Tender For Purchase Of Consumables For The Project Work Of Dr. Jessy Abraham, Additional Professor Of Biochemistry At Aiims, Raipur Assorted Round Magnetic Stirrer Bar with Pivot Ring, the stir bars should have multifaceted surfaces that add turbulence relative to similar smooth size cylindrical bars. Center molded pivot ring which can minimize the contact area between the bar and the vessel, reduces friction and chattering. PTFE/ALNICO V Magnet. 2 3 Autoclavable Biohazard Bags 8X 12 (100pes) it should be PP Autoclavable. It should possess thick high strength, high temperature resistant, autoclavable bags. They should be clearly labelled with biohazard warnings. Bright red colour for quick identification. 3 1 Magnetic Retriever, Length should be 30cm made up of PTFE/Alnico V Magnet (2pcs) Glass 1000 ml Reagent Bottles Narrow Mouth With Screw Cap, the bottle should be designed with heavier wall thickness, making them mechanically stronger to withstand wet or dry sterilization at high temperatures. 5 Glass 500 ml Reagent Bottles Narrow Mouth With Screw Cap, the bottle should be designed with heavier wall thickness, making them mechanically stronger to withstand wet or dry sterilization at high temperatures, Diethyl Pyrocarbonate (DEPC) for molecular biology, 99%, 100mL which is 6 used as a RNase inhibitor for the molecular biology experiments to avoid the degradation of RNA. 7 Agarose for Nucleic acid electrophoresis (DNase, RNase free) 500g molecular biology grade and which have the capacity of separating the DNA of more than 1000bp Buffer AR, ACS for molecular biology, 99.9% (Tris (Hydroxymethyl) Aminonuthane, Tromethamine) 500g which are free from Dnase. RNases Acetic Acid Cilacial ACS, 99.9%, 2.5L supplied in a brown glass bottle stored in Styrofoam to avoid the direct contact with light EDTA Disodium Salt Dihydrate for molecular hiology, 99.5% 500g, free from DNases, RNases for the purpose of molecular biology experiments Reverse transcriptase Reagent Kit 200Rsm should be designed to perform reverse transcription optimized for real-time RT-PCR. The kit should be fast, efficient cDNA template synthesis for real-time PCR possible. This kits protocol should be simple and suitable for high throughput analysis. The RT Reagent Kit should contain all of the components transcription needed soch for reverse Reverse transcriptase enzyme, RNase inhibitur, random 6-mers, oligo di primer, dNTPs, and reaction buffer. Tase should be robust, versatile, and can be used for reverse transcription of virtually any RNA template, including templates that have a high degree of secondary structure or are GC rich. It should possess the excellent extension capability, CONΝΑ synthesis reactions supposed to be performed at 42°C, minimizing the risk of RNA degradation that oflen occurs at higher temperatures The RNase-Free Water of a aliquoted ImLX10 pack size which is suitable for 2 All India use with RNA samples. It should not be prepared using hazardous DEPC (diethylpyrocarbonate), and there should not be any such contents which have potential to inhibit RT-PCR which occur with inhibiting residual DEPC. 14 Sodium Lauryl Sulphate (SDS, SLS) for molecular biology, 99% (Sodium Dodecyl Sulphate) 500g which are free from RNases and DNases and should be molecular biology grade. 15 16 The total RNA extraction reagent 100mL solution which contains the all the required components at molecular grade and provide the high efficiency RNA with the high purity Chloroform for molecular biology, 99.8% 500ml, capacity free froun DNases, RNases for the purpose of molecular biology experiments 17 Isopropanol (IPA) for molecular biology, 99.8% (Isopropyl alcohol, 2-Propanol) 500ml capacity free from DNases. RNases for the purpose of molecular biology experiments Ethanol at 99.9% absolute 500mL 15 capacity with molecular grade which are suitable for a broad spectrum of classical lab applications, and are frequently used in regulated and highly demanding lab applications. The Ethanol should strictly be compiled with the molecular grade which are completely free from other interfering recipients. 19 The electronic multi-dispensable micro- I pipette which have a volume range of 10- 200 HL which has to be battery powered and rechargeable provided with its respective adaptor for charging and which have the compatibility to use with a universal tip size 2-200 HL, 5-300 HL, *2-20 th & *5-200 prjcE compliant, accuracy± 1.6 HL, graduations 0.2 ulL,, ± 0.20% Coefficient of variation, accuracy± 0. 8 % , s ing le-channel, electronic, ± 0.4 [iL Coefficient of variation. The pipette shaft should be able to unscrewed and should be entirely autoclavable (121 °C). It should be providing the convenient programs for pipetting, reverse pipetting, mixing, GEL-Electrophoresis and dispensing. It should be easily Calibrated for making quick adjustments of the electronic pipette without tools. The electronic micropipette should be low weight with intuitive arrangement of control elements for balanced weight distribution to ensure fatigue-free operation and minimize the risk of RSI (repetitive strain injury). The electronic micropipette should be provided with an adjustable finger rest allows user, whether left- or righthanded, to fine-tune the instrument for an ergonomically natural positioning of the hand,. in relation to the functional elements
