Name of Work:Outsourcing of consultancy services w.r.t. Bulk RNA sequencing and analysis of mice brain tissue and cells.
Sl. No.
Item Description
1
Appendix A: Description of Services TERMS OF REFERENCE (TOR)Requirement of Services/ Technical specifications
2
Bulk RNA sequencing and analysis of mice brain tissue and cells• RNA isolation and quality check• Library preparationand quality check• Transcriptome (mRNA) sequencing.• Standard analysis transcriptome: perform read QC, alignment/ qualification, then differential expression with DESeq2, edgeR to determine the fold changes needed for downstream pathway analysis. For “pathway changes” in use of DESeq2 + preranked GSEA (Hallmark +KEGG/ Reactome) as the main readout, plus ORA for robustness GAGE/GSVA as a secondary check.
We takes all possible care for accurate & authentic tender information, however Users
are requested to refer Original source of Tender Notice / Tender Document published by
Tender Issuing Agency before taking any call regarding this tender.
