bids are invited for probe qpcr premix 2x with hot start pcr enzyme with an antitaq antibody suitable for taqman probe based real time qpcr - 5 ml or equivalent , hot start 2x pcr master mix with loading dye added for high-yield conventional pcr -800 reactions of 50 microlitres each or equivalent , spin column based gel extraction kit suitable for purification of dna from excised agarose gel - 250 columns per kit , 2x master mix or kit for taqman probe based real-time rt-qpcr - 5 ml or equivalent , recombinant dnase i - should be rnase-free - 1000 units , proteinase k solution or lyophilized - 5 ml of 20 mg per ml or equivalent , nuclease free ultrapure water - 500 ml , one step rt-pcr kit - 100 reactions of 50 microlit each or equivalent total quantity : 13