Tender For Purchase Of Chemicals , Purchase Of Chemicals , Primescript™ 1St Strand Cdna Synthesis Kit Size: 50 Reactions Primescript™ 1St Strand Cdna Synthesis Kit Is Designed For Efficient Synthesis Of First-Strand Cdna From Total Rna Or Purified Mrna. T
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Tender For Purchase Of Chemicals , Purchase Of Chemicals , Primescript™ 1St Strand Cdna Synthesis Kit Size: 50 Reactions Primescript™ 1St Strand Cdna Synthesis Kit Is Designed For Efficient Synthesis Of First-Strand Cdna From Total Rna Or Purified Mrna. The Kit Contains Primescript Reverse Transcriptase, Oligo(Dt) Primer, Random Hexamer Primers, Rnase Inhibitor, And Optimized Reaction Components To Generate High-Yield, Full-Length Cdna Suitable For Conventional Pcr, Real-Time Pcr, Cloning, And Gene Expression Studies. , Rnaiso Plus Size: 200 Ml Rnaiso Plus Is A Monophasic Solution Of Phenol And Guanidinium Thiocyanate Formulated For Rapid Isolation Of High-Quality Total Rna From Cultured Cells, Tissues, And Other Biological Samples. The Reagent Efficiently Lyses Cells, Inactivates Rnases, And Enables The Recovery Of Intact Rna Suitable For Reverse Transcription, Real-Time Pcr, Northern Blotting, Rna Sequencing, And Other Downstream Molecular Biology Applications. , Mighty Ta-Cloning Kit Size: 20 Reactions The Mighty Ta-Cloning Kit Is A Ready-To-Use Cloning System Designed For Efficient Cloning Of Pcr Products Possessing 3-Adenine Overhangs Generated By Taq Dna Polymerase. The Kit Contains The Pmd20-T Cloning Vector, Ligation Reagents, And The Necessary Components For Rapid Ligation, Enabling High Cloning Efficiency For Routine Molecular Biology Applications. It Is Suitable For Downstream Sequencing, Subcloning, And Recombinant Dna Studies. , 100 Bp Dna Ladder (Dye Plus) Size: 500 Μl The 100 Bp Dna Ladder (Dye Plus) Is A Ready-To-Use Molecular Weight Marker Containing Dna Fragments Ranging From 100 Bp To 2,000 Bp. The Ladder Is Pre-Mixed With Loading Dye And Tracking Dyes, Allowing Direct Loading Onto Agarose Gels Without Additional Preparation. It Provides Accurate Estimation Of Pcr Product Sizes During Agarose Gel Electrophoresis. , Tb Green® Premix Ex Taq™ Ii (Tli Rnaseh Plus) Size: 200 Reactions Tb Green® Premix Ex Taq™ Ii (Tli Rnaseh Plus) Is A 2× Ready-To-Use Master Mix Formulated For Highly Sensitive Real-Time Pcr Using Tb Green Dye Chemistry. The Premix Contains Ex Taq Hs Dna Polymerase, Dntps, Mg²⁺, Optimized Reaction Buffer, And Tli Rnase H Plus, Ensuring High Specificity, Amplification Efficiency, And Reproducibility. It Is Compatible With Most Real-Time Pcr Platforms And Is Suitable For Quantitative Gene Expression Analysis. , Ribonuclease H (Rnase H) Size: 1000 Units Ribonuclease H (Rnase H) Is A Recombinant Enzyme That Specifically Hydrolyses The Rna Strand Of Rna-Dna Hybrids Without Degrading Single- Or Double-Stranded Dna. The Enzyme Is Supplied With An Optimized Reaction Buffer And Is Commonly Used Following First-Strand Cdna Synthesis To Remove Rna Templates, Thereby Improving The Efficiency Of Downstream Pcr And Other Molecular Biology Applications. , Emeraldamp® Max Pcr Master Mix Size: 160 Reactions Emeraldamp® Max Pcr Master Mix Is A 2× Pcr Master Mix Containing Dna Polymerase, Dntps, Mg²⁺, Optimized Reaction Buffer, And Electrophoresis Loading Dyes. The Formulation Enables Direct Loading Of Pcr Products Onto Agarose Gels Without The Addition Of Loading Buffer, Simplifying Workflow While Providing Robust Amplification For Routine Endpoint Pcr Applications. , The Protein Molecular Weight Marker (High) Size: 200 Lanes The Protein Molecular Weight Marker (High) Is An Unstained Protein Standard Developed For Accurate