Global Tender Document For Screening/Cultivation Robot And High-Throughput Cultivation System, pune-Maharashtra

Global Tender Document For Screening/Cultivation Robot And High-Throughput Cultivation System; System Capabilities and Technical Specifications Imaging and Detection Capabilities • Must automatically detect colonies of algal, yeast, filamentous fungi, and bacterial organisms. • Must include a minimum of 6 lighting channels, enabling: o Fluorescence imaging across visible and UV spectrum o Detection of fluorophores including GFP, mCherry, and BFP o High-fidelity white light imaging (top-lit and back-lit) • Must include image acquisition with resolution up to 5626 × 4220 pixels. • Full user control of imaging parameters including: o Power, Brightness, Gain, Exposure, Hue, Saturation, and White Balance • Must support manual selection/deselection of colonies after detection. • Must detect colonies ≥ 90 µm in diameter. Pinning (Arraying) Capabilities • Must use a non-metallic disposable picking surface: o Prevents cross-contamination o No requirement for washing or sterilisation procedures • No requirement for organism-specific pin heads: o Must use a standardised plastic picking head • Must support: o Up to 6144 colonies per agar plate o Pinning events under 30 seconds Throughput and Speed • Peak picking/pinning capacity: >800,000 picks/stamping/griding per hour • Must pick and pin colonies: o From agar to agar in ≤ 25 seconds o From agar/liquid to agar/liquid in ≤ 28 seconds • Must carry out 6144 pins in ≤ 25 seconds • Must support replication cycles at ≤ 30 seconds per plate • Must use 3-legged plate grabbing technology for plate positioning with <2 µm position error Precision, Compatibility, and Formats • Must support: o Automatic mating by replica pinning o 96, 384, 1536, and 6144 format array densities • Must support: o SBS-format plates (e.g., Nunc Omnitray, 6–96 well, deep well, PCR plates) Data Management and User Interface • Suitable computer systems to be provided • User interface must be intuitive, with 90% of functions learnable in <10 minutes. • Must support: o Fully customizable plate sectoring templates o Special mode for CSV-based custom protocols (e.g., Excel) o Complete data traceability: CSV exports including colony parameters, coordinates, pre- and post- processing images API and Integration Requirements • Must include a free, supported API (no extra cost) • Must support integration with robotic arms Performance Guarantees • Cross-contamination risk < 0.05% • No ethanol-based cleaning system required • Protective Roller and UV disinfectant light source to sterilize after usage Analysis and Visualisation Tools • Must include: o Advanced visualisation tools (e.g., heatmaps, boxplots, normalisation across plates) o Support for Synthetic Genetic Arrays, Yeast 2-Hybrid, Random Mutagenesis arrays Support and Servicing • Technical support must: o Respond to ≥85% of queries within 24 hours (weekdays) o Respond to ≥43% of queries within 1 hour • Technicians must be trained and certified to support: o Colony picking systems o Colony imaging systems o High-throughput screening robots

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INR 2.25 Crore /-

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