Supply Of Media Components As Per Annexure Ii, :- 1 Mem, Powder, 10 X 1 L Or More 2 Fetal Bovine Serum, Certified, Us Origin, 500Ml Or More 3 Trypsin-Edta ( 0.25% ) , Phenol Red, 500 Ml Or More 4 Antibiotic-Antimycotic ( 100X ) , 100 Ml Or More 5 L-Glutamine ( 200 Mm ) , 100 Ml Or More 6 Dmem, Powder, Low Glucose, Pyruvate, 10 X 1 L Or More 7 Egf Recombinant Human Protein, 100 Μg Or More 8 Glucagon, 1Mg Or More 9 Hydrocortisone, 1G Or More 10 Penicillin-Streptomycin ( 10, 000 U / Ml ) , 100Ml Or More 11 Human Insulin Recombinant Protein, 250Mg Or More 12 Collagenase, Type I From Clostridium Histolyticum, 1G Or More 13 Ebss, No Calcium, No Magnesium, No Phenol Red, 500Ml Or More 14 Lambda Protein Phosphatase, 20Ku Or More 15 2-Propanol, 500Ml Or More 16 Sodium Citrate Tribasic Dihydrate, 1Kg Or More 17 Dab Substrate Powder ( 3, 3-Diaminobenzidine Tetrahydrochloride ) , 10G Or More 18 Agarose, 100G Or More 19 Paraformaldehyde, 1Kg Or More 20 Inorganic Pyrophosphatase From E.Coli, Purity Minimum 90% Or Above & Activity: Minimum 800 Units Per Mg Protein Or Above, 1Mg Or More 21 Fluorescein-Ubiquitin, 500 Μg Or More 22 One-Step Rt-Pcr Kit, 100 Rxns Or More 23 Rapamycin, 5Mg Or More 24 Para-Nitrophenyl Phosphate, 100Ml Or More 25 Trizol Reagent, 200Ml Or More 26 D- ( + ) -Raffinose Pentahydrate, 500G Or More 27 Magnabind Goat Anti-Rabbit Igg, 50Ml Or More 28 Lipofectamine 3000 Transfection Reagent, 0.75Ml Or More 29 Yeast Vacuole Membrane Marker Mdy-64, 1Mg Or More 30 Rpmi 1640 Medium, 1L Or More 31 Trypsin Inhibitor ( Soybean Powder ) , 1G Or More 32 Hbss, No Calcium, No Magnesium, No Phenol Red, 500Ml Or More 33 Bsa Fraction V Igg Free, 100 G Or More 34 Bradford Assay Kit, 450Ml Or More
