Tender For Fplc System Under Buyback Offer, New Delhi-Delhi

Tender For Fplc System Under Buyback Offer Please specify - Make and Model FPLC System: The system should be of state-of-the-art Fast Protein Liquid Chromatography (FPLC) with an ability to support all the following chromatography techniques: Affinity Chromatography, Ion- Exchange chromatography, Size-Exclusion Chromatography & Hydrophobic Interaction Chromatography. Should have provision for attachment of column holders and accessories. Should be upgradable for microscale purification. Compatible with prepacked analytical and preparative columns from GE/Cytiva/BioRad or any other vendor. The system should have an operating flow rate of 0.001 to 15 ml/min or more and should not deviate beyond ±2%. The system should have feature to adjust the flow rate automatically when a run is being performed in a cold room or cold cabinet, when a run is being performed and if there is a back pressure build up. The system should have an automatic pump wash, prime and purge provision. The system should be able to withstand operating pressure of 200 bar (or above). System pump should be high-performance positive displacement binary/reciprocating piston pumps capable of delivering solutions/mixtures with viscosity up to 10.0 or higher cP and have mixer module with magnetic stirrer to generate gradient. The system should have a gradient mixer and be capable of creating a gradient between 0-100%. The system should be supplied with 4 inlet lines or more and allow selection between two A and two B positions. Should have integrated air or separate air sensor(s) in the buffer line(s) to protect the columns. System should allow automated sample loading of large volumes of samples through IMAC, affinity and ion-exchange columns, with pressure and air sensors to protect the column and allow complete sample application without the need to pre-program the sample volume. The system should have a mixer for ensuring mixing of buffers. However, to avoid buffer dilution, the volume of the mixer should not exceed 1.5 mL. The system should have space to keep the buffer containers at a height above the pump, to ensure positive hydrodynamic head. The system should have safety feature to avoid buffer back-mixing during standby state. The system should have provision to pass protein sample into the column directly, bypassing the mixer. System should support more than 3 loops at a time for multiple sample injections and must be capable of automated sample injection with option of loop selection for different sample volumes. The system should be able to read the temperature in the flow path with high accuracy.

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