Molecular Weight Estimation During Sds-Page. It Consists Of Purified Protein Standards With Molecular Weights Ranging From 44.3 Kda To 200 Kda, Providing Well-Resolved Reference Bands For Analysis Of Medium- To High-Molecular-Weight Proteins. The Marker Is Supplied With 5× Protein Loading Buffer And 1 M Dtt For Convenient Sample Preparation And Produces Sharp, Uniformly Stained Bands Following Coomassie Brilliant Blue Staining. It Is Suitable For Routine Protein Electrophoresis, Recombinant Protein Analysis, Protein Expression Studies, And Molecular Weight Determination In Sds-Page Applications. , Takara La Taq® Size: 250 Units Takara La Taq® Is A High-Performance Dna Polymerase Developed Based On The Long And Accurate (La) Pcr Principle. The Enzyme Possesses Proofreading Activity Through A Combination Of Taq Dna Polymerase And A Proofreading Polymerase, Allowing Highly Accurate Amplification Of Long Dna Fragments. It Is Supplied With Optimized Mg²⁺ Reaction Buffer And Dntps, Making It Suitable For Amplification Of Genomic Dna, Gc-Rich Templates, And Long Pcr Products With High Fidelity And Efficiency. , 1 Kb Dna Ladder (Dye Plus) Size: 500 Μl The 1 Kb Dna Ladder (Dye Plus) Is A Ready-To-Use Molecular Weight Marker Containing Dna Fragments Covering A Broad Size Range Suitable For Analysis Of Pcr Products And Restriction Digests. The Ladder Is Pre-Mixed With Loading Dye And Tracking Dyes, Allowing Direct Loading Onto Agarose Gels Without Additional Preparation. It Provides Reliable Size Estimation And Clear Band Visualization During Electrophoresis. , Primescript™ Rt Reagent Kit Size: 200 Reactions Primescript™ Rt Reagent Kit (Perfect Real Time) Is Designed For Efficient First-Strand Cdna Synthesis For Two-Step Real-Time Rt-Pcr Applications. The Kit Contains Primescript Reverse Transcriptase, Optimized Reaction Buffer, Random Hexamers, Oligo(Dt) Primers, Rnase Inhibitor, And Dntps, Enabling Synthesis Of High-Quality Cdna From Total Rna Or Mrna. The Optimized Formulation Provides High Reverse Transcription Efficiency, Excellent Sensitivity, And Reproducibility For Quantitative Gene Expression Analysis. , Emeraldamp® Gt Pcr Master Mix Size: 160 Reactions Emeraldamp® Gt Pcr Master Mix Is A 2× Ready-To-Use Pcr Master Mix Containing A High-Performance Dna Polymerase, Dntps, Mg²⁺, Optimized Reaction Buffer, And Gel Loading Dyes. The Formulation Allows Pcr Products To Be Loaded Directly Onto Agarose Gels Without Adding Loading Buffer, Reducing Handling Time And Minimizing Contamination. It Provides Robust Amplification With High Specificity And Is Suitable For Routine Pcr Applications. , Primestar® Gxl Dna Polymerase Size: 250 Units Primestar® Gxl Dna Polymerase Is A High-Fidelity Dna Polymerase Engineered For Accurate Amplification Of Long Dna Fragments And Gc-Rich Templates. The Enzyme Combines The Proofreading Activity Of Primestar Hs Dna Polymerase With A Proprietary Extension Factor, Enabling Amplification Of Targets Up To 30 Kb From Genomic Dna While Maintaining Excellent Fidelity And Amplification Efficiency. It Is Supplied With Optimized Reaction Buffer And Dntps For Reliable Long-Range Pcr Applications. , Premix Ex Taq™ (Probe Qpcr) Size: 200 Reactions Premix Ex Taq™ (Probe Qpcr) Is A 2× Ready-To-Use Real-Time Pcr Master Mix Specifically Formulated For Dual-Labeled Probe-Based Quantitative Pcr Assays. The Premix Contains Takara Ex Taq Hs Dna Polymerase, Dntps, Mg²⁺, Optimized Reaction Buffer, Tli Rnase H, And Rox Reference Dyes, Providing High Amplification Efficiency, Excellent Specificity, And Reproducible Quantitative Detection. It Is Compatible With Most Real-Time Pcr Platforms And Supports Both Standard And Fast Cycling Protocols.
