Science and Technology Government Departments Closing in 16 days TDR #56338081

Bids Are invited for Consumables For Western Blotting

Issued by Government Departments · Mumbai Suburban, Maharashtra
Tender Value
Ref. Documents
Estimated cost
Bid Submission
28 Jul 2026
16 days left
EMD
Ref. Documents
Bank guarantee accepted
Document Fee
Ref. Documents
Non-refundable
Tender Type
Gem

Tender Overview

Competition Type
NCB
Bidding Type
Tender
Location / State
Mumbai Suburban → Maharashtra
EMD Exemption
Available
Quantity
20

Project Description

Bids Are invited for Consumables For Western Blotting Protease And Phosphatase Inhibitor Cocktail - Protease And Phosphatase Inhibitor Cocktail Non Freezing Active Formulation Must Strictly Include Aebsf Aprotinin Bestatin Hydrochloride E 64 Leupeptin Hemisulfate Pepstatin A Cantharidin Dash Pbromolevamisole Oxalate And Calyculin A Pack Size 1Ml Detailed Specifications As Per Attached Annexure 1 , Ripa Radio Immunoprecipitation Assay Buffer - Ripa Radio Immunoprecipitation Assay Buffer Formulation Must Precisely Contain 50 Mm Tris Hcl Ph 7Point 8 8Point2 At 25 Degree C 150 Mm Nacl 1 Dot 0Percentage Non Ionic Detergent Igepal Ca 630 Or Exact Np 40 Equivalent 0 Dot 5Percentage Sodium Deoxycholate And 0 Dot 1Percentage Sds Dot Pack Size 50 Ml Detailed Specifications As Per Attached Annexure 1 Dot , Blasticidin S Ready Made Sterile Filtered Solution - Blasticidin S Ready Made Sterile Filtered Solution Pack Size 1 Ml Formulation Must Strictly Consist Of 10 Mg Per Ml Blasticidin S Hydrochloride In 20 Mm Hepes Buffer Detailed Specifications As Per Attached Annexure 1 , Broad Spectrum Phosphatase Inhibitor Cocktail - Broad Spectrum Phosphatase Inhibitor Cocktail Exactly One Tablet Should Be Sufficient To Treat 10 Ml Of Cell Lysate Or Extraction Buffer Without Requiring Weighing Or Aliquoting Pack Size 20 Tablets Per Pack Detailed Specifications As Per Attached Annexure 1 , Activated Sodium Orthovanadate Solution - Activated Sodium Orthovanadate Solution Must Be Strictly Supplied At A Concentration Of 10 Mg Per Ml In Aqueous Naoh Specifically Adjusted To Ph 10 Point 0 To Guarantee The Maintenance Of The Monomeric Highly Active Inhibitor State Pack Size 5 Ml Detailed Specifications As Per Attached Annexure 1 , Ponceau S Solution - Ponceau S Solution 0 Dot 1 Percentage W By V In 5 Percentage Acetic Acid Staining Action Must Be Completely Reversible Allowing Rapid Destaining With Water Or Standard Wash Buffers Like Tbst Pbst Without Altering Protein Antigenicity Or Interfering With Downstream Immunological Detection Pack Size 1L Detailed Specifications As Per Attached Annexure 1 , Hydrophobic Pvdf Transfer Membrane Roll - Hydrophobic Pvdf Transfer Membrane Roll 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Fully Compatible With Multiple Downstream Immunodetection Methods Including Chemiluminescence And Chromogenic Colorimetric Detection Detailed Specifications As Per Attached Annexure 1 , Pvdf Transfer Membrane - Pvdf Transfer Membrane 0 Dot 22 Micro M Pore Size 8 Dot 5 Cm X 10 M Roll Must Be Explicitly Validated For High Resolution Western Blotting And Be Fully Compatible With Downstream Protein Sequencing Applications Eg N Dash Terminal Degradation Detailed Specifications As Per Attached Annexure 1 , Aqueous Wetting Pvdf Transfer Membrane - Aqueous Wetting Pvdf Transfer Membrane 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Modified Polyvinylidene Fluoride Pvdf Transfer Membrane Engineered To Wet Out Directly In Aqueous Transfer Buffers Without Requiring A Prior Alcohol Detailed Specifications As Per Attached Annexure 1 , Pvdf Transfer Membrane For Fluorescent Western Blotting - Pvdf Transfer Membrane For Fluorescent Western Blotting 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Hydrophobic Polyvinylidene Fluoride Pvdf Membrane Specifically Optimized For Quantitative Fluorescent Western Blotting Applications Detailed Specifications As Per Attached Annexure 1 Total Quantity : 20

