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| 1 | Item Description Name of the Item (Pack size wherever mentioned should strictly adherence the other pack size will not be entertained) Rate to be quoted only for the First quality |
| 2 | Real time PCR mastermix for DNA viruses • Universal qPCR master mix compatible with TaqMan probes at 2X concentration• Should have hot Start Taq DNA Polymerase• Nuclease free water and necessary sample buffers should be provided• Sample must be given for a minimum of 50 reactions |
| 3 | One step real time PCR mastermix for RNA viruses• One step Reverse Transcription real time PCR master mix (5x) compatible with TaqMan probes (Multiplexing)• RT enzyme• High fidelity hot Start Taq DNA Polymerase• Nuclease free water and necessary sample buffers should be provided• Sample must be given for a minimum of 50 reactions |
| 4 | One step real time PCR mastermix for RNA viruses• One step Reverse Transcription real time PCR master mix (2x) compatible with TaqMan probes, *RT enzyme, *hot Start Taq DNA Polymerase *Nuclease free water and necessary sample buffers should be provided• Sample must be given for a minimum of 50 reactions |
| 5 | cDNA synthesis kit • First strand cDNA synthesis kit containing Reverse Transcriptase• RNase Inhibitor• dNTP mix• Oligo dT primer• random hexamer primer, and buffer |
| 6 | Hotstart conventional PCR mastermix• Conventional hot start PCR master mix• should be colored- ready to load directly from PCR tube to gel• Sample must be given for a minimum of 50 reactions |
| 7 | PCR product gel purification kit (spin column)• Spin column-based PCR gel purification kit with necessary buffers |
| 8 | PCR product purification kit (spin column)• Spin column-based direct PCR product purification kit with necessary buffers |
| 9 | Molecular grade Agarose for gel electrophoresis• Molecular grade • Low EEO |
| 10 | Tris Acetate EDTA buffer (10x) for gel electrophoresis• Application: Electrophoresis |
| 11 | Tris EDTA buffer (1x), RNAse, DNase free molecular grade 100 ml• Molecular grade • DNase, RNase-free• ready to use• for reconstitution of primers |
| 12 | Nucleic acid Extraction plasticware compatible for ILS GENfast automated nucleic acid extraction machine (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb• Nucleic acid extraction• must provide compatibility certificate from OEM• U bottom• Sample must be given for a minimum of 3*96 reactions |
| 13 | Real time PCR plates-COBAS Z480• qPCR plates: Opaque• semi skirted, Low profile• 0.1ml volume• compatible with COBAS Z480 machine and CFX96• should be provided with clear sealing film for real time PCR applications• should be DNase, RNase, and pyrogen free• must produce compatibility certificate from OEM• should provide 5 sample plates |
| 14 | Real time PCR plates- QS5 • qPCR plates: Ultra clear, * semi skirted, regular profile * 0.2ml volume, * compatible with QS5, *should be provided with clear sealing film for real time PCR applications * must produce compatibility certificate from OEM * should be DNase, RNase, and pyrogen free• Sample should be given for 5 plates |
| 15 | Real time PCR strips-COBAS Z480• qPCR strips: opaque, low profile * 8well per strip, * must provide transparent strip caps * compatible with COBAS Z480 real time PCR and CFX 96* must produce certificate from OEM* should be DNase, RNase, and pyrogen free• Sample should be given for 5 strips |
| 16 | Real time PCR strips –QS5 • qPCR strips: Ultra clear, regular profile • 8well per strip• attached with transparent caps• compatible with QS5 real time PCR• must produce certificate from OEM• should be DNase, RNase, and pyrogen free• Sample should be given for 5 strips |
| 17 | Sanger sequencing services • Sequencing service for 1 sample includes PCR product purification, both forward and reverse strand sequencing |
| 18 | 100ul Filter tips• Filter tips 100ul universal tips, Must be compatible with standard pipettes brands including Eppendorf, Thermofisher, Gilson and tarson. |
| 19 | 10ul Filter tips• Filter tips 10-20ul universal tips, Must be compatible with standard pipettes brands including Eppendorf, Thermofisher, Gilson and tarson. |
| 20 | HEPES buffer • 1 M cell culture grade, liquid. |
| 21 | Sterile cell culture pipette (5 ml)• Sterile, RNase-/DNase-free, Nonpyrogenic, Noncytotoxic, Exclusive anti-drip tip assures accurate delivery.• Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier.• Bidirectional graduations provide choice of ascending and descending scales.• Negative graduations allow additional working volume.• Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic• Volumetric accuracy of ±2% at stated capacity• should provide 5 sample |
