tender for supply of chemical/ consumable/ reagent and size , name of the chemical/ consumable/ reagent and size , antibiotic antimycotic solution 100x liquid, endotoxin tested w/10,000 u penicillin, 10mg streptomycin and 25 μg amphotericin b per ml in 0.9% normal saline 100 ml , molecular biology grade water 500 ml , β-tubulin monoclonal antibody 100 μl , 1,2,4-triazole 100 gms , 2-mercaptoethanol 100 ml , guanidine thiocyanate 25 gms , 100 bp dna ladder pack size : 50 ln , microcentrifuge tube 1.5 ml pack size: 1×500 nos. , agarose, ultrapure low eeo 50 gms , 10 x tae 500 ml , 10 x tbe 500 ml , ethidium bromide 5 g , 1 kb dna ladder 50 ln , 5 x sucrose gel loading dye 5 ml , tris hydrochloride 100 gms , edta disodium magnesium salt tetrahydrate 100 gms , sds 100 gms , glycine 500 gms , hydrochloric acid abt 35 % pure 500 ml , sodium hydroxide pellets 500 gms , pcr binding solution 30 ml , mgcl2 anhydrous 100 gms , 1 x pbs solution 100 ml , filter paper for blotting 25 nos , cryovials, externally threaded -2ml-48nos , hipura® ripa lysis buffer 100 ml , nonidet® p-40 100 ml , protease inhibitor cocktail 1 ml , phenylmethanesulfonyl fluoride (pmsf) 1 gm , hydrogen peroxide 100 ml , phosphatase inhibitor cocktail iii 1 ml , triton® x-100 100ml , syringe driven filters -pvdf hydrophilic membrane , pore size 0.22 μm ,25mm diameter 50 nos , trypsin-edta solution 1x w/ 0.05% trypsin and 0.02% edta in dulbeccos phosphate buffered saline w/o phenol red 100 ml , bradford reagent 100ml , folins reagent 125ml , acrylamide/bis acrylamide soln 30% (19:1), 100ml , ammonium persulphate 100 gms , temed 100ml , 10x tris-glycine-sds gel running buffer 500ml , 5x protein loading buffer (reducing) 5ml , 1m dtt 25 ml , prestained protein ladder 10ln , nitrocellulose membrane for blotting 20sh , 10x transfer buffer 500ml , hienviro-sponge™ stick hydrated w/10ml neutralizing buffer, sterile 5 ×5 nos , 10x tbst (tris buffered saline with tween-20® ) 1l , blocking buffer 100 ml , peptonized sm powder 500 gms , ecl substrate (femtoplus for western blotting) 50 ml , goat anti-rabbit igg antibody, peroxidase conjugated 2 ml , goat anti-mouse igg perooxidase conjugate 200 μg , β - actin polyclonal antibody 100 ul , gapdh polyclonal antibody 100 ul , ro60 polyclonal antibody 100 ul , orcinol monohydrate 25 gms , resorcinol 100 gms , orthophosphoric acid abt. 88% 500 ml , hidispo™ bag - 7 25o nos , bluple nitrile examination gloves, medium size 10 × 20 nos. , bluple nitrile examination gloves, large size 10 × 20 nos. , disposable masks 1 x 100 nos