Bids Are Invited For 1. Peptone special Dehydrated Media Bacteriological Grade, Make: BD Difco/ Himedia /Sigma Aldrich 500 Grams 2. Mannitol salt agar Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 500 Grams 3. Hugh Leifson medium Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 100 Grams 4. Blood Agar base, granulated Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 500 Grams 5. Brain Heart Infusion broth Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 500 Grams 6. Brain Heart Infusion Agar Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 500 Grams 7. Baird Parker Agar Base Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 100 Grams 8. Potassium Tellurite 3.5% (1 ml per vial) As supplement for Baird Parker Agar Base Pack of 5 vial of 1ml -Make:BD Difco/ Himedia /Sigma Aldrich 5 vial 9. HiCrome Aureus Agar Base Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 100 Grams 10. Egg Yolk Emulsion As supplement for Baird Parker Agar Base Pack of 50ml x 5vial ;- Make:BD Difco/ Himedia /Sigma Aldrich 5 vial 11. HiCrome MeReSa Agar Base Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 100 Grams 12. MeReSa selective supplement Supplement specifically compatible with HiCrome MeReSa Agar Base Pack of 5 vials - Make:BD Difco/ Himedia /Sigma Aldrich 5 vials 13. Cefoxitin Supplement Supplement specifically compatible with HiCrome MeReSa Agar Base Pack of 5 vials - Make:BD Difco/ Himedia /Sigma Aldrich 5 vials 14. NZCYM Growth Medium Dehydrated Media Bacteriological Grade, Make: BD Difco/ Himedia /Sigma Aldrich 15. NZCYM Growth Agar Dehydrated Media Bacteriological Grade, Make:BD Difco/ Himedia /Sigma Aldrich 500 Grams 16. NZCYM Growth Top Agar Dehydrated Media Bacteriological Grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 500 Grams 17. Gelatin Molecular grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 100 Gram 18. Glucose Analytical grade, 500gm -Make:Sigma/Himedia/ SRL/ Thermo Scientific 500 Gram 19. Tris HCl MB grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 1000 Gram 20. 70% Perchloric acid Analytical grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 1000 ml 21. Anhydrous ferric chloride Analytical grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 100 Gram 22. Magnesium sulphate heptahydrate AR Grade, Make: Sigma/Himedia/ SRL/ Thermo Scientific/ 500 Gram 23. Calcium chloride dihydrate AR Grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 500 Gram 24. Magnesium chloride hexahydrate AR Grade, Make:Sigma/Himedia/ SRL/ Thermo Scientific 500 Gram 25. Potassium permanganate MB /PTC grade, Make: Sigma/Himedia/ SRL/ Thermo Scientific/ 500 Gram 26. Amoxyclav- 10 mcg/disc AR Grade Pack of 5 vials, Make:Sigma/Himedia/ SRL/ Thermo Scientific/ 5 Vials 27. Ampicillin- 25 mcg/disc AR Grade Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 28. Cefotaxime- 10 mcg/disc AR Grade; Pack of 5 vials; - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 29. Cefixime- 10 mcg/disc AR Grade; Pack of 5 vials; - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 30. Ceftriaxone- 10 mcg/disc AR Grade; Pack of 5 vials; - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 31. Ciprofloxacin- 10 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 32. Cloxacillin- 1 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 33. Gentamicin- 50 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 34. Methicillin- 30 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 35. Ofloxacin- 2 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 36. Penicillin- 2 units AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 37. Streptomycin- 25 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 38. Tetracycline- 10 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 39. Cloxacillin- 