing With Machines 179 1 Per Acre 180 8.32 Bulk Threshing Of Crop - General 180 1 Per Acre 181 8.33 Bulk Harvesting Of Crop- General 181 1 Per Acre 182 8.34 Harvesting And Threshing Of Crop -General 182 1 Per Acre 183 8.35 Cleaning Of Harvested Seed In Field / Lab 183 1 Per 50 Kg 184 8.36 Gap Filling By Transplanting 184 1 Per Acre 185 8.37 Rouging 185 1 Per Acre 186 8.38 Thinning ( Mustard, Rice And Pearl Millet, Maize, Wheat ) 186 1 Per Acre 187 8.39 Thinning ( Soybean, Chickpea, Mungbean, Pigeon Pea, And Other Crops ) 187 1 Per Acre 188 8.4 Spreading Of Fym In The Field 188 1 Per Acre 189 8.41 Application Of Fertilizers ( All Crops ) 189 1 Per Acre 190 8.42 Hill Application Of Fertilizers / Granules 190 1 Per Acre 191 8.43 Preparation Of Sticks / Pegs ( 90 Cm ) From Raw Bamboo 191 1 Per 100 Sticks / Pegs 192 8.44 Spray Of Pesticides With Tractor Sprayer In Field 192 1 Per Acre 193 8.45 Spray Of Pesticides With Knap Sack / Foot Pump Sprayer In Field 193 1 Per Acre 194 8.46 Removal Of Crop Residue Out From The Field -General 194 1 Per Acre 195 8.47 Threshing Of Single Spike In Wheat / Panicle In Rice 195 1 Per Panicle / Spike 196 8.48 Roughing In Nucleus / Breeder Seed Plots 196 1 Per Acre 197 8.49 Transplanting Of Rice For Nucleus Seed 197 1 Per Acre 198 8.5 Transplanting Of Vegetables 198 1 Per Acre 199 8.51 Preparing Beds ( Flat / Ridge ) For Vegetable Sowing / Transplanting 199 1 Per Acre 200 8.52 Field Emergence, Speed Of Emergence And Plant Stand Count Of 5 Meter Row Length 200 1 Per Row 201 8.53 Inoculation Of Plants 201 1 Per 25 Plants 202 8.54 Bulk / Single Plant Observation Of Dus Characters ( Ten Traits ) 202 1 Per 25 Plants 203 9 Common Lab, Field Work Across Crops , Seed Testing Activities And Pot Culture Etc 203 204 9.01 Sample Preparation Of Leaf / Seed For Quality Analysis 204 1 Per 25 Samples 205 9.02 Tagging Of Single Plants And Collection Of Leaf Samples In Liquid Nitrogen 205 1 Per 25 Samples 206 9.03 Plant-Wise Harvesting Of Genotyped Pollinator Plants 206 1 Per 25 Plants 207 9.04 Grinding Of Samples For Dna Isolation ( 5 G Leaf ) 207 1 Per 25 Samples 208 9.05 Preparation Of Glassware And Plastic Ware For Rna Work Through Naoh Treatment Depac Water Treatment Sterilized Through Autoclaving 208 1 Per 25 Pieces 209 9.06 Sample Preparation For Enzyme / Hormone Analysis 209 1 Per 25 Samples 210 9.07 Washing And Autoclaving Of Mortar-Pestle And Glass And Plastic Wares 210 1 Per 100 Pieces 211 9.08 Air Drying, Grinding And Sieving Of Soil Samples In The Laboratory 211 1 Per 25 Samples 212 9.09 Bread Making 100 G Sample In Wheat 212 1 Per 25 Samples 213 9.1 Biscuit Making 500 G Sample In Wheat 213 1 Per 25 Samples 214 9.11 Chapatti Making 300 G Sample In Wheat 214 1 Per 25 Samples 215 9.12 Help In Sedimentation Analysis In Wheat 215 1 Per 25 Samples 216 9.13 Brix Value Estimation / Cob For Sweet Corn / Green Cob ( Maize ) 216 1 Per 50 Cobs 217 9.14 Estimation Of Popping Value ( 200 Kernels Per Sample ) In Maize 217 1 Per 50 Samples 218 9.15 Estimation Of Kernel Opaqueness Through Light Box ( 200 Seeds / Sample ) In Maize 218 1 Per 50 Samples 219 9.16 Grinding Of Maize Seed ( 50-100 Seeds ) Samples For Biochemical Estimation 219 1 Per 50 Samples 220 9.17 Analysis Of Pollen Fertility In All Crops 220 1 Per 25 Samples 221 9.18 Hulling By Palm Husker Of 25 Grains / Sample In Rice 221 1 Per 25 Samples 222 9.19 Hand Milling Of 25 Grains / Sample In Rice 222 1 Per 25 Samples 223 9.2 Grinding Of Rice Grains Of 4G / Sample 223 1 Per 25 Samples 224 9.21 Hulling And Milling Of Trial Samples Of 200G Paddy In Machines 224 1 Per 25 Samples 225 9.22 Preparation Of Samples, Cooking And Display Of Cooked Rice From Single Plant Selections In Petridishes ( 10 Grains Per Entry ) 225 1 Per 25 Samples 226 9.23 Preparation Of Samples And Data Recording Of Rice Grain Dimensions ( Kernel Length, Width ) Before