procurement of digital pcr (droplet) under hadp project- 21, at division of fruit science, main campus shalimar, skuast-k. , procurement of digital pcr (droplet) under hadp project- 21, at division offruit science, main campus shalimar, skuast-k. , technical specifications , the digital pcr system is an all-in-one fully integrated system equipped with a partitioning module, thermal cycler, and images in a single system , digital pcr system should be equipped with a control software using a lcd touch monitor , system should able to use one plate with a multiplexing ability up to five channels, multiplexing up to 5 different dyes for single well without the need of customization of dyes or wavelength , system should offer below excitation and emission wavelength for light source and detection system , digital pcr should perform excitation and detection of up to 2 fluorescent dyes per partition (green, yellow). , system should be equipped with an independent reference channel for counting the number of filled partitions and determining the analyzable volume. , system should use high power white led for illumination and image acquisition is performed using cmos camera with 6.3 mp. , digital pcr system shouldanalyze a full plate under 2 – 3 hours , system should be compatible with probe-based detection chemistry and eva green chemistry, vendor should provide validated master mixes to be used on digital pcr system , digital pcr system should work with nano plate technology, should be compatible with 8.5k and 26k nanoplates, which has a total of 8,500 and/or 26,000 partitions. , nanoplate should be in standard sbs format. the 8.5k nanoplate holds a total of 96 reactions (96-well) and the 26k nanoplate up to 24 reactions (24-well format) with minimum 8 wells. , pcr well plate should have an input reaction volume: - 12 microliter and 40 microliter. , precision of ±10% should be achieved on the system , software should be accessed through a server network and also installed in individual computers for analysis purposes , system software should allow users to access all images acquired by the digital pcr system for each channel , software of the system should offer data visualization as a table, histogram, scatterplot, heatmap and bar chart, software should display fluorescence amplitude per partition in a 1d or 2d scatterplots (for multiplex data). , system software should computes concentration in copies/ul for each sample. , software shouldautomatically set thresholds across the entire plate or for individual wells. alternatively, the user can manually set the thresholds. , software should offer data analysis for the calculation of mutation frequency, gene expression fold change, copy number variation as well as genome editing , all analysis data and images in the software should exported as a .csv file (for use in microsoft excel or similar program) and .png or .svg (for images) and pdf format , software of the system should be with guidelines of 21 cfr, vendor should provide document for the same as and when required , number of installations:- vendor should have more than 20 installations in india. , warranty:- one year from date of the installation of the system , application training should be provided to two to three staff members by trained application team of the vendor / manufacturer , note:-the rates quoted are inclusive of all carriages of materials (mechanical/manual) from source to site work and all extra chargescomplete in all respects.