BOQ

Sl. No. Item TitleItem Description
1Protease and Phosphatase Inhibitor CocktailProtease and Phosphatase Inhibitor Cocktail Non freezing Active formulation must strictly include AEBSF Aprotinin Bestatin hydrochloride E 64 Leupeptin hemisulfate Pepstatin A Cantharidin dash pBromolevamisole oxalate and Calyculin A Pack size 1ml Detailed specifications as per attached annexure 1
2RIPA Radio Immunoprecipitation Assay BufferRIPA Radio Immunoprecipitation Assay Buffer Formulation must precisely contain 50 mM Tris HCl pH 7point 8 8point2 at 25 degree C 150 mM NaCl 1 dot 0percentage non ionic detergent IGEPAL CA 630 or exact NP 40 equivalent 0 dot 5percentage sodium deoxycholate and 0 dot 1percentage SDS dot Pack size 50 ml Detailed specifications as per attached annexure 1 dot
3Blasticidin S Ready Made sterile filtered SolutionBlasticidin S Ready Made sterile filtered Solution Pack size 1 ml Formulation must strictly consist of 10 mg per mL Blasticidin S hydrochloride in 20 mM HEPES buffer Detailed specifications as per attached annexure 1
4Broad Spectrum Phosphatase Inhibitor CocktailBroad Spectrum Phosphatase Inhibitor Cocktail Exactly one tablet should be sufficient to treat 10 mL of cell lysate or extraction buffer without requiring weighing or aliquoting Pack size 20 tablets per pack Detailed specifications as per attached annexure 1
5Activated Sodium Orthovanadate SolutionActivated Sodium Orthovanadate Solution Must be strictly supplied at a concentration of 10 mg per ml in aqueous NaOH specifically adjusted to pH 10 point 0 to guarantee the maintenance of the monomeric highly active inhibitor state Pack size 5 ml Detailed specifications as per attached annexure 1
6Ponceau S SolutionPonceau S Solution 0 dot 1 percentage w by v in 5 percentage Acetic Acid Staining action must be completely reversible allowing rapid destaining with water or standard wash buffers like TBST PBST without altering protein antigenicity or interfering with downstream immunological detection Pack size 1L Detailed specifications as per attached annexure 1
7Hydrophobic PVDF Transfer Membrane RollHydrophobic PVDF Transfer Membrane Roll 0 dot 45 micro m nominal pore size 8 dot 5 cm x 10 m roll Fully compatible with multiple downstream immunodetection methods including chemiluminescence and chromogenic colorimetric detection Detailed specifications as per attached annexure 1
8PVDF Transfer MembranePVDF Transfer Membrane 0 dot 22 micro m pore size 8 dot 5 cm x 10 m roll Must be explicitly validated for high resolution Western blotting and be fully compatible with downstream protein sequencing applications eg N dash terminal degradation Detailed specifications as per attached annexure 1
9Aqueous Wetting PVDF Transfer MembraneAqueous Wetting PVDF Transfer Membrane 0 dot 45 micro m nominal pore size 8 dot 5 cm x 10 m roll Modified Polyvinylidene fluoride PVDF transfer membrane engineered to wet out directly in aqueous transfer buffers without requiring a prior alcohol Detailed specifications as per attached annexure 1
10PVDF Transfer Membrane for Fluorescent Western BlottingPVDF Transfer Membrane for Fluorescent Western Blotting 0 dot 45 micro m nominal pore size 8 dot 5 cm x 10 m roll Hydrophobic Polyvinylidene fluoride PVDF membrane specifically optimized for quantitative fluorescent Western blotting applications Detailed specifications as per attached annexure 1