5 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 40. Enrofloxacin- 5 mcg/disc AR Grade; Pack of 5 vials - Make BD difco/ Himedia/ Sigma Aldrich 5 Vials 41. Wizard DNA Clean-Up System 50 reaction For purification of DNA from bacteriophages (50 reaction)- Make - Promega 1 Pack 42. Freeze Tag, White lable Freeze Tag, White label Size: 25 x 13 mm, 1000 labels per Roll pack form- Make;- Himedia/Genexy/Tarson 2 Rolls 43. Sterile, Disposable L-Spreaders Recommended for spread plate Technique, Individually packed- Make-; Himedia/Genexy/Tarson 250 Piece 44. Cryogenic Vial, 1.8 ml Self-standing Made up of Material- PP/HDPE Sterilized, Graduated 1.8 ml vials - Make; Himedia/Tarson 1000 Vials 45. Parafilm M250 Thermoplastic, colourless & Semi transparent film, used as all Purpose laboratory self sealing film Which is flexible, mouldable and aBarrier to moisture loss, Roll size : 2” x 250’ on 1” dia. Core.- Make; Himedia/Tarson 2 Pack 46. Durapore, Sterile Syringe-driven Filters size 0.22 µm, 33 mm diameter MCE hydrophilic membrane, pore size 0.22 µm, 33 mm diameter, Sterile , Qty. 100 pieces, - Make; Merck Millipore/ Himedia 100 Nos 47. Durapore, Sterile Syringe-driven Filters size 0.45 µm, 33 mm diameter MCE hydrophilic membrane, pore size 0.45 µm, 33 mm diameter, Sterile Qty.100 piece - Make; Merck Millipore/ Himedia 100 Nos 48. Sucrose Analytical Grade 500 gm x 2; Make; Himedia/ SRL 1000 Gram 49. 6 X DNA loading Dye Premix gel loading dye for agarose gene electrophosresis 1 x 5 mL- Make: Thermofisher 5 mL 50. Dneasy Blood and Tissue kit For purification of genomic DNA from blood and tissue samples (250 reaction)- make: Qaigen 250 Sample 51. Gamborg B5 Medium White to off-white, homogenous, free flowing powder Solubility 23.2 gms/litre soluble in distilled water Colour and Clarity Colourless to light yellow, clear solution pH at 25ºC 3.50 - 4.50 -Make; Himedia 50 Liter 52. Murashige and Skoog Modified Medium White to off-white, homogenous, free flowing powder Solubility 34.4 gms/litre soluble in distilled water Colour and Clarity Colourless to light yellow, clear solution pH at 25ºC 3.50 - 4.50 - Make; Himedia 100 Liter 53. Murashige and Skoog Medium White to off-white, homogenous, free flowing powder Solubility 34.4 gms/litre soluble in distilled water Colour and Clarity Colourless to light yellow, clear solution pH at 25ºC 3.50 - 4.50 - Make; Himedia 50 Liter 54. Murashige & Skoog medium including MES buffer. White to off-white, homogenous, free flowing powder, 4.9 gms/litre soluble in distilled water, pH at 25ºC 3.50 - 4.50, Media contain Calcium Chloride,Vitamins, MES and Sucrose.without agar. - Make; Himedia 50 Liter 55. pGEMTEasy Vector system-II 50ng/ul suitable for 20 reactions, to be supplied along with 2X ligation buffer, T4 DNA Ligase, JM109 competent cells and control DNA, - Make Thermoscientific 20 Reactions 56. BpiI (BbsI) (10 U/μL) 200 units, 10 - 12 U/μL where 1unit can digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer. - Make Thermo-scientific 400 Units 57. MspJI Supplied with 10X rCutSmart Buffer, with no nonspecific DNase activity. - Make Thermo-scientific 200 Units 58. RNAse A 2 X 0.7 ml, Ready to use; pH-7.4, solution should be colourless and odourless; DNAse free, proteinase free, specific activity greater than 5000 units per mg - Make Thermo-scientific 1400.0 Microlitre 59. NB media White to off-white, homogeneous, free flowing powder. Solubility: 24.08 gm/litre soluble in distilled water. Colour and Clarity : Colourless to light yellow, clear solution. pH at 25°C : 4.0 ±0.5 of 2.408% w/v dehydrated medium - Make Himedia 60. SgsI (AscI) 300 units, 10 U/μl where 1unit can digest 1 μg of lambda DNA-Cpo fragments in 1 hour at 37 °C in 50 μL of assay buffer - Make Thermo-scientific 