purchasing influenza rt- pcr kit , viral rna extraction kit of vrdl microbiology department for r g kar medical college , influenza rt-pcr kit specification: 1. the kit should be ce-ivd approved. 2. the kit should be multiplex one-step taqman pcr based. 3. the kit should work on rna isolated from sputum , balf, oropharyngeal and nasal swabs in vtm 4. the kit should detect flu-a, flu-b, flu-c, h1n1 , h3n2 and h5n1 in a 2-tube multiplex pcr reaction 5. the kit run time should not exceed 1 hr 20 mins 6. the kit should be compatible with appliedbiosystems@7500/7500 fast real-time pcr system , quanstudio 5, bio-rad cfx96 , qiagen rotor gane q, roche lighcycler@480 , analytik jena qtower-g touch series real time pcr platforms. 7. no cross-reactivity with related viruses like rsv, covid-19, metapneumovirus and parainfluenza virus 1-4 should be noted. , viral rna extraction kit specification: 1. the kit should be ce-ivd approved. 2. the kit should be used for the isolation of rna viruses like covid-19, rsv and influenza viruses 3. the kit should use magnetic bead based automated rna extraction in kingfisher flex-96 machine with documented evidence for the same. 4. proteinase – k and other lytic reagents should be included in the lysis buffer and not to be added separately . 5. the entire process should take less than 30 mins per isolation for a batch of 5 samples. 6. sample volume should be 200ul with elution volume 50ul