procurement of supersignal west pico plus chemiluminescent substrate, etc procurement of supersignal west pico plus chemiluminescent substrate, etc , procurement of supersignal™ west pico plus chemiluminescent substrate, etc , supersignal™ west pico plus chemiluminescent substrate specifications: supersignal west pico plus chemiluminescent substrate characteristics include: • sensitivity: pictogram to femtogram sensitivity • stability: 8-hr working solution stability; 1-year kit stability at room temperature • compatibility: nitrocellulose and pvdf membranes • signal duration: 6 to 24 hours • recommended primary antibody concentration: 1:1, 000–1:5, 000 dilution ( 0.2–1.0 μg / ml ) • recommended secondary antibody concentration: 1:20, 000–100, 000 dilution ( 10–50 ng / ml ) • detection limit: 1 picogram ( pg ) , supersignal™ west femto maximum sensitivity substrate specifications: supersignal west femto maximum sensitivity substrate characteristics include: • sensitivity: low-femtogram ( mid-zeptomole ) • stability: 8-hr working solution stability, 1-year kit stability at 4°c • compatibility: nitrocellulose and pvdf membranes • signal duration: up to 8 hours • recommended primary antibody concentration: 1:5, 000–1:100, 000 dilution ( 10–200 ng / ml ) • recommended secondary antibody concentration: 1:100, 000–1:500, 000 dilution ( 2–10 ng / ml ) • detection limit: 60 femtogram ( fg ) , jc-1 dye ( mitochondrial membrane potential probe ) specifications: • materials: jc-1; cbic2 ( 3 ) • amount: 5 mg • concentration: 1.5 to 7.7mm • molecular weight: 652 • storage: 2 – 6 ? c ( dessicate and protect from light ) • stability: 6 – 12 months • excitation o monomer: 514 / 529nm o j-aggregate: 585 to 590nm o monomer and j-aggreate simultaneous: 475 ± 20nm applications: flow cytometry, fluorescent microscopy , packing & forwarding, transportation & insurance charges