Delhi Jal Board-DJB has published Procurement Of Realtime Pcr As Per Specifications And Bq Make- Thermofisher/ Biorad/Himedia/ Analytika Jena/ Agilent. Submission Date for this Tender is 16-03-2020. PCR and RT PCR Products Tenders in CHIEF WATER ANALYST (WnS)-III Delhi. Bidders can get complete Tender details and download the document.
| Name of Work:N.I.T No: 20 (2019-20) Procurement of Realtime PCR as per specifications and BQ Make- THERMOFISHER/BIORAD/HIMEDIA/ANALYTIKA JENA/AGILENT | ||||
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| Item Description | Item Code / Make | Quantity | Units | |
| 1 | Details and Specifications of RTPCR :-Two Channel and Upgradable to Five Color Realtime PCR System - Excitation Source 8 dye specific LEDs per optical module ..As Per BOQ\nDetection Sources 8 silicon photo-detectors per optical module\nDyes SYBR/FAM/TB daye 462.5 – 516.0nm \nDetectable Ct for 0.001ng/DNA per reaction (R2 > 0.980) \nHEX 535.0 – 555.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980) \nROX 585.0 – 610.0nm Detectable Ct for 0.001ng/DNA per reaction (R2> 0.980) \nCY3 542.0 – 568.5nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980) \nCY5 635.0 – 665.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980) \nAtto 425 – 435 – 475.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980) \n6 slots, swappable cartridges \nCustom cartridges, available upon request\nDye Selection Excitation and emission\nReaction Volume 96 well Block with 10µl to 30µl reaction volume capacity \nChemistries Supported SYBR, LC green , Takarabio Green dye , Taqman Probe, HRM\nThermal System Six peltiers made from two ceramic plates with semi-conductor elements, 96 well\nThermal System Temperature Range 25.0 – 99.9°C Heating: 6°C/sec Cooling: 2.5°C/sec Accuracy: ± 0.2°C\nDynamic Range 9 orders of magnitude\nComparative Quantitation 2x (Range 1.3x to 3x), 10x (Range 6.5x to 15x)\nMultiplex 2 Channel system up -gradable to Five Channel /color\nSensitivity (FAM) Discriminates between 2 fold populations ranging from 100k to 12 copies with 95% confidence\nCq uniformity Cq St Dev <0.20 at fast cycling (5sec 95°C/10sec 60°C)\nUniformity ± 0.25°C\nTm Uniformity with HRM ± 0.1°C St. Dev. Less than or equal to 0.03°C\nData acquisition time 3 sec for data acquisition in all six channels\nElectrical Power (input) 100 – 240VAC, 50/60Hz, 1100A\nOperating Environment 18 – 35°C (65 – 95°F), 85% non-condensing humidity 7500 feet, max altitude\nWeight 50 lbs. (23 kg)\nDimensions 19.7” W x 18.1“ D x 16.5” H (50cm x 46cm x 42cm)\nSample Containers 0.2ml tubes, 0.1 ml tubes 96 well plates, strip tubes\nWarranty • 1 year warranty is standard with the instrument \nOnboard Analytics • Thermal, physical, interactive (sensors) tests \n• Extended: 120 performance points tested in 30 minutes \n• Start-up: 20 performance points tested in ~1 minute \n• Optional bypass of both features\nInstallation & Demonstration \n\n\n\n\nTraining • Installation and familiarization with Following kits \nhepatitis A , Enterovirus , Vibrio Cholera &\nSalmonella . \n• The above application should be demonstrated for successful installation . The Installation will only be completed if the above demonstration is successful . \n\nComplete training should be provided to run the system and do the test as per BIS norms for PCR based water testing . \n \nRun Modes • Stand alone \n• PC connected \n• LAN connected: more than 20 instruments can be connected and monitored remotely \n• Two USB ports for extending to external devices.\nSoftware : Included\nWarranty : 02 years\nKits include : 2X 100 test for each -:Hepatitis A, Enterovirus, Vibrio cholera and Salmonella\nExtraction Kits : 2X 100 test for each-: DNA & RNA\n\nMake:- THERMOFISHER/BIORAD/HIMEDIA/ANALYTIKA JENA/AGILENT\n | item1 | 1 | Nos |
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