Educational and Research Institute Boards / Undertakings / PSU Closing in 0 days TDR #41917263

Tender For Supply Of Laboratory Reagents And Other Consumables For Research Work At Department Of Genetics, Ballygunge Science College Campus Under The University Of Calcutta. , Fugene Hd Transfection Reagent, Make-Promega/ Equivalent, Cell Transfect

Issued by Boards / Undertakings / PSU · Kolkata, West Bengal
Tender Value
Ref. Documents
Estimated cost
Bid Submission
12 Feb 2024
0 days left
EMD
7000
Bank guarantee accepted
Document Fee
Ref. Documents
Non-refundable
Tender Type
Online

Tender Overview

Competition Type
NCB
Bidding Type
Tender
Location / State
Kolkata → West Bengal
EMD Exemption
Not Available
Quantity
Not Available

Project Description

Supply Of Laboratory Reagents And Other Consumables For Research Work At Department Of Genetics, Ballygunge Science College Campus Under The University Of Calcutta. , Fugene Hd Transfection Reagent, Make-Promega/ Equivalent, Cell Transfection Reagent, Can Be Used With Difficult-To-Transfect Cell Lines, Primary Cells, Applicable For Protein And Virus Production. (Specification As Per Nit) , Fetal Bovine Serum, Make-Gibcco/ Equivalent, Brazilian Origin, Sterile-Filtered, Tested For Mammalian Cell Culture.(Specification As Per Nit) , Gotaq Green Master Mix , Make: Promega/ Equivalent, 2X, Green Master Mix For Direct-To-Gel Analysis After Amplification, Should Contain Taq Dna Polymerase, Dntps, Mgcl2 And Reaction Buffer, Available With Nuclease-Free Water.(Specification As Per Nit) , Iscript Cdna Kit, Make- Biorad/ Equivalent, First-Strand Cdna Synthesis Kit, Should Contain 5X Reverse-Transcription Reaction Mix, Reverse Transcriptase, Nuclease-Free Water. (Specification As Per Nit) , Itaq™ Universal Sybr® Green Supermix , Make: Biorad/ Equivalent, Should Contain 2X Qpcr Mix, Containing Dntps, Itaq Dna Polymerase, Mgcl2, Sybr Green I, Enhancers, Stabilizers, And Passive Reference Dyes, Ready-To-Use. (Specification As Per Nit) , Pureyield Plasmid Miniprep Kit, Make: Promega/ Equivalent, Plasmid Miniprep System Fortransfection-Ready Plasmid From Cultures , , Should Contain Cell Lysis Buffer, Neutralization Solution, Endotoxin Removal Wash, Column Wash Solution, Elution Buffer, Columns & Collection Tube. Rapid, 10-Minute Protocol. Can Be Used With Centrifugation Or Vacuum-Based Protocols. (Specification As Per Nit) , Dna Isolation Kit From Sputum, Make: Qiagen/ Equivalent, Dna Isolation Kit From Tissues, Swabs, Csf, Blood, Body Fluids Or Washed Cells From Urine. Spin Column-Based Procedure, Suitable For Genomic Dna, Mitochondrial Dna, Bacterial Dna, Parasite Dna, Viral Dna. (Specification As Per Nit) , Fluorotrans™ Pvdf Transfer Membrane Roll, Make-Pall/Equivalent,Detection System Chemiluminescence,Totalprotein Filter Media Fluorotrans Pvdf Pore Size (Removal Rating) 0.2 ?M Sheet Size Length (Metric Cm) 10 Cm Sheet Size Width (Metric Cm) 15 Cm Typical Thickness (Metric ?M) 127 ?M Pore Size - Removal Rating 0.2 ?M (Specification As Per Nit) , Primers 20-25 Base With Hplc Purification , Make: Idt/ Equivalent, High Performance Liquid Chromatography (Hplc), A Form Of Column Chromatography, Can Be Performed In One Of Two Ways. Reverse-Phase (Rp) Hplc Separates Full-Length Oligo Product From Truncated Products Based On Relative Hydrophobicity. Ion-Exchange (Ie) Hplc Separates Full-Length Oligonucleotides From Truncated Species Based On Relative Charge Difference. Idt Uses Hplc To Purify Unmodified Oligos As Well As Oligos With Complex Modifications Such As Linkers, Spacers, Modified Bases, And Hydrophobic Modifications. The Type Of Hplc Selected Is Dependent On Oligo Sequence Composition And Characteristics. (Specification As Per Nit) , Anti-Beta Actin Antibody, Make: Abcam/ Equivalent, Anti-Beta Actin Antibody, Clone Id: [Mabcam 8226]; Mouse Monoclonal Ab, Suitable For: Icc/If, Ihc-P, Wb, Reacts With: Mouse, Rat, Human; Isotype: Igg1. (Specification As Per Nit) , Pcr Tube 0.2Ml Flat Cap, Make: Biorad/ Equivalent, 0.2 Ml Pcr Tube, Flat Caps, High Profile, Clear, Full-Height Wells; Polypropylene Made With Attached Cap, Free Of Dnase, Rnase, And Human Dna. (Specification As Per Nit)

