procurement of mammalian gene expressing construct procurement of mammalian gene expressing construct , mammalian gene expressing construct -subcloning and codon optimization of genes for mammalian and bacterial expression, subcloning vector pcdna 3.1;pqe80lall the sequences should be codon-optimized for higher-level expression ( soluble form ) in e. coli using gene optimizer technology software and it should be part of the synthetic construct optimization. gene number 2 to 9 should be cloned in the pqe80l using 5’ bamh1 / 3’kpni. gene number 1 should be cloned in the pqe80l and petsumo or pet151 using 5’ ecor1 / 3’kpni. , gene 1 gaattc attaaa gag gag aaa ttaact atg aga