equipment , purchase of equipment , specifications for inverted fluorescent cell imager: 1) imaging channels: bright field channel and minimum 3 fluorescence channels (must include blue, green, and red) 2) illumination light sources – minimum 3 led cubes (must include for blue channel, for green channel, for red channel) 3) bright field channel: multiple green leds 4) excitation and emission spectra a) blue channel excitation: 355/40 nm; emission: 433/36 nm b) green channel excitation: 480/17 nm; emission: 517/23 nm c) red channel excitation: 556/20 nm; emission: 615/61 nm 5) all dyes and fluorescent proteins with compatible excitation and emission spectra should be used. 6) user interface: minimum 10.1 in. color (26 cm) touch-screen lcd monitor, with anti-glare and anti-fingerprint treatment 7) image resolution: minimum 1,280 x 768 pixel image resolution, 80–180° angle tilt range 8) focusing mechanism of coarse and fine, manual/automated adjustment 9) monochrome camera with 12 bit cmos, minimum 3.2 megapixels 10) data format; jpeg, tiff, or raw image files 11) image merging capability – images from up to 4 channels can be overlaid 12) data storage: minimum 4gb internal memory 13) data export: minimum 2 usb ports 14) display output: 1 hdmi port 15) objective of minimum 20x with numerical aperture 0.40 16) display magnification should be standard:minimum 100x and zoom with 700x 17) maximum imaging area: 0.70 mm2 field of view 18) motorized stage: minimum 6 mm travel in x, y direction, touch-screen or software control of travel speed and direction 19) compatibility – flasks: t25, t75, or t225 & multi-well plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates, petri dishes: 35mm, 60 mm, or 100 mm 20) slides: chamber slides or standard glass microscopy slides 21) integrated onboard software 22) stand-alone android operating system. 23) warranty: 1 year for the instrument.