Tender For Supply,Installation And Commissioning Of Goods; Live-Cell Imaging & Analysis System 1. The Should have automated image acquisition and analysis mode that can operates and captures images from within a standard tissue culture incubator so that precise control of temperature, humidity and other environmental factors such as CO2 and O2 can be maintained. System can provide - Uninterrupted incubation – up to six microplates can be imaged simultaneously without being removed from an incubated environment. Other instruments require cells to be subjected to room temp and environment in order to image multiple vessels in parallel. 2. System optics should be mobile, meaning that the optics move to the areas being imaged. The cell culture vessels remain stationary during this process. Stationary optics and stage driven vessel movement are not acceptable when imaging sensitive or non-adherent cell types. 3. System optics should not need to be adjusted. 4. The System should be capable of imaging in parallel any mixture of 6 assay plates that conform to the ANSI/SLAS standard for assay plates. These include 384-well microplates, 96-well microplates, 48-well plates, 24-well plates, 12-well plates, and 6-well plates. 5. System Cam accommodates the following but is not limited to the following plastic tissue culture vessels: 92.6 cm2 Roboflask, 500 cm2 Tripleflask, 84 cm2 Autoflask, 225 cm2 flasks, 185 cm2 flasks, 182 cm2 flasks, 175 cm2 flasks, 162 cm2 flasks, 150 cm2 flasks, 75 cm2 flasks, 25 cm2 flasks, 35mm dishes, 60 mm dishes, 100mm dishes, 150mm dishes, chambered slides and microslides. 6. The System should provide fully automated, hands-free operation for periods exceeding 25 days and should designed to utilize auto-focus and auto- exposure without intervention during this time period. 7. The software should accommodates the entire user workflow with a single, networked software package that leverages a simple to use, guided user interface. a. The software package should have all the tools necessary to perform automated acquisition and subsequent viewing and analysis for 2D cultures. Purpose-built add-ons should available to easily address specific applications such as cell-by- cell analysis, 3D spheroid cultures, chemotaxis, scratch wound migration, neurite outgrowth, and angiogenesis. b. The software should capable of generating label free, time based, growth curves for cells in 2D and 3D (e.g. spheroid) cultures. c. The software should able to mask, quantify and generate time based curves based on fluorescence metrics from thousands of images using an intuitive interface including but not limited to: Fluorescent Count, Fluorescent Average Area, Fluorescent Total Area, Fluorescent Confluence, Fluorescent Mean Intensity, Fluorescent Average Integrated Intensity, Fluorescent Total Integrated Intensity, and Fluorescent Eccentricity. d. The system should performs whole-well imaging for selected vessels and include software for image navigation and panning. 8. The System should have a fluorescence calibration system that allows for comparison of fluorescencebased metrics derived from images on one instrument to metrics derived from images on another instrument. The calibration system also enables comparison of intensity values for images that are captured with different objectives and at different acquisition times. Last, calibration enables automated correction of camera offset, illumination shape, and background signal from sources such as light leakage and auto-fluorescence of optical elements (e.g., dark-field and flat-field corrections). 9. The System should have an Optical Module which includes high definition phase contrast optics, two fluorescence excitation/emission channels, and the illumination source for these channels. Green/Red (Standard Incucyte® S3; Green: ex441-481nm, em503-544nm; Red: ex567-607nm, em622-704nm). 10. The system should have a light protected imaging environment. That can protects sensitive assays from light and reduces potential experimental artefacts which impact the quality of data. 11. The system should have high definition optics that enable imaging of standard 96- and 384-well tissue culture plates without any sidewall or meniscus effects. 12. The system should have the following objectives on an automated turret: 4x PLAN, 10x PLAN FLUOR, and 20x PLAN FLUOR. 13. The system should have a CMOS detector with low read noise and a linear response to changes in fluorescence. 14. system should have data storage capacity of at least 27 terabytes (TB) in the form of a RAID Array and is expandable to 60 TB with an additional storage module.