BOQ

Name of Work: Supply of Laboratory reagents and other consumables for research work at Department of Genetics, Ballygunge Science College Campus under the University of Calcutta.
Sl. No. Item Description
1FuGENE HD Transfection Reagent, Make-Promega/ Equivalent, Cell Transfection Reagent, Can be used with Difficult-to-transfect cell lines, primary cells, Applicable for Protein and virus production. (Specification as per NIT)
2Fetal Bovine Serum, Make-Gibcco/ Equivalent, Brazilian Origin, Sterile-filtered, Tested for Mammalian Cell Culture.(Specification as per NIT)
3GoTaq Green Master Mix , Make: Promega/ Equivalent, 2X, Green master mix for direct-to-gel analysis after amplification, Should contain Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffer, Available with Nuclease-Free Water.(Specification as per NIT)
4ISCRIPT CDNA KIT, Make- Biorad/ Equivalent, First-strand cDNA synthesis kit, Should contain 5x reverse-transcription reaction mix, reverse transcriptase, nuclease-free water. (Specification as per NIT)
5iTaq™ Universal SYBR® Green Supermix , Make: Biorad/ Equivalent, Should contain 2x qPCR mix, containing dNTPs, iTaq DNA Polymerase, MgCl2, SYBR Green I, enhancers, stabilizers, and passive reference dyes, Ready-to-use. (Specification as per NIT)
6PureYield Plasmid Miniprep kit, Make: Promega/ Equivalent, Plasmid Miniprep System forTransfection-Ready Plasmid from Cultures , , Should contain Cell Lysis Buffer, Neutralization Solution, Endotoxin Removal Wash, Column Wash Solution, Elution Buffer, Columns & Collection tube. Rapid, 10-minute protocol. Can be used with centrifugation or vacuum-based protocols. (Specification as per NIT)
7DNA isolation kit from sputum, Make: Qiagen/ Equivalent, DNA isolation kit from tissues, swabs, CSF, blood, body fluids or washed cells from urine. Spin Column-based procedure, Suitable for genomic DNA, mitochondrial DNA, bacterial DNA, parasite DNA, viral DNA. (Specification as per NIT)
8FluoroTrans™ PVDF Transfer Membrane roll, Make-PALL/Equivalent,Detection System Chemiluminescence,TotalProtein Filter Media FluoroTrans PVDF Pore Size (Removal Rating) 0.2 µm Sheet Size Length (Metric cm) 10 cm Sheet Size Width (Metric cm) 15 cm Typical Thickness (Metric µm) 127 µm Pore size - removal rating 0.2 µm (Specification as per NIT)
9Primers 20-25 base with HPLC Purification , Make: IDT/ Equivalent, High performance liquid chromatography (HPLC), a form of column chromatography, can be performed in one of two ways. Reverse-phase (RP) HPLC separates full-length oligo product from truncated products based on relative hydrophobicity. Ion-exchange (IE) HPLC separates full-length oligonucleotides from truncated species based on relative charge difference. IDT uses HPLC to purify unmodified oligos as well as oligos with complex modifications such as linkers, spacers, modified bases, and hydrophobic modifications. The type of HPLC selected is dependent on oligo sequence composition and characteristics. (Specification as per NIT)
10Anti-beta Actin antibody, Make: Abcam/ Equivalent, Anti-beta Actin antibody, Clone Id: [mAbcam 8226]; Mouse monoclonal Ab, Suitable for: ICC/IF, IHC-P, WB, Reacts with: Mouse, Rat, Human; Isotype: IgG1. (Specification as per NIT)
11PCR Tube 0.2ml flat cap, Make: Biorad/ Equivalent, 0.2 ml PCR Tube, Flat Caps, high profile, clear, full-height wells; polypropylene made with attached cap, free of DNase, RNase, and human DNA. (Specification as per NIT)

