tender for supply of hplc system for accurate estimate of cinchona alkaloids and active chemical profiles of map hplc system for accurate estimate of cinchona alkaloids and active chemical profiles of map (2nd call) , waters advanced fully integrated quaternary gradient hplc system with accessories (detail specification provided in nit no. 16/dcomp/ten/2024 dated 15/11/2024) , (i) waters advanced fully integrated quaternary gradient hplc system with accessories (-solvent management system (quaternary pump) - sample management (auto sampler) - sample temperature control - column temperature control (column heater) (ii) alliance d series bottle tray (iii) detector 2489 uv/visible detector (iv) software with accessories waters advanced empower software with system suitability & 5 nos. named user license (v) columns c18 5?m 4.6x250mm column c8 5?m 4.6x250mm column (vi) instrument along with detectors and software qualification service & certification hplc system qualification (iq, oq) with detector (vii) essential accessories suitable computer with printer suitable 2kva on-line ups with 30 min backup , other items (detail specification provided in nit no. 16/dcomp/ten/2024 dated 15/11/2024 , 2414 refractometer detector detection of components without uv chromophores separated by isocratic analysis. auto-zero, auto-purge, and led-displayed diagnostic programming , 2475 fluorescence detector multiple detection modes - 2d, 3d, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence. integral erbium calibration reference – ensures wavelength accuracy. , millipore water purification system smartpak dq3, millipak express 40 final filter, 0.22 um, smt installation, iron removal filter unit, prefiltration system for dq3 , air conditioner (2 tonnes) air conditioning system that provides the necessary environmental conditions for optimal performance and reliability of hplc laboratory. , micropipette 20-200 ?l , micropipette 10-100 ?l , micropipette 0.5 ml-5ml , magnetic stirrer electronically controlled, variable speed and 220 v, 50 hz ac, single phase , dehumidifier touch control, room coverage area, sq. ft. 201-250, humidity range, % rh 40-80 and working temperature 5 degree celsius , hplc vials standard blue 2ml hplc vials, size: 12mm diameter,32mm height, cylindrical , ultrasonic probe sonicator heavy duty probe support system with dual columns, titanium probe, sound enclosure box, air cooling system for the ultrasonic transducer , analytical balance repeatability (standard deviation), mg, linearity, up to +/-1 mg and weighing capacity: 252 gram , vacuum filter inlet funnel outlet flask (receiver), 0.22 ?m pore size, funnel capacity 500 ml and filtration area 40 cm2 , ph meter resolution (ph) 1/2 hp, power (volt) 220 to 240 v and power source electronic , mobile phase bottle 1000 ml , mobile phase bottle 2 ltr , syringe filter 0.45 micrometre membrane diameter25 millimeter, connection typeluer-lok/luer slip, holdup volume<100 microliter , disposable syringe 3 ml , disposable syringe 5 ml , water bath double walled with thermostat