300 Units 61. Platinum green PCR Master Mixes Platinum SuperFi II Green PCR Master Mix (2X) contains a ready-to-use mixture of Platinum DNA Polymerase, Platinum Buffer, and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with innovative Buffer, enabling universal primer annealing for the highest success in PCR - Make Thermo-scientific 100 Reaction 62. DreamTaq Green PCR Master Mix 2x green ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. DreamTaq PCR Master Mix (2X) was tested for amplification of 20 kb DNA fragment 200 x50 μL - Make Thermo-scientific 200 Reactions 63. Maxima sybr green The 1 x 5 mL vials contain a 2X mixture of SYBR Green, DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 200 reactions based on a 50 μl reaction volume 1x5 mL.- Make Thermo-scientific 5 mL 64. Methanol Volatile, colourless, flammable liquid with a distinctive alcoholic odour similar to that of ethanol. Use to dissolve various antibiotics and hormones - Make Himedia 2500.0 ml 65. Plant RNA isolation kit Kit contain Mini Spin Columns, Collection Tubes (1.5mL and 2mL), RNase-free Reagents and Buffers. Isolated sample should be Ideal for Northern, Dot and Slot Blotting, End-point RT-PCR, Quantitative, Realtime RT- PCR, Array Analysis. High-quality total RNA in 30 minutes. No phenol/chloroform extraction. No CsCl gradients, no LiCl or ethanol precipitation. Excellent recovery of RNA - Make Thermo-scientific 50 samples 66. X-Gal ≥99.0% Purity, determined by HPLC, dissolved in 20 mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) - Make Thermoscientific 1 gram 67. X-Gluc ≥99.0% Purity, determined by HPLC, dissolved in 0.1 M stock solution in dimethylformamide or dimethylsulfoxide - Make Thermo-scientific 100.0 mg 68. Dimethylsul foxide (DMSO) sequencing-grade dimethylsulfoxide is >99.5% pure DMSO. Density: 1.101 g/mL, Free from particulate matter and Water content: < 0.2% - Make: Himedia 250 mL 69. MOPS Purity > 99%, 100mg powder dissolved in 1lit of solution. pH (10% in water at 25°C) : 3.00 - 5.00 - Make: Himedia 100 Gram 70. D, LPhosphinith ricin White to off-white crystals or powder or solid; Clear solution at 33.3 mg in 1 mL of water; min. 97.00% in HPLC Assay; Melting range- 205 - 217°C; suitable for plant tissue culture - Make: Himedia 500 Milligram 71. MES buffer Purity > 99%, 100mg powder dissolved in 1lit of solution. pH (10% in water at 25°C) : 2.5- 4.00 - Make: Himedia 500 Gram 72. Plant Agar Cream coloured, homogenous, free flowing powder, Hazy solution of 0.8 g in 100ml of water after Boiling Loss on drying : <=10.0% <=6.5% Gelling strength : 800-1100 gm/cm² - Make: Himedia/Duchefa 1 Kg 73. Kinetin >99% purity by HPLC, White to beige powder, Clear solution at 33.3 mg in 1 mL of 1N sodium hydroxide solution - Make: Himedia 5 Gram 74. Syringe Driven Filters Pore size- 0.22 micron; Diameter- 25 mm, Sterile membrane made of Nylon, Not packed individually, without pre-filter - Make: Himedia 300 Pieces 75. RNA extraction kit from plant Coloum based kit for High quality RNA extraction from plant tissues within an hour; kit for 50 samples - Make: Qiagen/Invitrogen/ Thermofisher/ Promega 50 Samples 76. First strand C-DNA synthesis kit c DNA synthesis from mRNA with low quantity for or gene expression analysis, kit for 50 reactions x 2 - Make: Qiagen/Invitrogen/ Thermofisher 100 Reaction 77. Taq DNA Polymerase (2.5 Unit/ μL) Supplied with 10X buffer with and without Mgcl2 (2.5 Unit/ μL), quanitity required 1500 Units - Make:Thermofisher/ Promega/Invitrogen/Takara 1500 Units 78. 10 mM dNTPs Mix Highly stable and greater than 99% purify, dNTPs Mix 10 mM, quanitity required 1 ml Units PCR Grade - Make:Thermofisher/ Promega/ Invitrogen/ Takara 1000 Microlitre 79. 