AI Tender Summary

OUR REF NO 56338081
AUTHORITY Government Departments
TENDER VALUE Ref. Documents
LAST DATE 28-07-2026
Authority
Authority Name Directorate Of Purchase And Stores
Work Description Bids Are invited for Consumables For Western Blotting Protease And Phosphatase Inhibitor Cocktail - Protease And Phosphatase Inhibitor Cocktail Non Freezing Active Formulation Must Strictly Include Aebsf Aprotinin Bestatin Hydrochloride E 64 Leupeptin Hemisulfate Pepstatin A Cantharidin Dash Pbromolevamisole Oxalate And Calyculin A Pack Size 1Ml Detailed Specifications As Per Attached Annexure 1 , Ripa Radio Immunoprecipitation Assay Buffer - Ripa Radio Immunoprecipitation Assay Buffer Formulation Must Precisely Contain 50 Mm Tris Hcl Ph 7Point 8 8Point2 At 25 Degree C 150 Mm Nacl 1 Dot 0Percentage Non Ionic Detergent Igepal Ca 630 Or Exact Np 40 Equivalent 0 Dot 5Percentage Sodium Deoxycholate And 0 Dot 1Percentage Sds Dot Pack Size 50 Ml Detailed Specifications As Per Attached Annexure 1 Dot , Blasticidin S Ready Made Sterile Filtered Solution - Blasticidin S Ready Made Sterile Filtered Solution Pack Size 1 Ml Formulation Must Strictly Consist Of 10 Mg Per Ml Blasticidin S Hydrochloride In 20 Mm Hepes Buffer Detailed Specifications As Per Attached Annexure 1 , Broad Spectrum Phosphatase Inhibitor Cocktail - Broad Spectrum Phosphatase Inhibitor Cocktail Exactly One Tablet Should Be Sufficient To Treat 10 Ml Of Cell Lysate Or Extraction Buffer Without Requiring Weighing Or Aliquoting Pack Size 20 Tablets Per Pack Detailed Specifications As Per Attached Annexure 1 , Activated Sodium Orthovanadate Solution - Activated Sodium Orthovanadate Solution Must Be Strictly Supplied At A Concentration Of 10 Mg Per Ml In Aqueous Naoh Specifically Adjusted To Ph 10 Point 0 To Guarantee The Maintenance Of The Monomeric Highly Active Inhibitor State Pack Size 5 Ml Detailed Specifications As Per Attached Annexure 1 , Ponceau S Solution - Ponceau S Solution 0 Dot 1 Percentage W By V In 5 Percentage Acetic Acid Staining Action Must Be Completely Reversible Allowing Rapid Destaining With Water Or Standard Wash Buffers Like Tbst Pbst Without Altering Protein Antigenicity Or Interfering With Downstream Immunological Detection Pack Size 1L Detailed Specifications As Per Attached Annexure 1 , Hydrophobic Pvdf Transfer Membrane Roll - Hydrophobic Pvdf Transfer Membrane Roll 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Fully Compatible With Multiple Downstream Immunodetection Methods Including Chemiluminescence And Chromogenic Colorimetric Detection Detailed Specifications As Per Attached Annexure 1 , Pvdf Transfer Membrane - Pvdf Transfer Membrane 0 Dot 22 Micro M Pore Size 8 Dot 5 Cm X 10 M Roll Must Be Explicitly Validated For High Resolution Western Blotting And Be Fully Compatible With Downstream Protein Sequencing Applications Eg N Dash Terminal Degradation Detailed Specifications As Per Attached Annexure 1 , Aqueous Wetting Pvdf Transfer Membrane - Aqueous Wetting Pvdf Transfer Membrane 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Modified Polyvinylidene Fluoride Pvdf Transfer Membrane Engineered To Wet Out Directly In Aqueous Transfer Buffers Without Requiring A Prior Alcohol Detailed Specifications As Per Attached Annexure 1 , Pvdf Transfer Membrane For Fluorescent Western Blotting - Pvdf Transfer Membrane For Fluorescent Western Blotting 0 Dot 45 Micro M Nominal Pore Size 8 Dot 5 Cm X 10 M Roll Hydrophobic Polyvinylidene Fluoride Pvdf Membrane Specifically Optimized For Quantitative Fluorescent Western Blotting Applications Detailed Specifications As Per Attached Annexure 1 Total Quantity : 20
Basic Detail
Tender No GEM/2026/B/7711231
Bidding Type Tender
Location
City Mumbai Suburban
State Maharashtra
Key Dates
Publish Date 07 Jul 2026
Submission Date 28 Jul 2026
Open Date 28 Jul 2026
Finance
Tender Value Ref. Documents
Tender Fee Ref. Documents
EMD Ref. Documents
Exemption Available
Document List
9b272313-bbc4-4f7f-baef-710026a66a2c.pdf
9520699_Boq_1.xlsx
mergedtechspec_2026-06-25-13-21-06_1bccddff22a822b8b8915f9e1d1783d8.pdf
pbdandhin_97f67968-9057-4f22-a5921782379043963_apod4.pdf

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Tender Timeline

Jul 07, 2026
11:30 IST

Tender Published

Tender notice published.

Completed
Jul 28, 2026
17:00 IST

Bid Submission Deadline

Online submission via eProcurement portal.

Upcoming · 16 days
Jul 28, 2026

Bid Opening Date

Technical bids will be opened and evaluated.

Upcoming · 16 days

Tender Documents

Download All (ZIP) ↓
pdf

9b272313-bbc4-4f7f-baef-710026a66a2c.pdf

xlsx

9520699_Boq_1.xlsx

pdf

mergedtechspec_2026-06-25-13-21-06_1bccddff22a822b8b8915f9e1d1783d8.pdf

pdf

pbdandhin_97f67968-9057-4f22-a5921782379043963_apod4.pdf

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