supply of laboratory reagents and other consumables for research work at department of genetics, ballygunge science college campus under the university of calcutta. , fugene hd transfection reagent, make-promega/ equivalent, cell transfection reagent, can be used with difficult-to-transfect cell lines, primary cells, applicable for protein and virus production. (specification as per nit) , fetal bovine serum, make-gibcco/ equivalent, brazilian origin, sterile-filtered, tested for mammalian cell culture.(specification as per nit) , gotaq green master mix , make: promega/ equivalent, 2x, green master mix for direct-to-gel analysis after amplification, should contain taq dna polymerase, dntps, mgcl2 and reaction buffer, available with nuclease-free water.(specification as per nit) , iscript cdna kit, make- biorad/ equivalent, first-strand cdna synthesis kit, should contain 5x reverse-transcription reaction mix, reverse transcriptase, nuclease-free water. (specification as per nit) , itaq™ universal sybr® green supermix , make: biorad/ equivalent, should contain 2x qpcr mix, containing dntps, itaq dna polymerase, mgcl2, sybr green i, enhancers, stabilizers, and passive reference dyes, ready-to-use. (specification as per nit) , pureyield plasmid miniprep kit, make: promega/ equivalent, plasmid miniprep system fortransfection-ready plasmid from cultures , , should contain cell lysis buffer, neutralization solution, endotoxin removal wash, column wash solution, elution buffer, columns & collection tube. rapid, 10-minute protocol. can be used with centrifugation or vacuum-based protocols. (specification as per nit) , dna isolation kit from sputum, make: qiagen/ equivalent, dna isolation kit from tissues, swabs, csf, blood, body fluids or washed cells from urine. spin column-based procedure, suitable for genomic dna, mitochondrial dna, bacterial dna, parasite dna, viral dna. (specification as per nit) , fluorotrans™ pvdf transfer membrane roll, make-pall/equivalent,detection system chemiluminescence,totalprotein filter media fluorotrans pvdf pore size (removal rating) 0.2 ?m sheet size length (metric cm) 10 cm sheet size width (metric cm) 15 cm typical thickness (metric ?m) 127 ?m pore size - removal rating 0.2 ?m (specification as per nit) , primers 20-25 base with hplc purification , make: idt/ equivalent, high performance liquid chromatography (hplc), a form of column chromatography, can be performed in one of two ways. reverse-phase (rp) hplc separates full-length oligo product from truncated products based on relative hydrophobicity. ion-exchange (ie) hplc separates full-length oligonucleotides from truncated species based on relative charge difference. idt uses hplc to purify unmodified oligos as well as oligos with complex modifications such as linkers, spacers, modified bases, and hydrophobic modifications. the type of hplc selected is dependent on oligo sequence composition and characteristics. (specification as per nit) , anti-beta actin antibody, make: abcam/ equivalent, anti-beta actin antibody, clone id: [mabcam 8226]; mouse monoclonal ab, suitable for: icc/if, ihc-p, wb, reacts with: mouse, rat, human; isotype: igg1. (specification as per nit) , pcr tube 0.2ml flat cap, make: biorad/ equivalent, 0.2 ml pcr tube, flat caps, high profile, clear, full-height wells; polypropylene made with attached cap, free of dnase, rnase, and human dna. (specification as per nit)