in vitro mutagenesis of stevia for enhanced production of steviol glycosides- equipment purchase for scientific research , particulars , fully-automated microtome : it should have precise stepper motor and drives which ensure accurate specimen advance and maintenance free cross roller guide ways which provides greater stability during sectioning. • it should have a powerful sectioning motor and sectioning speed can also be adjusted. • lcd screen displays section and trimming thickness, number of sections cut travel. trimming thickness, sectioning thickness, fast forward and fast backward movement can be controlled through feather touch keypad. the hand wheel should have lockable in any position, huge volume waste tray. it should have an integrated safety alarm system and emergency stop button. • it should have section thickness range 0.5-200?m. ( to set up to 200?m, 200?m, can change as per requirement. ) • it should have section thickness setting 0.5-5?m, in 0.5?m increment 5-20?m, in 1?m increment 20-60?m in 5?m increment 60-100?m in 10?m increment 100-200?m in 20?m increment • trimming thickness setting1-800?m. ( you can set any as per your requirement ) • setting values- 1-10?m in 1?m increment 20-50?m in 5?m increments 50-100?m in 10?m increment 100-600?m in 50?m increment 600-800?m in 100?m increment • it should have sectioning modes: 4 continuous, step, programmed, manual. • it should have horizontal feed28mm. • it should have vertical specimen stroke60mm • sectioning speed adjustable, 3 different speeds. slow, medium and high • specimen retraction 5-100?m in 5?m increments, can be turned off • max specimen size 50x50 mm.• specimen orientation horizontal 8°, vertical 8°, z 360°• nominal supply voltages 110 / 220 v ac ± 10%. • nominal frequency 50 / 60 hz • dimensions basic instrument 590 x 345 x 290 mm ( w x d x h ) • ce certified product from european notified body and it should be usfda registered.• iso 9001 and who gmp certified manufacturer on-site warranty: minimum 2 years , cooling incubator-shaker : capacity: 113 lit. 4 cu. ft. cap., shaker cap.: 250 ml x 9 flasks & 100 ml x 9 flasks, inner & outer being made of ss sheet, temp. range 5°c - 60°c, controlled by digital indicator cum controller, accuracy of + / - 0.5c. 1 no. shaker constructed at the bottom of the working chamber with speed control, shaking speed 50 - 250 rpm. provided with a motorized fan blower. 230 v. ac. the manufacturer should have a service set-up at kolkata & those document needs to be enclosed along with your bid. manufacturer should submit their factory license certificate along with their bid. ce & iso ( 9001 : 2015 & 13485 : 2016 ) approved on-site warranty: minimum 2 years