Name of Work: Supply and Installation of Inverted Phase Contrast Fluorescence Microscope with Dual Photographic Attachment
Sl. No.
Item Description
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Microscope frame and nosepiece: Two tier frame with high static rigidity, water proof construction to help block penetration of water inside the frame. Frontal Controls for Light path selector 0:100/50:50/100:0, Transmitted light Intensity control and light ON/OFF switch. Binocular observation tube having diopter adjustment function. Provided with paired wide field eyepieces. F.N. 20 or above.Ergonomic design, the microscope should be on-site upgradable to automated live cell imaging, automated DIC & automated multichannel fluorescence imaging. Sextuple (6-position) or better revolving DIC nosepiece.
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Objective and Condenser: Universal Plan Semi Apochromat Phase Objective 4X/0.13, WD 17mmUniversal Plan Semi Apochromat Phase Objective 10X/0.3, WD 10mmLong Working Distance Plan Semi Apochromat Phase Objective 20X,NA 0.45,WD 6.6-7.8Long working distance Plan SemiApochromat Phase Objective 40X, NA 0.6, WD 2.7-4Universal Plan Semi Apochromat Objective 100X/1.3 WD 0.2(spring, oil)Long working distance condenser N.A. 0.55 or higher W.D. 27 mm or higher, with at least 5 positions for BF, PH & DIC. Built in iris. Should be upgradable to motorized universal condenser with NA of 0.55 or higher with motorized polariser IN/OUT facility
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Fluorescence turret & light source and camera: 8 Position fluorescence turret with filter set of DAPI, FITC and TRITC.100watt mercury/ LED based illuminator for fluorescence with 6 megapixel or Higher resolution (Sensor Size: 1/1.8 inch, 7.41 mm × 4.98 mm) 4K CMOS digital Cooled Monocrome camera that allows users to share and collaborate data in fine detail, Focus peacking function,large field of view(FOV) upto 25, with live frame rate of at least 60 fps or above. 0.5X C-mount, USB 3.1 interface.Another Color CMOS Camera with minimum 2160 × 1620 pixels and Sensor size of 1/2.5-inch with dedicated C-Mount should be quoted that can be attached in the second Deck of the microscope and can be operate with the same Analysis S/W.
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Software : Imaging S/W-Multi Chanel Fluorescence, TimeLapse. Movie, counting, interactive measurement, automated wavelength, region and line measurements, Tile and Slice view, encoded device, multiple image overlay. Multi colourimaging, Movie play back
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Computer work station: Branded PC with Intel Core i5, Windows 10/ 11 Professional, RAM Size (GB): 16, 1TB SSD, 22” LED Monitor with UPS, Keyboard & Mouse.
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