supplying, installation and commissioning of consumable -supplying, installation and commissioning of the noted items for the dbt grant no. bt pr24618 ner 95 779 2017 under dr. dipankar chakraborti at department of genetics, takar nath palit siksha prangan, ballygunge campus, university of calcutta. , service required: whole genome sequencing of insect samples family of the insect: miridae data to be generated: minimum 100 gb clean reads sequencing chemistry: 150 pe sequencer: illumina novaseq 6000 analysis required: • denovo assembly with velvet / ray / soapdenovo / gsassembler • assembled scaffolds • summarized genome assembly statistics: total number of scaffolds total number of contigs total genome length including gaps total genome length without gaps average contigs size contigs n50 / scaffold n50 maximum and minimum scaffold size percentage of a, t, g, c and n in assembly • final assembled genome sequence ( contigs / scaffolds ) in fasta file format • functional annotation of gene: annotated genes with ec number distribution of genes based on go terms when mapped to go database go functional classification of genes in biological processes, cellular components and molecular functions • genome completeness ( busco ) • gene predictions: predicted genes regions in the assembled scaffolds / contigs predicted cds region in the genes peptide sequence of the predicted genes • whole genome comparison between two samples ( snps and indels with functional effect prediction ) • ssr discovery • circos plot for comparative analysis ( against closest reference genome sequence available ) . • comprehensive compiled report and data ( specification as per nit documents )