100bp Plus DNA Ladder/ GeneRuler Ready-to-use ladder 100 µg, - Make: Qiagen/Invitrogen/ Thermofisher/ Promega 100 Microgram 80. pGEM-T Easy Vector System I TA Cloning Kit Quick, highly efficient for cloning of PCR products kit for 20 reactions - Make: Promega 20 Reactions 81. NEBridge Golden Gate Enzyme Kit (BsaI-HF v2) MB grade, Enzymes/kit for 20 reactions - Make: New England Biolabs (NEB) 20 Reactions 82. PCR Cloning Kit Molecular Biology grade, kit for 20 reactions - Make: New England Biolabs (NEB) 20 Reactions 83. NEBridge Golden Gate Assembly Kit (BsmBI-v2) Molecular Biology grade, quanitity 20 reactionsMake: New England Biolabs (NEB) 20 Reactions 84. T4 DNA Ligase Molecular Biology grade, quanitity required, 20000 units - Make: New England Biolabs (NEB) 2000 Units 85. Quick Blunting Kit Molecular Biology grade, kit for 20 reactions - Make: New England Biolabs (NEB) 20 Reactions 86. Phusion® High-Fidelity PCR Kit Molecular Biology grade, kit for 50 reactions - Make: New England Biolabs (NEB) 87. Quick CIP Molecular Biology grade, quanitity req.1000 units - Make: New England Biolabs (NEB) 1000 Units 88. BbsI-HF Molecular Biology grade, quanity req. 300 unitsMake: New England Biolabs (NEB) 300 Units 89. BsaI-HFv2 Molecular Biology grade, quanity required 1000 units - Make: New England Biolabs (NEB) 1000 Units 90. T4 Polynucleotide Kinase Molecular Biology grade, quanity required 500 units - Make: New England Biolabs (NEB) 500 Units 91. Antarctic Phosphatase Molecular Biology grade, quantity req. 1000 units - Make: New England Biolabs (NEB) 1000 Units 92. pENTR/D-TOPO Cloning Kit Molecular Biology grade, quanity req. 20 reaction - Make: Thermo fisher/Invitrogen 20 Reactions 93. Gateway LR Clonase II Enzyme mix Molecular Biology grade, quanity req. 20 reaction - Make: Thermo fisher/Invitrogen 20 Reactions 94. Gateway BP Clonase II Enzyme mix Molecular Biology grade, quanity req. 20 reaction - Make: Thermo fisher/Invitrogen 20 Reactions 95. Murashige & Skoog Medium w/ CaCl₂, Vitamins & MES; w/o Sucrose & Agar Plant Tissue culture media, quanity req. 20 Litres - Make: Himedia/SRL 50 Litres 96. L- Proline Additional supplements - Make: Himedia/SRL/ Duchefa 25 Grams 97. Acetosyringone Plant transformation in monocot - Make: Himedia/SRL/ Duchefa 5 Grams 98. Rifampicin (RFP) Antibiotic - Make: Himedia/ Sigma Aldrich/ SRL 5 Milligrams 99. Hygromycin B (HGR) for tissue culture Antibiotic - Make: Himedia/ Sigma Aldrich/ SRL 250 Milligrams 100. Carbenicillin Antibiotic - Make: Himedia/ Sigma Aldrich/ SRL 5 Grams 101. Timentin Antibiotic - Make: Himedia/ Sigma Aldrich/ SRL 1 Grams 102. 3,3-Diaminobenzidine (DAB) pure Staining dye - Make: Himedia/ Sigma Aldrich/ SRL 5 Grams 103. NAA Hormone for plant tissue culture - Make: Himedia/SRL/ Duchefa 100 Grams 104. 2,4 D Hormone for plant tissue culture - Make: Himedia/SRL/ Duchefa 100 Grams 105. 6 BAP Hormone for plant tissue culture - Make: Himedia/SRL/ Duchefa 25 Grams 106. Kinetin Hormone for plant tissue culture - Make: Himedia/SRL/ Duchefa 5 Grams 107. Zeatin Hormone for plant tissue culture - Make: Himedia/SRL/ Duchefa 25 Milligrams 108. Casein hydrosylate Additional supplements for PTC - Make: Himedia/SRL/ Duchefa 500 Gram 109. Spermidine Additional supplements for PTC - Make: Himedia/SRL/ Duchefa 1 Grams 110. MES Buffer Additional supplements for PTC -Make: Himedia/SRL/ Duchefa 100 Grams 111. Kanamycin Antibiotic -Make: Himedia/ Sigma Aldrich/ SRL 25 Gram 112. Sucrose PTC grade pack of 1kg x 5 nos - Make: Himedia/SRL/ Duchefa 5000 Grams 113. 0.22 um syringe filter Consumable items Make: - Merck/ Himedia/ Avanter 100 Pieces 114. 