| 22 | Sterile cell culture pipette (10 ml) • Sterile, RNase-/DNase-free, Nonpyrogenic, Noncytotoxic, Exclusive anti-drip tip assures accurate delivery.• Ideal for sterile tissue culture applications. Color-coded magnifier stripes make volume reading and size selection easier.• Bidirectional graduations provide choice of ascending and descending scales.• Negative graduations allow additional working volume.• Packaging options: - Individually wrapped, paper/plastic - Individually wrapped, clear plastic• Volumetric accuracy of ±2% at stated capacity• should provide 5 sample plates |
| 23 | Cell culture medium EMEM • Powdered media of Eagle\"s minimum essential medium (EMEM) L-Glutamine Without Sodium bicarbonate and Sodium pyruvate. Ready to reconstitute in 1 liter of distilled water.• should provide 1 sachet as sample |
| 24 | Cell culture medium DMEM• Powdered media of Dulbecco\"s Modified Eagle Medium (DMEM) With 4.5gms Glucose per litre and L-Glutamine Without Sodium bicarbonate and Sodium pyruvate. Ready to reconsitute in 1 liter of distilled water.• should provide 1 sachet as sample |
| 25 | Antibiotic, antimycotic solution (20 ml pack)• Antibiotic Antimycotic Solution 100X liquid• w/10,000 U Penicillin, 10mg Streptomycin and 25 µg Amphotericin B per ml in 0.9% normal saline• Sterile filtered, suitable for cell culture. Exdotoxin tested.• should provide 1 pack as sample |
| 26 | Anti Mycoplasma spray• Quick and efficient elimination of mycoplasma contamination from cell cultures. • Retention of normal cell characteristics |
| 27 | Sterile Cell Scrapers• (With rotatable blade) Pivoting blades- 1.25cm Sterilized by gamma irradiation, Non-pyrogenic, non-toxic, DNase-Rnase (individually wrapped)• should provide 5 scrapers as sample |
| 28 | 96-Well Flat-Bottom Plate• Tissue Culture, polystyrene, lid (low evaporation) Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped)• should provide 5 plates as sample |
| 29 | 6-Well Flat-Bottom Plate• 6-Well Flat-Bottom Plate, Tissue Culture- Clear polystyrene flat-bottom, tissue culture-treated multiwell plate with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped)• should provide 5 plates sample |
| 30 | 12 Well plate Flat-bottom plate• 12 well plate, flat bottom, polystyrene, sterile, with lid. Non-pyrogenic, Sterile, RNase-/DNase-free. (individually wrapped)• should provide 5 plates sample |
| 31 | T-25 flask• Flask: optically clear polystyrene Cap: non-vented high-density polyethylene, Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 25 cm². Recommended working volume of 5-7 mL. Sterility by Gamma irradiation• should provide 5 flask sample |
| 32 | T-75 flask• Flask: optically clear polystyrene Cap: non-vented high-density polyethylene, Growth area is tissue culture-treated for optimal cell attachment Non-pyrogenic, Sterile, RNase-/DNase-free, Neck Style: angled. Approximate cell growth area of 75 cm². Recommended working volume of 15 - 22.5 mL. Sterility by Gamma irradiation• should provide 5 flask sample |
| 33 | Trypsin-EDTA (0.25%), with phenol red (100 ml pack)• Liquid formulation of trypsin contains EDTA and phenol red. Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination. |
| 34 | Trypsin, TPCK Treated (25 ug/vial)• Form-Powder, cell culture grade. |
| 35 | V-bottom micro titre plate • V-bottom clear wells, polystyrene, sterile, Nonpyrogenic, Noncytotoxic, with lid, packed individually.• should provide 5 plate sample |
| 36 | Conical Tubes (50 ml ) sterile• Non- skirted, Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF.• should provide 5 tubes sample |
| 37 | Conical Tubes (15 ml ) sterile • Hydrophobic, ,Leak-proof , biologically inert surface for good cell or protein recovery, graduations in 5mL increments, White writing area resists solvents, Irradiated for sterility, Resistant to alcohols and mild organic solvents, translucent wall for easy viewing. Stable from -80°C to +121°C (-112°F to +250°F), Withstand centrifugal forces to 16,000 x g RCF.• should provide 5 tubes sample |
| 38 | Cell Freezing Medium-DMSO, 1X Without Antibiotics Sterile filtered, 50 ml pack• For Cell preservation in liquid nitrogen |
| 39 | Autoclavable transparent biohazard disposal bags- small size • Clear transparent autoclavable bags• Approximately 12 • 10 inches |