BOQ
| Sl. No. | Item Description |
| 1 | Purchase of Chemicals |
| 2 | PrimeScript™ 1st strand cDNA Synthesis Kit Size: 50 reactions PrimeScript™ 1st Strand cDNA Synthesis Kit is designed for efficient synthesis of first-strand cDNA from total RNA or purified mRNA. The kit contains PrimeScript Reverse Transcriptase, oligo(dT) primer, random hexamer primers, RNase inhibitor, and optimized reaction components to generate high-yield, full-length cDNA suitable for conventional PCR, real-time PCR, cloning, and gene expression studies. |
| 3 | RNAiso Plus Size: 200 mlRNAiso Plus is a monophasic solution of phenol and guanidinium thiocyanate formulated for rapid isolation of high-quality total RNA from cultured cells, tissues, and other biological samples. The reagent efficiently lyses cells, inactivates RNases, and enables the recovery of intact RNA suitable for reverse transcription, real-time PCR, Northern blotting, RNA sequencing, and other downstream molecular biology applications. |
| 4 | Mighty TA-cloning KitSize: 20 reactionsThe Mighty TA-cloning Kit is a ready-to-use cloning system designed for efficient cloning of PCR products possessing 3'-adenine overhangs generated by Taq DNA polymerase. The kit contains the pMD20-T cloning vector, ligation reagents, and the necessary components for rapid ligation, enabling high cloning efficiency for routine molecular biology applications. It is suitable for downstream sequencing, subcloning, and recombinant DNA studies. |
| 5 | 100 bp DNA Ladder (Dye Plus)Size: 500 µlThe 100 bp DNA Ladder (Dye Plus) is a ready-to-use molecular weight marker containing DNA fragments ranging from 100 bp to 2,000 bp. The ladder is pre-mixed with loading dye and tracking dyes, allowing direct loading onto agarose gels without additional preparation. It provides accurate estimation of PCR product sizes during agarose gel electrophoresis. |
| 6 | TB Green® Premix Ex Taq™ II (Tli RNaseH Plus)Size: 200 reactionsTB Green® Premix Ex Taq™ II (Tli RNaseH Plus) is a 2× ready-to-use master mix formulated for highly sensitive real-time PCR using TB Green dye chemistry. The premix contains Ex Taq HS DNA Polymerase, dNTPs, Mg²⁺, optimized reaction buffer, and Tli RNase H Plus, ensuring high specificity, amplification efficiency, and reproducibility. It is compatible with most real-time PCR platforms and is suitable for quantitative gene expression analysis. |
| 7 | Ribonuclease H (RNase H)Size: 1000 unitsRibonuclease H (RNase H) is a recombinant enzyme that specifically hydrolyses the RNA strand of RNA-DNA hybrids without degrading single- or double-stranded DNA. The enzyme is supplied with an optimized reaction buffer and is commonly used following first-strand cDNA synthesis to remove RNA templates, thereby improving the efficiency of downstream PCR and other molecular biology applications. |
| 8 | EmeraldAmp® Max PCR Master MixSize: 160 reactionsEmeraldAmp® Max PCR Master Mix is a 2× PCR master mix containing DNA polymerase, dNTPs, Mg²⁺, optimized reaction buffer, and electrophoresis loading dyes. The formulation enables direct loading of PCR products onto agarose gels without the addition of loading buffer, simplifying workflow while providing robust amplification for routine endpoint PCR applications. |