AI Tender Summary

OUR REF NO 41917263
AUTHORITY Boards / Undertakings / PSU
TENDER VALUE Ref. Documents
LAST DATE 12-02-2024
Authority
Authority Name University Of Calcutta
Work Description Supply Of Laboratory Reagents And Other Consumables For Research Work At Department Of Genetics, Ballygunge Science College Campus Under The University Of Calcutta. , Fugene Hd Transfection Reagent, Make-Promega/ Equivalent, Cell Transfection Reagent, Can Be Used With Difficult-To-Transfect Cell Lines, Primary Cells, Applicable For Protein And Virus Production. (Specification As Per Nit) , Fetal Bovine Serum, Make-Gibcco/ Equivalent, Brazilian Origin, Sterile-Filtered, Tested For Mammalian Cell Culture.(Specification As Per Nit) , Gotaq Green Master Mix , Make: Promega/ Equivalent, 2X, Green Master Mix For Direct-To-Gel Analysis After Amplification, Should Contain Taq Dna Polymerase, Dntps, Mgcl2 And Reaction Buffer, Available With Nuclease-Free Water.(Specification As Per Nit) , Iscript Cdna Kit, Make- Biorad/ Equivalent, First-Strand Cdna Synthesis Kit, Should Contain 5X Reverse-Transcription Reaction Mix, Reverse Transcriptase, Nuclease-Free Water. (Specification As Per Nit) , Itaq™ Universal Sybr® Green Supermix , Make: Biorad/ Equivalent, Should Contain 2X Qpcr Mix, Containing Dntps, Itaq Dna Polymerase, Mgcl2, Sybr Green I, Enhancers, Stabilizers, And Passive Reference Dyes, Ready-To-Use. (Specification As Per Nit) , Pureyield Plasmid Miniprep Kit, Make: Promega/ Equivalent, Plasmid Miniprep System Fortransfection-Ready Plasmid From Cultures , , Should Contain Cell Lysis Buffer, Neutralization Solution, Endotoxin Removal Wash, Column Wash Solution, Elution Buffer, Columns & Collection Tube. Rapid, 10-Minute Protocol. Can Be Used With Centrifugation Or Vacuum-Based Protocols. (Specification As Per Nit) , Dna Isolation Kit From Sputum, Make: Qiagen/ Equivalent, Dna Isolation Kit From Tissues, Swabs, Csf, Blood, Body Fluids Or Washed Cells From Urine. Spin Column-Based Procedure, Suitable For Genomic Dna, Mitochondrial Dna, Bacterial Dna, Parasite Dna, Viral Dna. (Specification As Per Nit) , Fluorotrans™ Pvdf Transfer Membrane Roll, Make-Pall/Equivalent,Detection System Chemiluminescence,Totalprotein Filter Media Fluorotrans Pvdf Pore Size (Removal Rating) 0.2 ?M Sheet Size Length (Metric Cm) 10 Cm Sheet Size Width (Metric Cm) 15 Cm Typical Thickness (Metric ?M) 127 ?M Pore Size - Removal Rating 0.2 ?M (Specification As Per Nit) , Primers 20-25 Base With Hplc Purification , Make: Idt/ Equivalent, High Performance Liquid Chromatography (Hplc), A Form Of Column Chromatography, Can Be Performed In One Of Two Ways. Reverse-Phase (Rp) Hplc Separates Full-Length Oligo Product From Truncated Products Based On Relative Hydrophobicity. Ion-Exchange (Ie) Hplc Separates Full-Length Oligonucleotides From Truncated Species Based On Relative Charge Difference. Idt Uses Hplc To Purify Unmodified Oligos As Well As Oligos With Complex Modifications Such As Linkers, Spacers, Modified Bases, And Hydrophobic Modifications. The Type Of Hplc Selected Is Dependent On Oligo Sequence Composition And Characteristics. (Specification As Per Nit) , Anti-Beta Actin Antibody, Make: Abcam/ Equivalent, Anti-Beta Actin Antibody, Clone Id: [Mabcam 8226]; Mouse Monoclonal Ab, Suitable For: Icc/If, Ihc-P, Wb, Reacts With: Mouse, Rat, Human; Isotype: Igg1. (Specification As Per Nit) , Pcr Tube 0.2Ml Flat Cap, Make: Biorad/ Equivalent, 0.2 Ml Pcr Tube, Flat Caps, High Profile, Clear, Full-Height Wells; Polypropylene Made With Attached Cap, Free Of Dnase, Rnase, And Human Dna. (Specification As Per Nit)
Basic Detail
Tender No MSG/WBDSTB/ET-1/23-24
Bidding Type Tender
Location
City Kolkata
State West Bengal
Key Dates
Publish Date 30 Jan 2024
Submission Date 12 Feb 2024
Open Date 15 Feb 2024
Finance
Tender Value Ref. Documents
Tender Fee Ref. Documents
EMD 7000
Exemption Not Available
Document List
9d525eb4-6d67-405e-98c5-c1b2268610c6.html
BOQ_1421420.xls
Tendernotice_1.pdf

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Tender Timeline

Jan 30, 2024
11:30 IST

Tender Published

Tender notice published.

Completed
Feb 12, 2024
17:00 IST

Bid Submission Deadline

Online submission via eProcurement portal.

Completed
Feb 15, 2024

Bid Opening Date

Technical bids will be opened and evaluated.

Completed

Tender Documents

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html

9d525eb4-6d67-405e-98c5-c1b2268610c6.html

xls

BOQ_1421420.xls

pdf

Tendernotice_1.pdf

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