supply of reagents and chemicals for making a rate contract for 2 years - 1 10140 indicator papers-full range ( ph-1.0 to 14.0 ) nfull range ( ph-1.0 to 14.0 ) n 2 1kb dna ladder n1 vial = 50 μg n 3 1m tris ph 7.4 ( molecular grade ) 4 absolute alcohol nmolecular grade n 5 acetic acid glacial, ar grade 6 acetone ar sq nar grade n 7 acrylamide ( molecular grade ) nmolecular grade n 8 agarose ( molecular grade ) 9 bisacrylamide ( molecular grade ) 10 bleaching powder isi marked 11 boric acid ( molecular grade ) 12 cedar wood oil ( immersion oil ) sq 13 conc. hcl. 14 crystal violet powder nar grade n 15 disinfectant surface cleanser ( benzalkonium chloride 11.5% w / w, non ionic surfectants ) 16 dntp mix 10 mm each 17 dpx 18 edta ( molecular grade ) 19 edta dipotassium salt dihydrate gr ngr grade n 20 eosin y 21 ethanol ( 100% ) : molecular grade 22 ether ( anaesthetic ) 23 ethidium bromide nfor agarose gel electrophoresis n 24 formalin 25 giemsa 26 glycerol nar grade n 27 gold chloride 28 haematoxylin crystals 29 hydrogen peroxide 30% sq nsq grade, purified n 30 india ink nliquid non-water proof n 31 isopropyl alcohol molecular grade 32 leica disposable blades low profile ( 819 ) : 80 mm x 8 mm 33 leishman stain powder 34 malachite green nsq grade n 35 methanol sq 36 methanol nabsolute acetone free n 37 methylene blue powder nar grade n 38 mgcl2 ( molecular grade ) 39 new methylene blue ( retic stain ) 40 nnnn tetra methyl p phenylene diamine dihydrochloride nar grade n 41 nuclease free water 42 nusieve agrose ( molecular grade ) 43 paraffin oil 44 paraffin wax ( 58-60 0c ) 45 potassium alum ( aluminium potassium sulfate ) 46 potassium dicromate nar grade n 47 proteinase k nchromatographicaly purified 30 amanson u / mgm n 48 sodium chloride ar 49 sodium dihydrogen phosphate ar 50 sq liquid ammonia solution ap.gr. 0.91 ( about 25% nh3 ) 51 sulphuric acid ( 95-98% ) nsq grade n 52 taq dna polymerase ( recombinent ) 53 temed ( molecular grade ) 54 tris base nmolecular grade n 55 turks fluid 56 urea ( bacteriological ) nar grade n 57 xylene nsq grade n 58 n / 10 hcl 59 22-25 mer oligonucleotide primer, standard desalted 50 nmol 60 6x gel loading dye 61 gene ruler 50 bp plus dna ladder nmolecular grade n 62 pcr master mix ( 2x ) , mmx 2x for 200 reactions 63 co-enzyme nadh 64 catalase 65 wright stain 66 poly-l-lysine solution 67 ehrlich reagent 68 normal saline 69 buffer capsule ph 4.0 70 buffer capsule ph 7.0 71 edta ( protease, nuclease free ) 0.5m 72 ultra pure distilled water nmolecular grade, dnas & rnas free n 73 inulin nfor differentiation of s. pheummoniae n 74 urea agar base ( christensen ) nfor detction of ureas production n 75 d- glucose nar grade n 76 sucrose nar grade n 77 lactose nar grade n 78 d - mannitol nar grade n 79 ammonium per sulphate 80 ammonium chloride 81 sodium bi-carbonate 82 dimethyl sulphoxide ( dmse ) nmolecular grade n 83 fouchets reagent