5-Bromo-4-Chloro-3- Indolyl-B-DGlucuronide Cyclohexylammonium Salt (X-Gluc CHX) extrapure, 99% PTC Plant tissue culture/Molecular Biology grade - Make: Himedia/SRL/ Duchefa 100 Milligrams 115. Boric acid Molecular Biology /Plant tissue culture grade - Make: Himedia/SRL/ Duchefa 1000 Grams 116. IPTG Molecular Biology /Plant tissue culture grade - Make: Himedia/SRL/ Duchefa 10 Grams 117. X-Gal Molecular Biology /Plant tissue culture grade - Make: Himedia/SRL/ Duchefa 200 Grams 118. Wizard SV Gel and PCR CleanUp System PCR product purification kit for 50 samples - Make: Promega/Thermo/Qiagen/invitrogen 50 Samples 119. Agar powder, bacteriological grade Agar powder, Bacteriological grade, recommended for routine bacteriological work. Must not form lump in the culture. 500 Grams 120. Starch casein agar media Readymade media for culturing actinomycetes. 500 gram 121. Orthophosphoric acid ≥85% purity 2500.0 ml 122. DreamTaq PCR master Mix 2X concentration, 200X50µL rxns 1000 reactions 123. GeneJet plasmid Miniprep kit 100 prep 100 reactions 124. Daidzein 100 mg; Soluble in DMSO (25 mg/ml), ethanol (2 mg/ml), DMF (10 mg/ml), and 1:10 DMSO: PBS (pH 7.2) (0.15 mg/ml). Insoluble in water; pKa: 7.54 100 mg 125. Genistein 100 mg; Slightly soluble in chlorform:methanol (1:1[v:v]) (10 mg/ml), DMSO (100 mM), hot Water, hot ethanol, hot methanol, soluble in hot 80% ethanol, hot 80% methanol, hot acetone, pyridine, a1:6 solution of DMSO: PBS(pH7.2) (~1 mg/ml), DMF (~30 mg/ml), and 100% ethanol (4 mg/ml). Insoluble in cold water. 100 mg 126. LR Clonase Clonase mix with buffer; separately providing proteinase K solution (2 μg/μL) and a positive control vector. 40 reactions 127. Bradford reagent For rapid, accurate protein estimation, made up of Coomassie Brilliant Blue G-250. Absorbance shift from 465 nm to 595 nm in presence of protein, ready-to-use. standard curve (BSA), 20- 1500 µg/mL range, spectrophotometer/plate reader, faster than Lowry method, free of DNases/RNases, 500 mL 128. Ethanol Analytical reagent ethanol component 99.9% 1000 mL 129. Potato Dextrose Broth, Granulated For cultivation and enumeration of yeasts and moulds. 500 Grams 130. Streptomycin Streptomycin Sulphate, hygroscopic antibiotic, white to light yellow powder, soluble in water (100 mg/mL). Molecular formula: C₂₁H₃₉N₇O₁₂.1.5H₂SO₄, molecular weight: 728.69 g/mol. Potency: 650-850 µg/mg, pH (20% solution, 25°C): 4.50-7.00. Storage: 2- 8°C, shelf life: three years. 10 Grams 131. Standard fluorescein Fluorescein (Practical Grade, Free Acid), Molecular Formula C₂₀H₁₂O₅, Molecular Weight 332.31 g/mol, Appearance Orange to red to maroon powder, Solubility 20 PPM in 0.1N NaOH, clear yellow solution with fluorescence, Absorption Maxima 490 - 499 nm (in 0.1N NaOH), Loss on Drying ≤ 5.00% (105°C, 2 hours), 100 Grams 132. Tris(hydroxymethyl) aminomethane Tris buffer, AR and/or ACS grade, extrapure, for molecular biology, at least 99.9% purity 500 Grams 133. Maleic acid Maleic Acid, AR and/or ACS grade, extrapure, for molecular biology, at least 99.5% purity 100 Grams 134. Citric acid Citric Acid Anhydrous, has a molecular formula of C₆H₈O₇ and a molecular weight of 192.12. AR and/or ACS grade, extrapure, for molecular biology, at least 99.7% purity 500 Grams 135. Boric acid Boric Acid, has a molecular formula of H₃BO₃ and a molecular weight of 61.83. It appears as colorless or white crystals, powder. AR and/or ACS grade, extrapure, for molecular biology, at least 99.5% purity 500 Grams 136. Glacial acetic acid (99%) Acetic acid glacial, is a clear, colorless liquid with a molecular formula C₂H₄O₂ and a molecular weight of 60.05. HPLC grade. Purity is ≥99.90% 137. Triphenyl tetrazolium chloride 2,3,5-Triphenyltetrazolium chloride AR, C₁₉H₁₅ClN, tetrazolium salt, 10 g, solid, white, redox indicator, microbiology, plant physiology, white solid, 334.81 g/mol, biochemical assays, respiration detection, melting point 235°C, plastic Total Quantity : 87192