| 40 | Kim wipes- lint free tissue • Single ply light duty wiper • For cleaning equipments • Must be lint free• Non abrasive |
| 41 | Blotting sheet• Purpose: General lab use• Color: White/grey• Size: 60 60cm• Pack size: 100 sheets min |
| 42 | Reagent reservoir • capacity 75ml, material polypropelene, autoclavable, without divider |
| 43 | Cryo Tags• 32.5 x 12.7• labels withstand conventional and cryogenic• storage conditions.Freezer temperatures down to -196°C (liquid and vapor phase nitrogen), boiling water baths (100°C), autoclaving and moderate oven temperature (150°C). They will resist water soaking for at least 24 hours at room temperature and water vapor (100% relative humidity) at 35°C for at least 24 hours. |
| 44 | sterile syringe filter• filter with a 0.45 µm pore size, 33 mm, gamma sterilized individually packed PVDF material• should provide 5 filters as sample |
| 45 | Fetal Bovine serum• Research Grade, Sterile Filtered, Heat Inactivated ,Origin- South American EU approved |
| 46 | Card Board Cryo Box • Capacity - 25 places, holding place for 1.8ml cryo vials, withstand -80 temperature at ultra freezing storage condition• Suitable for air cargo shipment |
| 47 | Nitrile Gloves -Small• Size: Small, Made of Nitrile, should be soft and comfortable to wear• should provide 5 pair as sample |
| 48 | Nitrile Gloves medium• Size: Medium, Made of Nitrile, should be soft and comfortable to wear• should provide 5 pair as sample |
| 49 | Nitrile Gloves Large • Size: Large, Made of Nitrile, should be soft and comfortable to wear• should provide 5 pair as sample |
| 50 | Serum sample cryogenic vials • Capacity - 0.5 ml *made of polypropylene material, self stand Screw capped vial with silicon O ring, leak proof, sterile, DNase, RNase, Pyrogen free, Autoclavable |
| 51 | Iso-propanol• Density: 786 kg/m³• Boiling point: 82.3 °C• Molar mass: 60.1 g/mol• IUPAC ID: propan-2-ol• molecular grade |
| 52 | Tissue roll• 2 ply Tissue rolls• 15 GSM |
| 53 | Autoclavable transparent biohazard disposal bags- big size • Clear transparent autoclavable bags big one • Approximatelyinches 24 X 30 |
| 54 | 11% hydrogen peroxide stabilized with silver suitable for fogging sterilization• stabilized with silver suitable for fogging sterilization |
| 55 | Mixed cellulose esters (MCE)• membrane plain filter, • 13 mm diameter,• hydrophilic,• white,• non-sterile • 0.22um• 3 sample must be provided |
| 56 | Membrane filter Holder-47mm • 47mm Material PSF, • upper chamber 500ml, • lower chamber 1000ml, • Autoclavable |
| 57 | Sterile Pasteur pipettes• Made from medical grade, lab quality, high-quality Low-Density Polyethylene (LDPE). • Individually wrapped in a thermoformed Paper/Plastic peel pack to maintain sterility. • Gamma sterile. • Graduated markings to provide good clarity for easy observation of liquid levels. |
| 58 | Sodium bicarbonate powder• BioReagent, Molecular Biology.• suitable for cell culture.• suitable for insect cell culture.• From Merck/Sigma-Aldrich. |
| 59 | 100bp DNA Ladder• Consisting of 12 double stranded DNA fragments ranging from 100bp to 3000bp in increments.• Ready-to-load ladders.• Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes. |
| 60 | 500bp DNA Ladder• Ready-to-load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. • Consisting of 10 linear double-stranded fragments. • Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes. |
| 61 | Cryocane• Constructed from lightweight and durable aluminum for long-term use. • Ideal for holding cryo vials in liquid nitrogen storage tanks. • Designed to function smoothly without damage at cryogenic temperatures. ensuring safe and efficient storage. |
| 62 | Biological Indicator for Steam Autoclave• Provides BI results in 24 minutes. • Dynamic-air-removal (Pre-vacuum and SFPP): 132˚C (270˚F) 3 or 4 min. 135˚C (275˚F) 3 min.• Meets ISO and FDA performance requirements for biological indicators.• Contains viable organisms that provide a direct measure of lethality.• Puts your instrument turnaround speed into overdrive. |
| 63 | Membrane Filters 0.2um, 47mm• MCE Membrane Filter, 0.22 μm Pore Size.• filter diam. 47 mm, hydrophilic, white.• Mixed Cellulose Ester membrane filter discs. |
| 64 | Optical Plate Seals• Non-pierceable but can be easily removed after the PCR reaction is performed.• A pressure applied by the heated lid of a thermal cycler is needed to keep the seal well closed during thermal cycling. • Has excellent optical properties.• Temperature range -20°C to 120°C |