And After Cooking 226 1 Per 25 Samples 227 9.24 Separation Of Head Rice From Milled Rice Samples Obtained From 200G Paddy Samples 227 1 Per 25 Samples 228 9.25 Filling Of Pots With Soil For Sowing 228 1 Per 25 Per Pot 229 9.26 Filling Of Pots And Shifting Plants In Pots 229 1 Per 25 Pots 230 9.27 Filling Of Pots ( 4 ) 230 1 Per 25 Pots 231 9.28 Sowing In Pots ( 4 ) 231 1 Per 25 Pots 232 9.29 Filling Of Trays ( 10 X 5 X 3 ) 232 1 Per 25 Trays 233 9.3 Sowing And Planting In Pots 12 Inch X 14 Inch Pots 233 1 Per 25 Pots 234 9.31 Empting Of Pots All Sizes 234 1 Per 25 Pots 235 9.32 Sowing In Tray 9 X 4 Inch Tray 235 1 Per 25 Pots 236 9.33 Preparation And Sowing Of Seeds In Plastic Cups 236 1 Per 25 Samples 237 9.34 Pot Filling And Mixing Of Soil With Fym 12 Inch X 14 Inch Pots 237 1 Per 25 Trays 238 9.35 Weeding And Inter-Culture In Pots 238 1 Per 25 Pots 239 9.36 Irrigation In Pots 239 1 Per 25 Pots 240 9.37 Laboratory Analysis Of Soil And Plant Samples 240 1 Per 25 Pots 241 9.38 Collection Of Soil Samples From The Field, Mixing And Transporting 241 1 Per 25 Samples 242 9.39 Collection, Processing And Grinding Of Plant Samples 242 1 Per 25 Samples 243 9.4 Sample Preparation For Physical Purity 243 1 Per 25 Samples 244 9.41 Seed Purity Analysis 244 1 Per 25 Samples 245 9.42 Media ( Substratum ) Preparation Towel Paper ( Bp ) , Top Of Paper ( Tp ) , Sand And / Or Agar Medium 245 1 Per 25 Samples 246 9.43 Seedling Evaluation 246 1 Per 50 Samples 247 9.44 Planting Of Samples In Different Substratum For Seed Germination 247 1 Per 50 Samples 248 9.45 Preparation Of Seed Samples For Electrical Conductance 248 1 Per 50 Samples 249 9.46 Reading Of Electrical Conductance From Soaked Seed 249 1 Per 100 Samples 250 9.47 Preparation And Conditioning Of Seed Samples For Accelerated Ageing Test ( Aat ) Under Controlled Environment ( 400C, 100% Rh ) 250 1 Per 100 Samples 251 9.48 Preparation Of Seed Samples For Seedling Growth 251 1 Per 100 Samples 252 9.49 Seedling Measurement 252 1 Per 100 Samples 253 9.5 Preparation And Conditioning Of Seed Samples For Tetrazolium Test ( Tz ) 253 1 Per 25 Samples 254 9.51 Evaluation Of Embryo Staining 254 1 Per 25 Samples 255 9.52 Filling Of Tip Boxes And Sterilization 255 1 Per 25 Box 256 9.53 Preparation Of Samples For Germination Test 256 1 Per 25 Samples 257 9.54 First Count During Germination Test 257 1 Per 25 Samples 258 9.55 Final Count During Germination Test 258 1 Per 25 Samples 259 9.56 Root And Shoot Length Observations On 10 Seedlings From Each Sample 259 1 Per 25 Samples 260 9.57 Dry Weight Observation On 10 Seedlings From Each Sample 260 1 Per 25 Samples 261 9.58 Preparation Of Solutions / Chemicals For Seed Treatment 261 1 Per 25 Samples 262 9.59 Treating The Seed With Various Chemicals / Coating Priming Etc. 262 1 Per 25 Samples 263 9.6 Plating Of Fungi 263 1 Per 25 Plates 264 9.61 Counting 100, 50 And 25 Per Seeds Sample 264 1 Per 25 Samples 265 9.62 Cleaning Of Seed And Leaf Samples 265 1 Per 100 Samples 266 9.63 Maintenance Of Temp. And Humidifier In The Walk-In Germination Chambers 266 1 Per Unit / Month 267 9.64 Storage And Upkeep Of Guard Samples 267 1 Per 100 Samples 268 9.65 Moisture Content Estimation 268 1 Per 100 Samples 269 9.66 Maintenance And Upkeep Of Glass House , Ne House And Surrounding Area ( 100 M2 ) 269 1 Per Month 270 9.67 Temperature And Humidity Controlled Seed Store Cleaning Of Chillers Unit, Water Storage Tank Clean & Cleaning Of Filters Fitted In Dehumidifiers And Cooling Coil, Cleaning Of Racks Of Seed Storage Room 270 1 Per Month 271 9.68 Cleaning / Dusting Of Machines–Seed Dryer, Seed Air And Screen Cleaner, Gravity Separator, Seed Coating, In The Process. Lab. 271 1 Per Month 272 9.69 Maintenance And Up Keeping Of Net House And Surrounding Area 100Sq Meter 272 1 Per Month 273 9.7 Preparation Of Fly Food And Subculture 273 1 Per 50 Bottles