Quotations For Purchase Of Consumables For The Project Work Of Dr. Jessy Abraham, Additional Professor Of Biochemistry At Aiims, Raipur

Sharing tender

Quotations For Purchase Of Consumables For The Project Work Of Dr. Jessy Abraham, Additional Professor Of Biochemistry At Aiims, Raipur--1 Microtips 100-1000uL with references medical grade polypropylene autoclavable, free of lubricants, dyes and heavy metals. Certified DNase, RNase, Human DNA and PCR inhibitor free. Sterile Tips are Pyrogen Free. (500p/Pack) 2 Microtips 20-200uL with references medical grade polypropylene autoclavable, free of lubricants, dyes and heavy metals. Certified DNase, RNase, Human DNA and PCR inhibitor free. Sterile Tips are Pyrogen Free. (1000p/Pack) 3 Filtered Microtips 10uL XL Filter tips protect the sample from aerosols and liquids. A proper barrier to all contaminants, the tips come with reliable liquid blocking technology. Free of detectable DNase, RNase, Human DNA, PCR inhibitor free, Sterile variety free of pyrogen (1000p/Pack) 4 Non-Filtered Microtips 10uL XL Filter tips protect the sample from aerosols and liquids. A proper barrier to all contaminants, the tips come with reliable liquid blocking technology. Free of detectable DNase, RNase, Human DNA, PCR inhibitor free, Sterile variety free of pyrogen (1000p/Pack) 5 Micro Centrifuge Tube 1.5mL. The tubes handle small amounts of liquid and are designed for storage, spinning down, as reaction vessels, or for separating samples. Required superior quality and manufactured without slip agents, PP material confirming to US FDA 21 CFR Autoclavable (500p/Pack) 6 Micro Centrifuge Tube 2.0mL. The tubes handle small amounts of liquid and are designed for storage, spinning down, as reaction vessels, or for separating samples. Required superior quality and manufactured without slip agents, PP material confirming to US FDA 21 CFR Autoclavable (500p/Pack) 7 Tissue wipes (Size: 11.1CM X 21.3CM) Delicate Task Wipes Easily wipe up liquid and dust. The anti-static dispensing reduces lint and electrostatic discharge and controls usage. Wipers are specifically designed to gently clean liquid and dust and are great for instrumentation, surface, parts and lens cleaning. It has to be designed to reduce lint and low extractables (280p/Pack) 8 Ethanol for molecular biology with molecular biology grade should be suitable for a broad spectrum of classical lab applications and also for the extraction protocols at the nucliec acids. It should be free from all the impurities at the purest form of 99.9% absolute alcohol. (500mL) 9 0.1 ml Low Profile Tube Strips with Cap suitable for 0.1 ml thermal blocks. Only Ultra Clear variety suitable for REAL TIME PCR Low profile reduces dead space between heated lid and sample. Thin wall design for even heat transfer. Clean room production for consistent results. High quality amplification. Suitable for traditional PCR/ Hot start PCR. Material:PP (125p/Pack) 10 Cryo Box Plastic 81Places Stores vials at the lowest of temperatures. Designed in a way to arrange,orient, identify and access vials with ease. Material: PC Autoclavable (4p/Pack) 11 The SYBR green for qPCR 500Rxn, the reagent should be specifically designed for intercalator-based real time PCR. It should be supplied at a 2X concentration premixed at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent should also contain RNase H, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. The product, with a modified buffer composition, which should offer a higher reaction specificity. Should be provided with inhibition of nonspecific amplifications, which interfere with quantitative determination, accurate assays over a wide range should be possible. The reagent should allow highly reproducible and reliable real time PCR analyses. 12 The RNase-Free Water should be suitable for use with RNA samples. It should not be prepared using hazardous DEPC (diethylpyrocarbonate), and therefore avoids risk of inhibiting RT-PCR which occur with inhibiting residual DEPC. (10X1mL/Pack) 13 Rectangular Carboy with Stopcock should be of high performance and autoclavable and should be made from Medical Grade PP confirming to Class VI. Autoclavable. (1p/Pack) 14 50X TAE Buffer should be a concentrated stock solution of Tris-Acetate-EDTA buffer, a solution to be used in molecular biology for agarose gel electrophoresis to separate nucleic acids like DNA and RNA. It should contain Tris base, acetic acid, and EDTA. A 50X stock solution should typically contain 2M Tris, 0.05M EDTA, and 1M acetic acid, and should result in a 1X solution of 40mM Tris, 20mM acetic acid, and 1mM EDTA. (200mL) 15 Taq Genotyping Master mix should be at 2X concentration and should be compatible with various equipment for the detection of targets which includes the copy number variants, SNPs, etc. It should be premixed with the ROX and contain the Hot start Taq DNA polymerase with high specificity. The product should be of ultra-pure grade quality which have the capacity of primer probe detection method. (1mL/Pack) 16 Hot Start Taq Master Mix Kit (250 U) Hot Start Taq DNA Polymerase, should contain PCR Buffer that includes the dNTPs. The reagent should be stringent with PCR specificity and reproducibility assay in which low-copy targets are amplified. The Taq DNA polymerase should have the following requirements, Concentration: 5 units/μl; Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescentdNTP/ ddNTP; Extension rate: 2–4 kb/min at 72°C; Half-life: 10 min at 97°C ; 60 min at 94°C; Amplification efficiency: ≥105 fold 5– >3 exonuclease activity: Yes; Extra A addition: Yes 3–>5 exonuclease activity: No; Contaminating nucleases: No; Contaminating RNases: No Contaminating proteases: No; Self-priming activity: No > 17 The miRNA extraction kit should be of high throughput that ensures the isolation of total RNA, including miRNA, from a minimum of 200 μl of sample. The kit should provide a phenol-free extraction protocol. The kit should include UCP columns that go through a rigorous ultraclean production process to ensure highly pure RNA eluates. (50Rxn/Pack)