| 9 | The Protein Molecular Weight Marker (High)Size: 200 lanesThe Protein Molecular Weight Marker (High) is an unstained protein standard developed for accurate molecular weight estimation during SDS-PAGE. It consists of purified protein standards with molecular weights ranging from 44.3 kDa to 200 kDa, providing well-resolved reference bands for analysis of medium- to high-molecular-weight proteins. The marker is supplied with 5× protein loading buffer and 1 M DTT for convenient sample preparation and produces sharp, uniformly stained bands following Coomassie Brilliant Blue staining. It is suitable for routine protein electrophoresis, recombinant protein analysis, protein expression studies, and molecular weight determination in SDS-PAGE applications. |
| 10 | TaKaRa LA Taq®Size: 250 unitsTaKaRa LA Taq® is a high-performance DNA polymerase developed based on the Long and Accurate (LA) PCR principle. The enzyme possesses proofreading activity through a combination of Taq DNA polymerase and a proofreading polymerase, allowing highly accurate amplification of long DNA fragments. It is supplied with optimized Mg²⁺ reaction buffer and dNTPs, making it suitable for amplification of genomic DNA, GC-rich templates, and long PCR products with high fidelity and efficiency. |
| 11 | 1 kb DNA Ladder (Dye Plus)Size: 500 µlThe 1 kb DNA Ladder (Dye Plus) is a ready-to-use molecular weight marker containing DNA fragments covering a broad size range suitable for analysis of PCR products and restriction digests. The ladder is pre-mixed with loading dye and tracking dyes, allowing direct loading onto agarose gels without additional preparation. It provides reliable size estimation and clear band visualization during electrophoresis. |
| 12 | PrimeScript™ RT Reagent KitSize: 200 reactionsPrimeScript™ RT Reagent Kit (Perfect Real Time) is designed for efficient first-strand cDNA synthesis for two-step real-time RT-PCR applications. The kit contains PrimeScript Reverse Transcriptase, optimized reaction buffer, random hexamers, oligo(dT) primers, RNase inhibitor, and dNTPs, enabling synthesis of high-quality cDNA from total RNA or mRNA. The optimized formulation provides high reverse transcription efficiency, excellent sensitivity, and reproducibility for quantitative gene expression analysis. |
| 13 | EmeraldAmp® GT PCR Master MixSize: 160 reactionsEmeraldAmp® GT PCR Master Mix is a 2× ready-to-use PCR master mix containing a high-performance DNA polymerase, dNTPs, Mg²⁺, optimized reaction buffer, and gel loading dyes. The formulation allows PCR products to be loaded directly onto agarose gels without adding loading buffer, reducing handling time and minimizing contamination. It provides robust amplification with high specificity and is suitable for routine PCR applications. |
| 14 | PrimeSTAR® GXL DNA PolymeraseSize: 250 unitsPrimeSTAR® GXL DNA Polymerase is a high-fidelity DNA polymerase engineered for accurate amplification of long DNA fragments and GC-rich templates. The enzyme combines the proofreading activity of PrimeSTAR HS DNA Polymerase with a proprietary extension factor, enabling amplification of targets up to 30 kb from genomic DNA while maintaining excellent fidelity and amplification efficiency. It is supplied with optimized reaction buffer and dNTPs for reliable long-range PCR applications. |
| 15 | Premix Ex Taq™ (Probe qPCR)Size: 200 reactionsPremix Ex Taq™ (Probe qPCR) is a 2× ready-to-use real-time PCR master mix specifically formulated for dual-labeled probe-based quantitative PCR assays. The premix contains TaKaRa Ex Taq HS DNA Polymerase, dNTPs, Mg²⁺, optimized reaction buffer, Tli RNase H, and ROX reference dyes, providing high amplification efficiency, excellent specificity, and reproducible quantitative detection. It is compatible with most real-time PCR platforms and supports both standard and fast cycling protocols. Please Enable Macros to View BoQ information |
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