tender for supplying and commissioning of following items for the project of nmhs project nmhs2024-25/sc-xii/mg96-201 at department of zoology, ballygunge science college campus, university of calcutta. , whole transcriptome sequencing & its analysis sample type- fish tissue • platform-illumina novaseq6000 • sample no-15 • library type-pe150 • depth/sample-minimum 15 gb/sample • data normalisation & bioinformatics analysis-yes • turn around time-within 3 months or less (if the sample has passed the quality control) • data delivery-server-based or external storage device • sample submission requirement , wgs (denovo sequencing) platform-illumina novaseq6000 • sample no-10 • library type-pe150 sequence output: min 100x sample type: fish dna sample with extraction, quality control, library preparation , sanger sequencing with column purification sample type: fish dna data analysis & sequencing within 5 days total sample: 750 , fish estradiol (e2) elisa kit , fish 11-keto testosterone (11-kt) elisa kit , fish 17alpha, 20beta dihydroxy progesterone (17alpha, 20betaoh-prog) elisa kit , qiaamp dna mini kit (50) , le agarose , gotaq green master mix