supply of chemicals and reagents , 1-hexane sulphonic acid ar , 1-naphthol ar , 1-propanola r , 2, 4 dinitro -1- fluoro benzene ar , 4-dimethylamino benzaldehyde reagent ar , absolute ethanol ar merck , acetic acid glacial ar , acetic anhydride ar , acetone ar , acetonitrile hplc , aflatoxin mix 4 solution 34036-1ml-r sigma-aldrich ( 2?g / ml b1& g1 and 0.5?g / ml b2 & g2 ) , ammonia solution ar , ammonium acetate ar , ammonium chloride ar , ammonium ferric sulphate dodecahydrate ar , antibiotic assay media d mm004 - 100ghimedia , arsenic trioxide ar , ascorbic acid ar , autoclavable l- spreade himedia pw 1085 , barium chloride ar , benzene ar , benzethonium chloride ( hyamine ) ar , bismuth sulphite agar mo27-100g himedia , boric acid ar , brig 35 ( polyoxyethylene ( 23 ) lauryl ether , bromine solution ar , bromothymolgreen solution ar , buffer standard ph 10.00+ 0.01@250c certified traceable to nist , buffer standard ph 4.00+ 0.01@250c certified traceable to nist , buffer standard ph 7.00+ 0.01@250c certified traceable to nist , calcium chloride dihydrate ar , calcium nitrate ar , calcon carboxylic acid indicator ar , carbon disulphide ar , carbon tetrachloride ar , cetrimide agar mh024- 100ghimedia` , charcoal activated ar , chloroform ar , chloroform uv , citric acid anhydrous ar , citric acid monohydrate ar , cleansol , cobalt chloride hexahydrate ar , conductivity solution 10?s / cm , copper sulphate ar , cyclohexane hplc / uv , dichloro methane ar , dichloro methane hplc , diethyl ether ar , diethylamine emparta- merck , dimethyl sulphoxide ar , diphenylamine barium sulfonate ar , dipotassium hydrogen phosphate anhydrousar , disodium hydrogen orthophosphate anhydrous ar , disodium oxalatecas no:62-76-0 sku-1.02407.0060 sigma aldrich ( merck ) , dried methanol ( water 0.02% max ) ar merck , durhoms tubes himedia gw163 ( 1 x 100 nos ) , edta disodium salt ar , ethyl acetatear , fehling solution a , fehling solution b , ferric chloride anhydrous ar , ferrous ammonium sulphate ar , ferrous sulphide pellets ar fused sticks , fluid thioglycollate meidum mu009-500ghimedia , folin ciocalteu reagent ar , formaldehyde ar , formic acid anhydrous ar , formic acid emparta acsmerck , geobacillus stearothermophilus spores ampoules la 926 , germisol , germitol , glycerin ar , gram stain kit k001 - 1 kit himedia , heptafluorobutyric acid ar , hexamine ar , hydrochloricacid ar , hydrogenperoxide solutionar 30% v / v , hydroquinone ar , hydroxy naphthol indicator ar , isopropyl alcohol ar , imidazole mercury reagenta r , immerson oil for microscopy ( low viscosity ) grm 225-30g himedia , indigo sulfonic acid , iodine trichloride ar , isopropyl alcohol uv , karl-fischer reagent ar , keisulghar ar , lanthanum ( iii ) nitrate hexahydrate ar , l-ascorbic acid ar , l-ascorbic acid sodium ar , lead acetatetrihydrate ar , lead acetate paper , lead nitrate ar , litmus paper blue , litmus paper red , lizol , magnesium sulphate ar , mannitol salt agar mh 118- 100ghimedia , mercuric chloride paper , mercuric iodide ar , methanol hplc , methanol u v , mono ethanolamine ar , n heptane ar , n-butyl acetate ar , n-hexane ar , n-hexane hplc , nitricacid ar , orthophosphoric acid ar , oxidase disc- dd018-1vl himedia50 nos per vial , paraffin wax ar , penicillinase from bacillus cereus; sigma aldrich p.0389-1ku lyophilised powder 1500-3000 units / mg protein , perchloric acid ar , petroleum ether ar 40º - 60ºc , petroleum ether ar 60º-80ºc , phloroglucinol ar , polyhexanide ( vantocil ib ) ar , potassium chromate a r , potassium dichromate ar , potassium dihydrogen orthophosphate , potassium hydrogen phthalate volumetric std. traceable to nist.srm ( 1.02400.0080 ) merck millipore corporation , potassium hydroxide pellets ar , potassium iodide ar , potassium permanganate ar , salicylic acid emparta merck , saniocid sp surface & instrument disinfectant , silica gel f 254 ( merck silica gel 60 f254 ) ar , silica gel g ar ( 13%w / w caso4 1 / 2 h2o ) , silica gel gf- 254 ar ( g / uv 254 ) , silicon oil ar , silicon wax , sodiumhypochlorite solution ( availble chlorine 4-6% w / v ) ar , sodiumhypochlorite solution ( availble chlorine 3% w / v ) ar , sodiumhypochlorite solution ( availble chlorine 5% w / v ) a r , sodium acetate anhydrousa r , sodium acetate trihydrate ar , sodium arsenite ar , sodium bicarbonate ar , sodium bisulphate ar , sodium bisulphite ar , sodium borohydride sigmaaldrich 16940-66-2 ar , sodium carbonate ar anhydrous , sodium chloride ar , sodium dihydrogen orthophosphate ar , sodium fluoride ar , sodium hydroxide pellets arcdh , sodium lauryl sulpahte ar , sodium metabisulfite ar , sodium nitroprusside ar , sodium salicylate sigma aldrichemsure 1.06601 , sodium sulphate ar anhydrous , sodium sulphite ar , sodium thiosulphate pentahydrate ar , solochrome dark bluear , soyabean- casein digest media ( casein soyabean digest broth ) mh011-500g himedia , stannous chloride ar , starch iodide paper , starch soluble ar , steam indicator strip la811 himedia , sterile cotton swab himedia pw1136 , sucrose ar , sulphuric acid ar , ter butyl methyl ether ar , tetra butyl ammonium hydroxide 20% aqueous solution ar , tetra butyl ammonium hydroxide ar ( 0.1n in isopropanol ) , tetra butyl ammonium phosphate ar ( 1m solution ) , tetra methyl ammonium hydrogen sulphate hplc , tetradecyl ammonium bromide ar for hplc , tetrahydrofuran ar , tetrahydrofuran hplc / uv , thioglycolic acid ar80% , toluene ar , toluene hplc / u v grade , toluene rectified a r , tri sodium orthophosphate a r , triethylamine hplcgrade , tris buffer ar , tween 20 ar , xanthydrol ar , zinc chloride purifiedar , zinc metal ar granulated , zincon reagent ar